George M. Church Department of Genetics Harvard Medical School Biography Publications Institution JoVE Articles George M. Church has not added a biography. If you are George M. Church and would like to personalize this page please email our Author Liaison for assistance. Publications Predispositional Genome Sequencing in Healthy Adults: Design, Participant Characteristics, and Early Outcomes of the PeopleSeq Consortium Genome Medicine. Feb, 2019 | Pubmed ID: 30808425 REST and Neural Gene Network Dysregulation in IPSC Models of Alzheimer's Disease Cell Reports. Jan, 2019 | Pubmed ID: 30699343 Significant Abundance of Cis Configurations of Coding Variants in Diploid Human Genomes Nucleic Acids Research. Jan, 2019 | Pubmed ID: 30698752 Crystal Structure of the Redβ C-terminal Domain in Complex with λ Exonuclease Reveals an Unexpected Homology with λ Orf and an Interaction with Escherichia Coli Single Stranded DNA Binding Protein Nucleic Acids Research. Feb, 2019 | Pubmed ID: 30624736 CRISPR-C: Circularization of Genes and Chromosome by CRISPR in Human Cells Nucleic Acids Research. Dec, 2018 | Pubmed ID: 30551175 Characterizing Posttranslational Modifications in Prokaryotic Metabolism Using a Multiscale Workflow Proceedings of the National Academy of Sciences of the United States of America. 10, 2018 | Pubmed ID: 30301795 Establishing a Cell-Free Vibrio Natriegens Expression System ACS Synthetic Biology. Oct, 2018 | Pubmed ID: 30160938 Developmental Barcoding of Whole Mouse Via Homing CRISPR Science (New York, N.Y.). 08, 2018 | Pubmed ID: 30093604 Inter-homologue Repair in Fertilized Human Eggs? Nature. 08, 2018 | Pubmed ID: 30089924 Biosensor Libraries Harness Large Classes of Binding Domains for Construction of Allosteric Transcriptional Regulators Nature Communications. Aug, 2018 | Pubmed ID: 30082754 An Enhanced CRISPR Repressor for Targeted Mammalian Gene Regulation Nature Methods. Aug, 2018 | Pubmed ID: 30013045 Directed Differentiation of Human Induced Pluripotent Stem Cells into Mature Kidney Podocytes and Establishment of a Glomerulus Chip Nature Protocols. Jul, 2018 | Pubmed ID: 29995874 Enhanced Bacterial Immunity and Mammalian Genome Editing Via RNA-Polymerase-Mediated Dislodging of Cas9 from Double-Strand DNA Breaks Molecular Cell. Jul, 2018 | Pubmed ID: 29979968 Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach Current Protocols in Chemical Biology. 06, 2018 | Pubmed ID: 29927115 Current CRISPR Gene Drive Systems Are Likely to Be Highly Invasive in Wild Populations ELife. Jun, 2018 | Pubmed ID: 29916367 High-throughput Creation and Functional Profiling of DNA Sequence Variant Libraries Using CRISPR-Cas9 in Yeast Nature Biotechnology. Jul, 2018 | Pubmed ID: 29786095 Codon Usage of Highly Expressed Genes Affects Proteome-wide Translation Efficiency Proceedings of the National Academy of Sciences of the United States of America. 05, 2018 | Pubmed ID: 29735666 High-resolution Mapping of DNA Polymerase Fidelity Using Nucleotide Imbalances and Next-generation Sequencing Nucleic Acids Research. Jul, 2018 | Pubmed ID: 29718339 Simultaneous Discovery of Cell-Free DNA and the Nucleosome Ladder Genetics. May, 2018 | Pubmed ID: 29716984 An Integrated Genome-wide CRISPRa Approach to Functionalize LncRNAs in Drug Resistance Cell. Apr, 2018 | Pubmed ID: 29677511 Enabling Multiplexed Testing of Pooled Donor Cells Through Whole-genome Sequencing Genome Medicine. 04, 2018 | Pubmed ID: 29673390 Response to "Unexpected Mutations After CRISPR-Cas9 Editing in Vivo" Nature Methods. Apr, 2018 | Pubmed ID: 29600989 Precise Cas9 Targeting Enables Genomic Mutation Prevention Proceedings of the National Academy of Sciences of the United States of America. 04, 2018 | Pubmed ID: 29555762 Adaptive Evolution of Genomically Recoded Proceedings of the National Academy of Sciences of the United States of America. 03, 2018 | Pubmed ID: 29440500 Spontaneous CRISPR Loci Generation in Vivo by Non-canonical Spacer Integration Nature Microbiology. Mar, 2018 | Pubmed ID: 29379209 From Designing the Molecules of Life to Designing Life: Future Applications Derived from Advances in DNA Technologies Angewandte Chemie (International Ed. in English). Apr, 2018 | Pubmed ID: 29316123 Engineering Posttranslational Proofreading to Discriminate Nonstandard Amino Acids Proceedings of the National Academy of Sciences of the United States of America. 01, 2018 | Pubmed ID: 29301968 The Future of Multiplexed Eukaryotic Genome Engineering ACS Chemical Biology. 02, 2018 | Pubmed ID: 29241002 Efficient in Situ Barcode Sequencing Using Padlock Probe-based BaristaSeq Nucleic Acids Research. Feb, 2018 | Pubmed ID: 29190363 Heterologous Erythromycin Production Across Strain and Plasmid Construction Biotechnology Progress. Jan, 2018 | Pubmed ID: 28960932 A CRISPR-Cas9-based Gene Drive Platform for Genetic Interaction Analysis in Candida Albicans Nature Microbiology. Oct, 2017 | Pubmed ID: 29062088 Mature Induced-pluripotent-stem-cell-derived Human Podocytes Reconstitute Kidney Glomerular-capillary-wall Function on a Chip Nature Biomedical Engineering. 2017 | Pubmed ID: 29038743 DNA Sequencing at 40: Past, Present and Future Nature. 10, 2017 | Pubmed ID: 29019985 Rapid Sequential in Situ Multiplexing with DNA Exchange Imaging in Neuronal Cells and Tissues Nano Letters. 10, 2017 | Pubmed ID: 28933153 A Systematic Comparison of Error Correction Enzymes by Next-generation Sequencing Nucleic Acids Research. Sep, 2017 | Pubmed ID: 28911123 Beyond Editing to Writing Large Genomes Nature Reviews. Genetics. 12, 2017 | Pubmed ID: 28852223 Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828661 Extracellular Vesicle Isolation and Analysis by Western Blotting Methods in Molecular Biology (Clifton, N.J.). 2017 | Pubmed ID: 28828654 Inactivation of Porcine Endogenous Retrovirus in Pigs Using CRISPR-Cas9 Science (New York, N.Y.). 09, 2017 | Pubmed ID: 28798043 Rates, Distribution and Implications of Postzygotic Mosaic Mutations in Autism Spectrum Disorder Nature Neuroscience. 09, 2017 | Pubmed ID: 28714951 Dissecting Limiting Factors of the Protein Synthesis Using Recombinant Elements (PURE) System Translation (Austin, Tex.). 2017 | Pubmed ID: 28702280 CRISPR-Cas Encoding of a Digital Movie into the Genomes of a Population of Living Bacteria Nature. 07, 2017 | Pubmed ID: 28700573 The Promise of Organ and Tissue Preservation to Transform Medicine Nature Biotechnology. 06, 2017 | Pubmed ID: 28591112 Scalable Device for Automated Microbial Electroporation in a Digital Microfluidic Platform ACS Synthetic Biology. 09, 2017 | Pubmed ID: 28569062 Millstone: Software for Multiplex Microbial Genome Analysis and Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545559 Optimizing Complex Phenotypes Through Model-guided Multiplex Genome Engineering Genome Biology. 05, 2017 | Pubmed ID: 28545477 Addressing the Ethical Issues Raised by Synthetic Human Entities with Embryo-like Features ELife. 