È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Introduction
- 01:00Preparation of Buffers and Collection Plates
- 01:36Preparation of Primary Preadipocyte Samples
- 02:15Preparation of Ex Vivo Culture Samples
- 03:44Lipolysis Assay
- 04:33Free Fatty Acid (FFA) Colorimetric Assay
- 05:46Glycerol Colorimetric Assay
- 06:34Calculation of Lipolytic Rate
- 07:18Results: Basal and Stimulated Lipolytic Rates of In Vitro Differentiated Preadipocytes and Ex Vivo Adipose Tissue
- 09:09Conclusion
