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分离、表征和总 DNA 提取以鉴定真菌异养植物中的内生真菌
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Biologia
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JoVE Journal Biologia
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
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分离、表征和总 DNA 提取以鉴定真菌异养植物中的内生真菌

DOI:
10.3791/65135-v

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06:53 min

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May 05, 2023

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, ,
1LabPlaM – Mycoheterotrophic Plants Research Lab, Department of Plant Biology,State University of Campinas (UNICAMP)

Capitoli

  • 00:00Introduction
  • 00:21Isolation of Endophytic Fungi Associated with Plant Organs
  • 03:12Preservation of Purified Fungal Isolates
  • 04:01Micromorphological Characterization (Hyphal Morphology) of Filamentous Fungi
  • 05:10Results: Isolation and Macromorphology Evaluation of Endophytic Fungi Associated with Mycoheterotrophic Plants
  • 06:33Conclusion

Summary

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本文旨在为植物相关内生真菌的分离、分离株的长期保存、形态学表征和总 DNA 提取提供详细和充分的方案，以便随后进行分子鉴定和宏基因组分析。

Tags

Endophytic FungiMycoheterotrophic PlantsFungal IsolationFungal CharacterizationTotal DNA ExtractionPDA Culture MediumAntibiotic SupplementationSuperficial DisinfectionColony PurificationAA MediumFungal IdentificationFungal Subculturing

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