03, 2017 | Pubmed ID: 28494856 Nucleotide-time Alignment for Molecular Recorders PLoS Computational Biology. May, 2017 | Pubmed ID: 28459860 Evolutionary Dynamics of CRISPR Gene Drives Science Advances. Apr, 2017 | Pubmed ID: 28435878 An Unbiased Index to Quantify Participant's Phenotypic Contribution to an Open-access Cohort Scientific Reports. 04, 2017 | Pubmed ID: 28387241 Cogenerating Synthetic Parts Toward a Self-Replicating System ACS Synthetic Biology. 07, 2017 | Pubmed ID: 28330337 Programmable Transcriptional Repression in Mycobacteria Using an Orthogonal CRISPR Interference Platform Nature Microbiology. Feb, 2017 | Pubmed ID: 28165460 SgRNA Scorer 2.0: A Species-Independent Model To Predict CRISPR/Cas9 Activity ACS Synthetic Biology. 05, 2017 | Pubmed ID: 28146356 Cushing's Syndrome Mutant PKA Exhibits Altered Substrate Specificity FEBS Letters. 02, 2017 | Pubmed ID: 28100013 Efficient, Footprint-free Human IPSC Genome Editing by Consolidation of Cas9/CRISPR and PiggyBac Technologies Nature Protocols. Jan, 2017 | Pubmed ID: 27929521 Rapidly Evolving Homing CRISPR Barcodes Nature Methods. 02, 2017 | Pubmed ID: 27918539 An Ethnically Relevant Consensus Korean Reference Genome is a Step Towards Personal Reference Genomes Nature Communications. 11, 2016 | Pubmed ID: 27882922 Complete Mitochondrial Genomes of Living and Extinct Pigeons Revise the Timing of the Columbiform Radiation BMC Evolutionary Biology. 10, 2016 | Pubmed ID: 27782796 Design and Characterization of a Nanopore-coupled Polymerase for Single-molecule DNA Sequencing by Synthesis on an Electrode Array Proceedings of the National Academy of Sciences of the United States of America. 11, 2016 | Pubmed ID: 27729524 The Whole Genome Sequences and Experimentally Phased Haplotypes of over 100 Personal Genomes GigaScience. Oct, 2016 | Pubmed ID: 27724973 Emergent Rules for Codon Choice Elucidated by Editing Rare Arginine Codons in Escherichia Coli Proceedings of the National Academy of Sciences of the United States of America. 09, 2016 | Pubmed ID: 27601680 A Multifunctional AAV-CRISPR-Cas9 and Its Host Response Nature Methods. 10, 2016 | Pubmed ID: 27595405 CRISPR-Cas9 System: Opportunities and Concerns Clinical Chemistry. 10, 2016 | Pubmed ID: 27551006 Design, Synthesis, and Testing Toward a 57-codon Genome Science (New York, N.Y.). Aug, 2016 | Pubmed ID: 27540174 Nanoscale Imaging of RNA with Expansion Microscopy Nature Methods. 08, 2016 | Pubmed ID: 27376770 Molecular Recordings by Directed CRISPR Spacer Acquisition Science (New York, N.Y.). Jul, 2016 | Pubmed ID: 27284167 Extensive Sequencing of Seven Human Genomes to Characterize Benchmark Reference Materials Scientific Data. Jun, 2016 | Pubmed ID: 27271295 Characterization of Cas9-Guide RNA Orthologs Cold Spring Harbor Protocols. 05, 2016 | Pubmed ID: 27140923 Characterizing Cas9 Protospacer-Adjacent Motifs with High-Throughput Sequencing of Library Depletion Experiments Cold Spring Harbor Protocols. 05, 2016 | Pubmed ID: 27140916 Real-time Single-molecule Electronic DNA Sequencing by Synthesis Using Polymer-tagged Nucleotides on a Nanopore Array Proceedings of the National Academy of Sciences of the United States of America. May, 2016 | Pubmed ID: 27091962 Performance of Optimized Noncanonical Amino Acid Mutagenesis Systems in the Absence of Release Factor 1 Molecular BioSystems. 05, 2016 | Pubmed ID: 27027374 Biosensor-based Engineering of Biosynthetic Pathways Current Opinion in Biotechnology. 12, 2016 | Pubmed ID: 26998575 Simulating Serial-Target Antibacterial Drug Synergies Using Flux Balance Analysis PloS One. 2016 | Pubmed ID: 26821252 In Vivo Gene Editing in Dystrophic Mouse Muscle and Muscle Stem Cells Science (New York, N.Y.). Jan, 2016 | Pubmed ID: 26721686 Engineering an Allosteric Transcription Factor to Respond to New Ligands Nature Methods. Feb, 2016 | Pubmed ID: 26689263 Efficient Reassignment of a Frequent Serine Codon in Wild-Type Escherichia Coli ACS Synthetic Biology. Feb, 2016 | Pubmed ID: 26544153 Precision Chemistry for Precision Medicine ACS Central Science. Mar, 2015 | Pubmed ID: 27162939 Crispr-mediated Gene Targeting of Human Induced Pluripotent Stem Cells Current Protocols in Stem Cell Biology. 2015 | Pubmed ID: 26949444 A General Strategy to Construct Small Molecule Biosensors in Eukaryotes ELife. Dec, 2015 | Pubmed ID: 26714111 Bioart Trends in Biotechnology. Dec, 2015 | Pubmed ID: 26617334 Safeguarding CRISPR-Cas9 Gene Drives in Yeast Nature Biotechnology. Dec, 2015 | Pubmed ID: 26571100 Neutralizing Antibodies Against West Nile Virus Identified Directly from Human B Cells by Single-cell Analysis and Next Generation Sequencing Integrative Biology : Quantitative Biosciences from Nano to Macro. Dec, 2015 | Pubmed ID: 26481611 A National Network of Neurotechnology Centers for the BRAIN Initiative Neuron. Nov, 2015 | Pubmed ID: 26481036 BIOSAFETY. Safeguarding Gene Drive Experiments in the Laboratory Science (New York, N.Y.). Aug, 2015 | Pubmed ID: 26229113 Puzzle Imaging: Using Large-Scale Dimensionality Reduction Algorithms for Localization PloS One. 2015 | Pubmed ID: 26192446 Unraveling CRISPR-Cas9 Genome Engineering Parameters Via a Library-on-library Approach Nature Methods. Sep, 2015 | Pubmed ID: 26167643 Synthetic Biosensors for Precise Gene Control and Real-time Monitoring of Metabolites Nucleic Acids Research. Sep, 2015 | Pubmed ID: 26152303 Genome and Transcriptome of Clostridium Phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels PloS One. 2015 | Pubmed ID: 26035711 Highly Efficient Cas9-mediated Transcriptional Programming Nature Methods. Apr, 2015 | Pubmed ID: 25730490 Fluorescent in Situ Sequencing (FISSEQ) of RNA for Gene Expression Profiling in Intact Cells and Tissues Nature Protocols. Mar, 2015 | Pubmed ID: 25675209 Biocontainment of Genetically Modified Organisms by Synthetic Protein Design Nature. Feb, 2015 | Pubmed ID: 25607366 Insights into the Evolution of Longevity from the Bowhead Whale Genome Cell Reports. Jan, 2015 | Pubmed ID: 25565328 Complete Genome Sequences of T4-Like Bacteriophages RB3, RB5, RB6, RB7, RB9, RB10, RB27, RB33, RB55, RB59, and RB68 Genome Announcements. Jan, 2015 | Pubmed ID: 25555735 Accurate Whole Genome Sequencing As the Ultimate Genetic Test Clinical Chemistry. Jan, 2015 | Pubmed ID: 25479756 Multi-kilobase Homozygous Targeted Gene Replacement in Human Induced Pluripotent Stem Cells Nucleic Acids Research. Feb, 2015 | Pubmed ID: 25414332 Direct Mutagenesis of Thousands of Genomic Targets Using Microarray-derived Oligonucleotides ACS Synthetic Biology. Jan, 2015 | Pubmed ID: 24856730 Spatial Information in Large-scale Neural Recordings Frontiers in Computational Neuroscience. 2014 | Pubmed ID: 25653613 Evolution-guided Optimization of Biosynthetic Pathways Proceedings of the National Academy of Sciences of the United States of America. Dec, 2014 | Pubmed ID: 25453111 Multiple Haplotype-resolved Genomes Reveal Population Patterns of Gene and Protein Diplotypes Nature Communications. Nov, 2014 | Pubmed ID: 25424553 Rapid Neurogenesis Through Transcriptional Activation in Human Stem Cells Molecular Systems Biology. Nov, 2014 | Pubmed ID: 25403753 Genome Editing in Human Stem Cells Methods in Enzymology. 2014 | Pubmed ID: 25398338 Engineering Allostery Trends in Genetics : TIG. Dec, 2014 | Pubmed ID: 25306102 Multiplex Single-molecule Interaction Profiling of DNA-barcoded Proteins Nature. Nov, 2014 | Pubmed ID: 25252978 Improved Cell-free RNA and Protein Synthesis System PloS One. 2014 | Pubmed ID: 25180701 The Naked Mole Rat Genome Resource: Facilitating Analyses of Cancer and Longevity-related Adaptations Bioinformatics (Oxford, England). Dec, 2014 | Pubmed ID: 25172923 Concerning RNA-guided Gene Drives for the Alteration of Wild Populations ELife. 07, 2014 | Pubmed ID: 25035423 Genome Editing Assessment Using CRISPR Genome Analyzer (CRISPR-GA) Bioinformatics (Oxford, England). Oct, 2014 | Pubmed ID: 24990609 CRISPR/Cas9-Directed Genome Editing of Cultured Cells Current Protocols in Molecular Biology. Jul, 2014 | Pubmed ID: 24984853 CRISPR/Cas9-mediated Phage Resistance is Not Impeded by the DNA Modifications of Phage T4 PloS One. 2014 | Pubmed ID: 24886988 CHOPCHOP: a CRISPR/Cas9 and TALEN Web Tool for Genome Editing Nucleic Acids Research. Jul, 2014 | Pubmed ID: 24861617 Modeling the Mitochondrial Cardiomyopathy of Barth Syndrome with Induced Pluripotent Stem Cell and Heart-on-chip Technologies Nature Medicine. Jun, 2014 | Pubmed ID: 24813252 Fungal Lysis by a Soil Bacterium Fermenting Cellulose Environmental Microbiology. May, 2014 | Pubmed ID: 24798076 Large-scale De Novo DNA Synthesis: Technologies and Applications Nature Methods. May, 2014 | Pubmed ID: 24781323 Syntrophic Exchange in Synthetic Microbial Communities Proceedings of the National Academy of Sciences of the United States of America. May, 2014 | Pubmed ID: 24778240 Harvard Personal Genome Project: Lessons from Participatory Public Research Genome Medicine. Feb, 2014 | Pubmed ID: 24713084 High-resolution Antibody Dynamics of Vaccine-induced Immune Responses Proceedings of the National Academy of Sciences of the United States of America. Apr, 2014 | Pubmed ID: 24639495 Realizing the Potential of Synthetic Biology Nature Reviews. Molecular Cell Biology. 04, 2014 | Pubmed ID: 24622617 Raw Data: Access to Inaccuracy--response Science (New York, N.Y.). Feb, 2014 | Pubmed ID: 24578564 Highly Multiplexed Subcellular RNA Sequencing in Situ Science (New York, N.Y.). Mar, 2014 | Pubmed ID: 24578530 Recoding the Genetic Code with Selenocysteine Angewandte Chemie (International Ed. in English). Jan, 2014 | Pubmed ID: 24511637 Information Access. Raw Personal Data: Providing Access Science (New York, N.Y.). Jan, 2014 | Pubmed ID: 24458627 Rational Optimization of TolC As a Powerful Dual Selectable Marker for Genome Engineering Nucleic Acids Research. Apr, 2014 | Pubmed ID: 24452804 Integrating B Cell Lineage Information into Statistical Tests for Detecting Selection in Ig Sequences Journal of Immunology (Baltimore, Md. : 1950). Feb, 2014 | Pubmed ID: 24376267 The Role of Replicates for Error Mitigation in Next-generation Sequencing Nature Reviews. Genetics. 01, 2014 | Pubmed ID: 24322726 Natural Reassignment of CUU and CUA Sense Codons to Alanine in Ashbya Mitochondria Nucleic Acids Research. Jan, 2014 | Pubmed ID: 24049072 Next-generation Carrier Screening Genetics in Medicine : Official Journal of the American College of Medical Genetics. Feb, 2014 | Pubmed ID: 23765052 The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies PLoS Pathogens. 2013 | Pubmed ID: 24278016 BRAIN: Innovative Neurotechnologies for Imaging and Therapeutics Dialogues in Clinical Neuroscience. Sep, 2013 | Pubmed ID: 24174897 Deciphering the Functions and Regulation of Brain-enriched A-to-I RNA Editing Nature Neuroscience. Nov, 2013 | Pubmed ID: 24165678 Yeast Oligo-mediated Genome Engineering (YOGE) ACS Synthetic Biology. Dec, 2013 | Pubmed ID: 24160921 Genomically Recoded Organisms Expand Biological Functions Science (New York, N.Y.). Oct, 2013 | Pubmed ID: 24136966 Cas9 As a Versatile Tool for Engineering Biology Nature Methods. Oct, 2013 | Pubmed ID: 24076990 Orthogonal Cas9 Proteins for RNA-guided Gene Regulation and Editing Nature Methods. Nov, 2013 | Pubmed ID: 24076762 Mutants of Cre Recombinase with Improved Accuracy Nature Communications. 2013 | Pubmed ID: 24056590 Heritable Custom Genomic Modifications in Caenorhabditis Elegans Via a CRISPR-Cas9 System Genetics. Nov, 2013 | Pubmed ID: 23979579 Multiplex and Homologous Recombination-mediated Genome Editing in Arabidopsis and Nicotiana Benthamiana Using Guide RNA and Cas9 Nature Biotechnology. Aug, 2013 | Pubmed ID: 23929339 Composability of Regulatory Sequences Controlling Transcription and Translation in Escherichia Coli Proceedings of the National Academy of Sciences of the United States of America. Aug, 2013 | Pubmed ID: 23924614 CAS9 Transcriptional Activators for Target Specificity Screening and Paired Nickases for Cooperative Genome Engineering Nature Biotechnology. Sep, 2013 | Pubmed ID: 23907171 Optical Control of Mammalian Endogenous Transcription and Epigenetic States Nature. Aug, 2013 | Pubmed ID: 23877069 Quantification of MicroRNA Expression with Next-generation Sequencing Current Protocols in Molecular Biology. Jul, 2013 | Pubmed ID: 23821442 Heritable Genome Editing in C. Elegans Via a CRISPR-Cas9 System Nature Methods. Aug, 2013 | Pubmed ID: 23817069 In Vitro Integration of Ribosomal RNA Synthesis, Ribosome Assembly, and Translation Molecular Systems Biology. Jun, 2013 | Pubmed ID: 23799452 Nanotools for Neuroscience and Brain Activity Mapping ACS Nano. Mar, 2013 | Pubmed ID: 23514423 Barcoding Cells Using Cell-surface Programmable DNA-binding Domains Nature Methods. May, 2013 | Pubmed ID: 23503053 PhIP-Seq Characterization of Autoantibodies from Patients with Multiple Sclerosis, Type 1 Diabetes and Rheumatoid Arthritis Journal of Autoimmunity. Jun, 2013 | Pubmed ID: 23497938 Neuroscience. The Brain Activity Map Science (New York, N.Y.). Mar, 2013 | Pubmed ID: 23470729 Genome Engineering in Saccharomyces Cerevisiae Using CRISPR-Cas Systems Nucleic Acids Research. Apr, 2013 | Pubmed ID: 23460208 Redirector: Designing Cell Factories by Reconstructing the Metabolic Objective PLoS Computational Biology. 2013 | Pubmed ID: 23341769 Reading and Writing Omes Molecular Systems Biology. 2013 | Pubmed ID: 23340848 An Analysis and Validation Pipeline for Large-scale RNAi-based Screens Scientific Reports. 2013 | Pubmed ID: 23326633 RNA-guided Human Genome Engineering Via Cas9 Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287722 Gene Assembly from Chip-Synthesized Oligonucleotides Current Protocols in Chemical Biology. Mar, 2012 | Pubmed ID: 25077042 Using Bacteria to Determine Protein Kinase Specificity and Predict Target Substrates PloS One. 2012 | Pubmed ID: 23300758 Submicrometre Geometrically Encoded Fluorescent Barcodes Self-assembled from DNA Nature Chemistry. Oct, 2012 | Pubmed ID: 23000997 Improving Lambda Red Genome Engineering in Escherichia Coli Via Rational Removal of Endogenous Nucleases PloS One. 2012 | Pubmed ID: 22957093 Next-generation Digital Information Storage in DNA Science (New York, N.Y.). Sep, 2012 | Pubmed ID: 22903519 How Accurate Can Genetic Predictions Be? BMC Genomics. Jul, 2012 | Pubmed ID: 22827772 A Public Resource Facilitating Clinical Use of Genomes Proceedings of the National Academy of Sciences of the United States of America. Jul, 2012 | Pubmed ID: 22797899 Accurate Whole-genome Sequencing and Haplotyping from 10 to 20 Human Cells Nature. Jul, 2012 | Pubmed ID: 22785314 Iterative Capped Assembly: Rapid and Scalable Synthesis of Repeat-module DNA Such As TAL Effectors from Individual Monomers Nucleic Acids Research. Aug, 2012 | Pubmed ID: 22740649 Multiplexed in Vivo His-tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis ACS Synthetic Biology. Feb, 2012 | Pubmed ID: 22737598 The Brain Activity Map Project and the Challenge of Functional Connectomics Neuron. Jun, 2012 | Pubmed ID: 22726828 A BioBrick Compatible Strategy for Genetic Modification of Plants Journal of Biological Engineering. 2012 | Pubmed ID: 22716313 Enhanced Multiplex Genome Engineering Through Co-operative Oligonucleotide Co-selection Nucleic Acids Research. Sep, 2012 | Pubmed ID: 22638574 Stable Gene Targeting in Human Cells Using Single-strand Oligonucleotides with Modified Bases PloS One. 2012 | Pubmed ID: 22615794 Systematic Identification of Edited MicroRNAs in the Human Brain Genome Research. Aug, 2012 | Pubmed ID: 22499667 Genome-scale Promoter Engineering by Coselection MAGE Nature Methods. Jun, 2012 | Pubmed ID: 22484848 High-throughput Multiplex Sequencing of MiRNA Current Protocols in Human Genetics. Apr, 2012 | Pubmed ID: 22470142 Back to the Future: from Genome to Metabolome Human Mutation. May, 2012 | Pubmed ID: 22431014 Genomic Analysis of the Hydrocarbon-producing, Cellulolytic, Endophytic Fungus Ascocoryne Sarcoides PLoS Genetics. 2012 | Pubmed ID: 22396667 A Logic-gated Nanorobot for Targeted Transport of Molecular Payloads Science (New York, N.Y.). Feb, 2012 | Pubmed ID: 22344439 RNA Sequencing Reveals Differential Expression of Mitochondrial and Oxidation Reduction Genes in the Long-lived Naked Mole-rat when Compared to Mice PloS One. 2011 | Pubmed ID: 22073188 The Three-dimensional Architecture of a Bacterial Genome and Its Alteration by Genetic Perturbation Molecular Cell. Oct, 2011 | Pubmed ID: 22017872 Panel Discussion: Can There Be a Biomarker for Sleepiness? Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine. Oct, 2011 | Pubmed ID: 22003333 New Technologies for Integrating Genomic, Environmental and Trait Data Journal of Clinical Sleep Medicine : JCSM : Official Publication of the American Academy of Sleep Medicine. Oct, 2011 | Pubmed ID: 22003332 Phased Whole-genome Genetic Risk in a Family Quartet Using a Major Allele Reference Sequence PLoS Genetics. Sep, 2011 | Pubmed ID: 21935354 Precise Manipulation of Chromosomes in Vivo Enables Genome-wide Codon Replacement Science (New York, N.Y.). Jul, 2011 | Pubmed ID: 21764749 Barcoding Bias in High-throughput Multiplex Sequencing of MiRNA Genome Research. