Dario R. Alessi MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences University of Dundee Biography Publications Institution JoVE Articles Dario R. Alessi has not added a biography. If you are Dario R. Alessi and would like to personalize this page please email our Author Liaison for assistance. Publications Advances in Elucidating the Function of Leucine-rich Repeat Protein Kinase-2 in Normal Cells and Parkinson's disease Current Opinion in Cell Biology. Feb, 2020 | Pubmed ID: 32036294 Structural Basis for Rab8a Recruitment of RILPL2 Via LRRK2 Phosphorylation of Switch 2 Structure (London, England : 1993). Feb, 2020 | Pubmed ID: 32017888 Phosphoproteomics Reveals That the HVPS34 Regulated SGK3 Kinase Specifically Phosphorylates Endosomal Proteins Including Syntaxin-7, Syntaxin-12, RFIP4 and WDR44 The Biochemical Journal. Oct, 2019 | Pubmed ID: 31665227 PPM1H Phosphatase Counteracts LRRK2 Signaling by Selectively Dephosphorylating Rab Proteins ELife. 10, 2019 | Pubmed ID: 31663853 Membrane Association but Not Identity is Required for LRRK2 Activation and Phosphorylation of Rab GTPases The Journal of Cell Biology. Dec, 2019 | Pubmed ID: 31624137 Design and Characterization of SGK3-PROTAC1, an Isoform Specific SGK3 Kinase PROTAC Degrader ACS Chemical Biology. 09, 2019 | Pubmed ID: 31461270 Rapid and Reversible Knockdown of Endogenously Tagged Endosomal Proteins Via an Optimized HaloPROTAC Degrader ACS Chemical Biology. 05, 2019 | Pubmed ID: 30978004 Crystal Structure of the WD40 Domain Dimer of LRRK2 Proceedings of the National Academy of Sciences of the United States of America. 01, 2019 | Pubmed ID: 30635421 Discovery of Potent and Selective 5-azaindazole Inhibitors of Leucine-rich Repeat Kinase 2 (LRRK2) - Part 1 Bioorganic & Medicinal Chemistry Letters. 02, 2019 | Pubmed ID: 30554956 A Pathway for Parkinson's Disease LRRK2 Kinase to Block Primary Cilia and Sonic Hedgehog Signaling in the Brain ELife. 11, 2018 | Pubmed ID: 30398148 Nigrostriatal Pathology with Reduced Astrocytes in LRRK2 S910/S935 Phosphorylation Deficient Knockin Mice Neurobiology of Disease. 12, 2018 | Pubmed ID: 30194047 PP1 Phosphatase Complexes: Undruggable No Longer Cell. 08, 2018 | Pubmed ID: 30142342 Structural and Atropisomeric Factors Governing the Selectivity of Pyrimido-benzodiazipinones As Inhibitors of Kinases and Bromodomains ACS Chemical Biology. 09, 2018 | Pubmed ID: 30102854 LRRK2 Activation in Idiopathic Parkinson's Disease Science Translational Medicine. 07, 2018 | Pubmed ID: 30045977 LRRK2 is a Negative Regulator of Phagosome Maturation in Macrophages The EMBO Journal. Jun, 2018 | Pubmed ID: 29789389 The Parkinson's Disease VPS35[D620N] Mutation Enhances LRRK2-mediated Rab Protein Phosphorylation in Mouse and Human The Biochemical Journal. 06, 2018 | Pubmed ID: 29743203 LRRK2 Kinase in Parkinson's Disease Science (New York, N.Y.). Apr, 2018 | Pubmed ID: 29622645 Rab29 Activation of the Parkinson's Disease-associated LRRK2 Kinase The EMBO Journal. 01, 2018 | Pubmed ID: 29212815 Mechanism of Activation of SGK3 by Growth Factors Via the Class 1 and Class 3 PI3Ks The Biochemical Journal. 01, 2018 | Pubmed ID: 29150437 Development of Phospho-specific Rab Protein Antibodies to Monitor Activity of the LRRK2 Parkinson's Disease Kinase The Biochemical Journal. 01, 2018 | Pubmed ID: 29127256 Interrogating Parkinson's Disease LRRK2 Kinase Pathway Activity by Assessing Rab10 Phosphorylation in Human Neutrophils The Biochemical Journal. 01, 2018 | Pubmed ID: 29127255 Systematic Proteomic Analysis of LRRK2-mediated Rab GTPase Phosphorylation Establishes a Connection to Ciliogenesis ELife. 11, 2017 | Pubmed ID: 29125462 USP7 Small-molecule Inhibitors Interfere with Ubiquitin Binding Nature. 10, 2017 | Pubmed ID: 29045385 Homo-PROTACs: Bivalent Small-molecule Dimerizers of the VHL E3 Ubiquitin Ligase to Induce Self-degradation Nature Communications. 10, 2017 | Pubmed ID: 29018234 Vomocytosis of Live Pathogens from Macrophages is Regulated by the Atypical MAP Kinase ERK5 Science Advances. 08, 2017 | Pubmed ID: 28835924 Integrated Single-cell Genetic and Transcriptional Analysis Suggests Novel Drivers of Chronic Lymphocytic Leukemia Genome Research. 08, 2017 | Pubmed ID: 28679620 Small-Molecule Inhibitors of LRRK2 Advances in Neurobiology. 2017 | Pubmed ID: 28353288 Regulation of Membrane Ruffling by Polarized STIM1 and ORAI1 in Cortactin-rich Domains Scientific Reports. 03, 2017 | Pubmed ID: 28341841 B-cell-intrinsic Function of TAPP Adaptors in Controlling Germinal Center Responses and Autoantibody Production in Mice European Journal of Immunology. 02, 2017 | Pubmed ID: 27859053 Functional Kinomics Establishes a Critical Node of Volume-sensitive Cation-Cl Cotransporter Regulation in the Mammalian Brain Scientific Reports. 10, 2016 | Pubmed ID: 27782176 The HVps34-SGK3 Pathway Alleviates Sustained PI3K/Akt Inhibition by Stimulating MTORC1 and Tumour growth The EMBO Journal. 09, 2016 | Pubmed ID: 27481935 Phos-tag Analysis of Rab10 Phosphorylation by LRRK2: a Powerful Assay for Assessing Kinase Function and Inhibitors The Biochemical Journal. Sep, 2016 | Pubmed ID: 27474410 PDK1-SGK1 Signaling Sustains AKT-Independent MTORC1 Activation and Confers Resistance to PI3Kα Inhibition Cancer Cell. 08, 2016 | Pubmed ID: 27451907 Phosphoproteomics Reveals That Parkinson's Disease Kinase LRRK2 Regulates a Subset of Rab GTPases ELife. Jan, 2016 | Pubmed ID: 26824392 Discovery of a Pyrrolopyrimidine (JH-II-127), a Highly Potent, Selective, and Brain Penetrant LRRK2 Inhibitor ACS Medicinal Chemistry Letters. May, 2015 | Pubmed ID: 26005538 Critical Role of the SPAK Protein Kinase CCT Domain in Controlling Blood Pressure Human Molecular Genetics. Aug, 2015 | Pubmed ID: 25994507 Phosphorylation of Synaptic Vesicle Protein 2A at Thr84 by Casein Kinase 1 Family Kinases Controls the Specific Retrieval of Synaptotagmin-1 The Journal of Neuroscience : the Official Journal of the Society for Neuroscience. Feb, 2015 | Pubmed ID: 25673844 USP45 Deubiquitylase Controls ERCC1-XPF Endonuclease-mediated DNA Damage Responses The EMBO Journal. Feb, 2015 | Pubmed ID: 25538220 A Next-generation Dual-recombinase System for Time- and Host-specific Targeting of Pancreatic Cancer Nature Medicine. Nov, 2014 | Pubmed ID: 25326799 Characterization of VPS34-IN1, a Selective Inhibitor of Vps34, Reveals That the Phosphatidylinositol 3-phosphate-binding SGK3 Protein Kinase is a Downstream Target of Class III Phosphoinositide 3-kinase The Biochemical Journal. Nov, 2014 | Pubmed ID: 25177796 Screening of DUB Activity and Specificity by MALDI-TOF Mass Spectrometry Nature Communications. Aug, 2014 | Pubmed ID: 25159004 The WNK-SPAK/OSR1 Pathway: Master Regulator of Cation-chloride Cotransporters Science Signaling. Jul, 2014 | Pubmed ID: 25028718 Interplay Between Polo Kinase, LKB1-activated NUAK1 Kinase, PP1βMYPT1 Phosphatase Complex and the SCFβTrCP E3 Ubiquitin Ligase The Biochemical Journal. Jul, 2014 | Pubmed ID: 24785407 Parkin is Activated by PINK1-dependent Phosphorylation of Ubiquitin at Ser65 The Biochemical Journal. May, 2014 | Pubmed ID: 24660806 Kinase and Channel Activity of TRPM6 Are Co-ordinated by a Dimerization Motif and Pocket Interaction The Biochemical Journal. Jun, 2014 | Pubmed ID: 24650431 Phosphorylation of Parkin at Serine65 is Essential for Activation: Elaboration of a Miro1 Substrate-based Assay of Parkin E3 Ligase Activity Open Biology. Mar, 2014 | Pubmed ID: 24647965 Structural and Biochemical Characterization of the KLHL3-WNK Kinase Interaction Important in Blood Pressure Regulation The Biochemical Journal. Jun, 2014 | Pubmed ID: 24641320 The WNK-regulated SPAK/OSR1 Kinases Directly Phosphorylate and Inhibit the K+-Cl- Co-transporters The Biochemical Journal. Mar, 2014 | Pubmed ID: 24393035 Investigation of LKB1 Ser431 Phosphorylation and Cys433 Farnesylation Using Mouse Knockin Analysis Reveals an Unexpected Role of Prenylation in Regulating AMPK Activity The Biochemical Journal. Feb, 2014 | Pubmed ID: 24295069 Characterization of WZ4003 and HTH-01-015 As Selective Inhibitors of the LKB1-tumour-suppressor-activated NUAK Kinases The Biochemical Journal. Jan, 2014 | Pubmed ID: 24171924 Structural Determinants for ERK5 (MAPK7) and Leucine Rich Repeat Kinase 2 Activities of Benzo[e]pyrimido-[5,4-b]diazepine-6(11H)-ones European Journal of Medicinal Chemistry. 2013 | Pubmed ID: 24239623 N-terminal Serine Dephosphorylation is Required for KCC3 Cotransporter Full Activation by Cell Swelling The Journal of Biological Chemistry. Nov, 2013 | Pubmed ID: 24043619 LKB1 and AMPK and the Cancer-metabolism Link - Ten Years After BMC Biology. Apr, 2013 | Pubmed ID: 23587167 Elevated SGK1 Predicts Resistance of Breast Cancer Cells to Akt Inhibitors The Biochemical Journal. Jun, 2013 | Pubmed ID: 23581296 Comprehensive Characterization and Optimization of Anti-LRRK2 (leucine-rich Repeat Kinase 2) Monoclonal Antibodies The Biochemical Journal. Jul, 2013 | Pubmed ID: 23560750 The CUL3-KLHL3 E3 Ligase Complex Mutated in Gordon's Hypertension Syndrome Interacts with and Ubiquitylates WNK Isoforms: Disease-causing Mutations in KLHL3 and WNK4 Disrupt Interaction The Biochemical Journal. Apr, 2013 | Pubmed ID: 23387299 Development of an Enzyme-linked Immunosorbent Assay for Detection of Cellular and in Vivo LRRK2 S935 Phosphorylation Journal of Pharmaceutical and Biomedical Analysis. Mar, 2013 | Pubmed ID: 23313773 Structural Insights into the Activation of MST3 by MO25 Biochemical and Biophysical Research Communications. Feb, 2013 | Pubmed ID: 23296203 Kinase Drug Discovery--what's Next in the Field? ACS Chemical Biology. Jan, 2013 | Pubmed ID: 23276252 Kinase Inhibitors Arrest Neurodegeneration in Cell and C. Elegans Models of LRRK2 Toxicity Human Molecular Genetics. Jan, 2013 | Pubmed ID: 23065705 A Minor Role of WNK3 in Regulating Phosphorylation of Renal NKCC2 and NCC Co-transporters in Vivo Biology Open. Feb, 2012 | Pubmed ID: 23213404 Brain Penetrant LRRK2 Inhibitor ACS Medicinal Chemistry Letters. Aug, 2012 | Pubmed ID: 23066449 Akt is Efficiently Activated by PIF-pocket- and PtdIns(3,4,5)P3-dependent Mechanisms Leading to Resistance to PDK1 Inhibitors The Biochemical Journal. Dec, 2012 | Pubmed ID: 23030823 Phosphatidylinositol 3-kinase/Akt Signaling Pathway Activates the WNK-OSR1/SPAK-NCC Phosphorylation Cascade in Hyperinsulinemic Db/db Mice Hypertension (Dallas, Tex. : 1979). Oct, 2012 | Pubmed ID: 22949526 GSK2578215A; a Potent and Highly Selective 2-arylmethyloxy-5-substitutent-N-arylbenzamide LRRK2 Kinase Inhibitor Bioorganic & Medicinal Chemistry Letters. Sep, 2012 | Pubmed ID: 22863203 PINK1 is Activated by Mitochondrial Membrane Potential Depolarization and Stimulates Parkin E3 Ligase Activity by Phosphorylating Serine 65 Open Biology. May, 2012 | Pubmed ID: 22724072 The IkappaB Kinase Family Phosphorylates the Parkinson's Disease Kinase LRRK2 at Ser935 and Ser910 During Toll-like Receptor Signaling PloS One. 2012 | Pubmed ID: 22723946 Activation of the Renal Na+:Cl- Cotransporter by Angiotensin II is a WNK4-dependent Process Proceedings of the National Academy of Sciences of the United States of America. May, 2012 | Pubmed ID: 22550170 Aspirin Inhibits MTOR Signaling, Activates AMP-activated Protein Kinase, and Induces Autophagy in Colorectal Cancer Cells Gastroenterology. Jun, 2012 | Pubmed ID: 22406476 Characterization of TAE684 As a Potent LRRK2 Kinase Inhibitor Bioorganic & Medicinal Chemistry Letters. Mar, 2012 | Pubmed ID: 22335897 Discovery of Potent and Selective Covalent Inhibitors of JNK Chemistry & Biology. Jan, 2012 | Pubmed ID: 22284361 SPAK/OSR1 Regulate NKCC1 and WNK Activity: Analysis of WNK Isoform Interactions and Activation by T-loop Trans-autophosphorylation The Biochemical Journal. Jan, 2012 | Pubmed ID: 22032326 Discovery of Catalytically Active Orthologues of the Parkinson's Disease Kinase PINK1: Analysis of Substrate Specificity and Impact of Mutations Open Biology. Nov, 2011 | Pubmed ID: 22645651 TTBK2 Kinase Substrate Specificity and the Impact of Spinocerebellar-ataxia-causing Mutations on Expression, Activity, Localization and Development The Biochemical Journal. Jul, 2011 | Pubmed ID: 21548880 MO25 is a Master Regulator of SPAK/OSR1 and MST3/MST4/YSK1 Protein Kinases The EMBO Journal. May, 2011 | Pubmed ID: 21423148 Protor-1 is Required for Efficient MTORC2-mediated Activation of SGK1 in the Kidney The Biochemical Journal. May, 2011 | Pubmed ID: 21413931 Characterization of a Selective Inhibitor of the Parkinson's Disease Kinase LRRK2 Nature Chemical Biology. Apr, 2011 | Pubmed ID: 21378983 Regulation of the NKCC2 Ion Cotransporter by SPAK-OSR1-dependent and -independent Pathways Journal of Cell Science. Mar, 2011 | Pubmed ID: 21321328 Role of TAPP1 and TAPP2 Adaptor Binding to PtdIns(3,4)P2 in Regulating Insulin Sensitivity Defined by Knock-in Analysis The Biochemical Journal. Mar, 2011 | Pubmed ID: 21204784 Characterization of GSK2334470, a Novel and Highly Specific Inhibitor of PDK1 The Biochemical Journal. Jan, 2011 | Pubmed ID: 21087210 How Moderate Changes in Akt T-loop Phosphorylation Impact on Tumorigenesis and Insulin Resistance Disease Models & Mechanisms. Jan, 2011 | Pubmed ID: 20959631 Uncoupling the Warburg Effect from Cancer Proceedings of the National Academy of Sciences of the United States of America. Nov, 2010 | Pubmed ID: 21041650 Temporal Differences in the Dependency on Phosphoinositide-dependent Kinase 1 Distinguish the Development of Invariant Valpha14 NKT Cells and Conventional T Cells Journal of Immunology (Baltimore, Md. : 1950). Nov, 2010 | Pubmed ID: 20944007 Characterization of PF-4708671, a Novel and Highly Specific Inhibitor of P70 Ribosomal S6 Kinase (S6K1) The Biochemical Journal. Oct, 2010 | Pubmed ID: 20704563 Inhibition of LRRK2 Kinase Activity Leads to Dephosphorylation of Ser(910)/Ser(935), Disruption of 14-3-3 Binding and Altered Cytoplasmic Localization The Biochemical Journal. Sep, 2010 | Pubmed ID: 20659021 14-3-3 Binding to LRRK2 is Disrupted by Multiple Parkinson's Disease-associated Mutations and Regulates Cytoplasmic Localization The Biochemical Journal. Sep, 2010 | Pubmed ID: 20642453 New Roles for the LKB1-NUAK Pathway in Controlling Myosin Phosphatase Complexes and Cell Adhesion Science Signaling. Mar, 2010 | Pubmed ID: 20354225 Role of the WNK-activated SPAK Kinase in Regulating Blood Pressure EMBO Molecular Medicine. Feb, 2010 | Pubmed ID: 20091762 The Nuts and Bolts of AGC Protein Kinases Nature Reviews. Molecular Cell Biology. Jan, 2010 | Pubmed ID: 20027184 Structure of the LKB1-STRAD-MO25 Complex Reveals an Allosteric Mechanism of Kinase Activation Science (New York, N.Y.). Dec, 2009 | Pubmed ID: 19892943 Substrate Specificity and Inhibitors of LRRK2, a Protein Kinase Mutated in Parkinson's Disease The Biochemical Journal. Oct, 2009 | Pubmed ID: 19740074 New Users of Metformin Are at Low Risk of Incident Cancer: a Cohort Study Among People with Type 2 Diabetes Diabetes Care. Sep, 2009 | Pubmed ID: 19564453 ATP and MO25alpha Regulate the Conformational State of the STRADalpha Pseudokinase and Activation of the LKB1 Tumour Suppressor PLoS Biology. Jun, 2009 | Pubmed ID: 19513107 Ku-0063794 is a Specific Inhibitor of the Mammalian Target of Rapamycin (mTOR) The Biochemical Journal. Jun, 2009 | Pubmed ID: 19402821 New Insights into MTOR Signaling: MTORC2 and Beyond Science Signaling. Apr, 2009 | Pubmed ID: 19383978 Angiotensin II Signaling Increases Activity of the Renal Na-Cl Cotransporter Through a WNK4-SPAK-dependent Pathway Proceedings of the National Academy of Sciences of the United States of America. Mar, 2009 | Pubmed ID: 19240212 New Anti-cancer Role for PDK1 Inhibitors: Preventing Resistance to Tamoxifen The Biochemical Journal. Jan, 2009 | Pubmed ID: 19061482 Small-molecule Inhibitors of PDK1 ChemMedChem. Dec, 2008 | Pubmed ID: 18972468 MTOR Complex 2 (mTORC2) Controls Hydrophobic Motif Phosphorylation and Activation of Serum- and Glucocorticoid-induced Protein Kinase 1 (SGK1) The Biochemical Journal. Dec, 2008 | Pubmed ID: 18925875 The Regulation of Salt Transport and Blood Pressure by the WNK-SPAK/OSR1 Signalling Pathway Journal of Cell Science. Oct, 2008 | Pubmed ID: 18843116 Novel Role for the LKB1 Pathway in Controlling Monocarboxylate Fuel Transporters The Journal of Cell Biology. Oct, 2008 | Pubmed ID: 18838550 Structure of the OSR1 Kinase, a Hypertension Drug Target Proteins. Dec, 2008 | Pubmed ID: 18831043 Use of Akt Inhibitor and a Drug-resistant Mutant Validates a Critical Role for Protein Kinase B/Akt in the Insulin-dependent Regulation of Glucose and System A Amino Acid Uptake The Journal of Biological Chemistry. Oct, 2008 | Pubmed ID: 18669636 Lithium Prevents and Ameliorates Experimental Autoimmune Encephalomyelitis Journal of Immunology (Baltimore, Md. : 1950). Jul, 2008 | Pubmed ID: 18566399 Important Role of the LKB1-AMPK Pathway in Suppressing Tumorigenesis in PTEN-deficient Mice The Biochemical Journal. Jun, 2008 | Pubmed ID: 18387000 Mutation of the PDK1 PH Domain Inhibits Protein Kinase B/Akt, Leading to Small Size and Insulin Resistance Molecular and Cellular Biology. May, 2008 | Pubmed ID: 18347057 Activation of the Thiazide-sensitive Na+-Cl- Cotransporter by the WNK-regulated Kinases SPAK and OSR1 Journal of Cell Science. Mar, 2008 | Pubmed ID: 18270262 Control of AMPK-related Kinases by USP9X and Atypical Lys(29)/Lys(33)-linked Polyubiquitin Chains The Biochemical Journal. Apr, 2008 | Pubmed ID: 18254724 The Selectivity of Protein Kinase Inhibitors: a Further Update The Biochemical Journal. Dec, 2007 | Pubmed ID: 17850214 Structural Insights into the Recognition of Substrates and Activators by the OSR1 Kinase EMBO Reports. Sep, 2007 | Pubmed ID: 17721439 Characterization of a Protein Kinase B Inhibitor in Vitro and in Insulin-treated Liver Cells Diabetes. Sep, 2007 | Pubmed ID: 17563061 Identification of Protor As a Novel Rictor-binding Component of MTOR Complex-2 The Biochemical Journal. Aug, 2007 | Pubmed ID: 17461779 LRRK2 Phosphorylates Moesin at Threonine-558: Characterization of How Parkinson's Disease Mutants Affect Kinase Activity The Biochemical Journal. Jul, 2007 | Pubmed ID: 17447891 Regulation of Activity and Localization of the WNK1 Protein Kinase by Hyperosmotic Stress The Journal of Cell Biology. Jan, 2007 | Pubmed ID: 17190791 BI-D1870 is a Specific Inhibitor of the P90 RSK (ribosomal S6 Kinase) Isoforms in Vitro and in Vivo The Biochemical Journal. Jan, 2007 | Pubmed ID: 17040210 3'Phosphoinositide-dependent Kinase-1 is Essential for Ischemic Preconditioning of the Myocardium FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Dec, 2006 | Pubmed ID: 17077284 Regulation of the Polarity Kinases PAR-1/MARK by 14-3-3 Interaction and Phosphorylation Journal of Cell Science. Oct, 2006 | Pubmed ID: 16968750 Evaluation of Approaches to Generation of Tissue-specific Knock-in Mice The Journal of Biological Chemistry. Sep, 2006 | Pubmed ID: 16887794 Emerging Roles of Pseudokinases Trends in Cell Biology. Sep, 2006 | Pubmed ID: 16879967 LKB1-dependent Signaling Pathways Annual Review of Biochemistry. 2006 | Pubmed ID: 16756488 Functional Interactions of the SPAK/OSR1 Kinases with Their Upstream Activator WNK1 and Downstream Substrate NKCC1 The Biochemical Journal. Jul, 2006 | Pubmed ID: 16669787 The Ubiquitin-associated Domain of AMPK-related Kinases Regulates Conformation and LKB1-mediated Phosphorylation and Activation The Biochemical Journal. Mar, 2006 | Pubmed ID: 16396636 Deficiency of LKB1 in Heart Prevents Ischemia-mediated Activation of AMPKalpha2 but Not AMPKalpha1 American Journal of Physiology. Endocrinology and Metabolism. May, 2006 | Pubmed ID: 16332922 14-3-3 Cooperates with LKB1 to Regulate the Activity and Localization of QSK and SIK Journal of Cell Science. Dec, 2005 | Pubmed ID: 16306228 Hypomorphic Mutation of PDK1 Suppresses Tumorigenesis in PTEN(+/-) Mice Current Biology : CB. Oct, 2005 | Pubmed ID: 16243031 In Vivo Role of the Phosphate Groove of PDK1 Defined by Knockin Mutation Journal of Cell Science. Nov, 2005 | Pubmed ID: 16219676 Analysis of Hepatic Gene Transcription in Mice Expressing Insulin-insensitive GSK3 The Biochemical Journal. Dec, 2005 | Pubmed ID: 16176184 The WNK1 and WNK4 Protein Kinases That Are Mutated in Gordon's Hypertension Syndrome Phosphorylate and Activate SPAK and OSR1 Protein Kinases The Biochemical Journal. Oct, 2005 | Pubmed ID: 16083423 Role of the PDK1-PKB-GSK3 Pathway in Regulating Glycogen Synthase and Glucose Uptake in the Heart FEBS Letters. Jul, 2005 | Pubmed ID: 15961082 Deficiency of LKB1 in Skeletal Muscle Prevents AMPK Activation and Glucose Uptake During Contraction The EMBO Journal. May, 2005 | Pubmed ID: 15889149 Metformin and Reduced Risk of Cancer in Diabetic Patients BMJ (Clinical Research Ed.). Jun, 2005 | Pubmed ID: 15849206 Regulation of Akt/PKB Ser473 Phosphorylation Molecular Cell. Apr, 2005 | Pubmed ID: 15837416 Role That Phosphorylation of GSK3 Plays in Insulin and Wnt Signalling Defined by Knockin Analysis The EMBO Journal. Apr, 2005 | Pubmed ID: 15791206 Role of T-loop Phosphorylation in PDK1 Activation, Stability, and Substrate Binding The Journal of Biological Chemistry. May, 2005 | Pubmed ID: 15741170 Identification of the Sucrose Non-fermenting Related Kinase SNRK, As a Novel LKB1 Substrate FEBS Letters. Feb, 2005 | Pubmed ID: 15733851 Crystal Structure of the PTPL1/FAP-1 Human Tyrosine Phosphatase Mutated in Colorectal Cancer: Evidence for a Second Phosphotyrosine Substrate Recognition Pocket The Journal of Biological Chemistry. Mar, 2005 | Pubmed ID: 15611135 Deficiency of PDK1 in Liver Results in Glucose Intolerance, Impairment of Insulin-regulated Gene Expression and Liver Failure The Biochemical Journal. Feb, 2005 | Pubmed ID: 15554902 Analysis of the LKB1-STRAD-MO25 Complex Journal of Cell Science. Dec, 2004 | Pubmed ID: 15561763 Structural Insights into the Regulation of PDK1 by Phosphoinositides and Inositol Phosphates The EMBO Journal. Oct, 2004 | Pubmed ID: 15457207 Cancer, Oncogenes and Signal Transduction Genome Biology. 2004 | Pubmed ID: 15239827 PDK1, the Master Regulator of AGC Kinase Signal Transduction Seminars in Cell & Developmental Biology. Apr, 2004 | Pubmed ID: 15209375 The in Vivo Role of PtdIns(3,4,5)P3 Binding to PDK1 PH Domain Defined by Knockin Mutation The EMBO Journal. May, 2004 | Pubmed ID: 15116068 Activity of LKB1 and AMPK-related Kinases in Skeletal Muscle: Effects of Contraction, Phenformin, and AICAR American Journal of Physiology. Endocrinology and Metabolism. Aug, 2004 | Pubmed ID: 15068958 LKB1 is a Master Kinase That Activates 13 Kinases of the AMPK Subfamily, Including MARK/PAR-1 The EMBO Journal. Feb, 2004 | Pubmed ID: 14976552 Interactions of LY333531 and Other Bisindolyl Maleimide Inhibitors with PDK1 Structure (London, England : 1993). Feb, 2004 | Pubmed ID: 14962382 Crystal Structure of MO25 Alpha in Complex with the C Terminus of the Pseudo Kinase STE20-related Adaptor Nature Structural & Molecular Biology. Feb, 2004 | Pubmed ID: 14730349 WNK1, the Kinase Mutated in an Inherited High-blood-pressure Syndrome, is a Novel PKB (protein Kinase B)/Akt Substrate The Biochemical Journal. Feb, 2004 | Pubmed ID: 14611643 MO25alpha/beta Interact with STRADalpha/beta Enhancing Their Ability to Bind, Activate and Localize LKB1 in the Cytoplasm The EMBO Journal. Oct, 2003 | Pubmed ID: 14517248 Interaction of the Protein Tyrosine Phosphatase PTPL1 with the PtdIns(3,4)P2-binding Adaptor Protein TAPP1 The Biochemical Journal. Dec, 2003 | Pubmed ID: 14516276 Complexes Between the LKB1 Tumor Suppressor, STRAD Alpha/beta and MO25 Alpha/beta Are Upstream Kinases in the AMP-activated Protein Kinase Cascade Journal of Biology. 2003 | Pubmed ID: 14511394 Deficiency of PDK1 in Cardiac Muscle Results in Heart Failure and Increased Sensitivity to Hypoxia The EMBO Journal. Sep, 2003 | Pubmed ID: 12970179 Binding of Phosphatidylinositol 3,4,5-trisphosphate to the Pleckstrin Homology Domain of Protein Kinase B Induces a Conformational Change The Biochemical Journal. Nov, 2003 | Pubmed ID: 12964941 In Vivo Role of the PIF-binding Docking Site of PDK1 Defined by Knock-in Mutation The EMBO Journal. Aug, 2003 | Pubmed ID: 12912918 Structural Basis for UCN-01 (7-hydroxystaurosporine) Specificity and PDK1 (3-phosphoinositide-dependent Protein Kinase-1) Inhibition The Biochemical Journal. Oct, 2003 | Pubmed ID: 12892559 Insulin-induced Drosophila S6 Kinase Activation Requires Phosphoinositide 3-kinase and Protein Kinase B The Biochemical Journal. Sep, 2003 | Pubmed ID: 12841848 LKB1, a Protein Kinase Regulating Cell Proliferation and Polarity FEBS Letters. Jul, 2003 | Pubmed ID: 12829253 Heat-shock Protein 90 and Cdc37 Interact with LKB1 and Regulate Its Stability The Biochemical Journal. Mar, 2003 | Pubmed ID: 12489981 Bi-functional, Substrate Mimicking RNA Inhibits MSK1-mediated CAMP-response Element-binding Protein Phosphorylation and Reveals Magnesium Ion-dependent Conformational Changes of the Kinase The Journal of Biological Chemistry. Nov, 2002 | Pubmed ID: 12235136 Ionizing Radiation Induces Ataxia Telangiectasia Mutated Kinase (ATM)-mediated Phosphorylation of LKB1/STK11 at Thr-366 The Biochemical Journal. Dec, 2002 | Pubmed ID: 12234250 High Resolution Crystal Structure of the Human PDK1 Catalytic Domain Defines the Regulatory Phosphopeptide Docking Site The EMBO Journal. Aug, 2002 | Pubmed ID: 12169624 Essential Role of PDK1 in Regulating Cell Size and Development in Mice The EMBO Journal. Jul, 2002 | Pubmed ID: 12110585 Molecular Basis for the Substrate Specificity of NIMA-related Kinase-6 (NEK6). Evidence That NEK6 Does Not Phosphorylate the Hydrophobic Motif of Ribosomal S6 Protein Kinase and Serum- and Glucocorticoid-induced Protein Kinase in Vivo The Journal of Biological Chemistry. Aug, 2002 | Pubmed ID: 12023960 The Insulin Signalling Pathway Current Biology : CB. Apr, 2002 | Pubmed ID: 11937037 Identification and Characterization of Four Novel Phosphorylation Sites (Ser31, Ser325, Thr336 and Thr366) on LKB1/STK11, the Protein Kinase Mutated in Peutz-Jeghers Cancer Syndrome The Biochemical Journal. Mar, 2002 | Pubmed ID: 11853558 Evidence That the Tandem-pleckstrin-homology-domain-containing Protein TAPP1 Interacts with Ptd(3,4)P2 and the Multi-PDZ-domain-containing Protein MUPP1 in Vivo The Biochemical Journal. Feb, 2002 | Pubmed ID: 11802782 The PIF-binding Pocket in PDK1 is Essential for Activation of S6K and SGK, but Not PKB The EMBO Journal. Aug, 2001 | Pubmed ID: 11500365 Regulation of Elongation Factor 2 Kinase by P90(RSK1) and P70 S6 Kinase The EMBO Journal. Aug, 2001 | Pubmed ID: 11500364 Discovery of PDK1, One of the Missing Links in Insulin Signal Transduction. Colworth Medal Lecture Biochemical Society Transactions. May, 2001 | Pubmed ID: 11356119 Identification of a Pocket in the PDK1 Kinase Domain That Interacts with PIF and the C-terminal Residues of PKA The EMBO Journal. Mar, 2000 | Pubmed ID: 10698939 Menselijke perifere bloed neutrofiele isolatie voor het ondervragen van de Parkinson Geassocieerde LRRK2 Kinase Pathway door de beoordeling van Rab10 fosforylatie Ying Fan*1, Francesca Tonelli*1, Shalini Padmanabhan2, Marco A.S. Baptista2, Lindsey Riley2, Danielle Smith3, Connie Marras4, Andrew Howden5, Dario R. Alessi1, Esther Sammler1,6 1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee JoVE 58956 생화학
Menselijke perifere bloed neutrofiele isolatie voor het ondervragen van de Parkinson Geassocieerde LRRK2 Kinase Pathway door de beoordeling van Rab10 fosforylatie Ying Fan*1, Francesca Tonelli*1, Shalini Padmanabhan2, Marco A.S. Baptista2, Lindsey Riley2, Danielle Smith3, Connie Marras4, Andrew Howden5, Dario R. Alessi1, Esther Sammler1,6 1MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2The Michael J. Fox Foundation for Parkinson's Research, 3Department of Medical and Molecular Genetics, Indiana University School of Medicine, 4Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto, 5Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee, 6Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee JoVE 58956 생화학