Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

Published: January 05, 2024

doi:

10.3791/201505

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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Keywords: Epitope Tag Protein Precipitation Immunoblotting Protein-protein Interaction Centrifugation Protein Lysis Buffer Protease Inhibitor Sample Buffer SDS-PAGE PVDF Membrane Primary Antibody DYKDDDDK-PRDM16 HA-EHMT1 HEK 293 Cells

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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies. J. Vis. Exp. (Pending Publication), e201505, doi:10.3791/201505 (2024).

Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

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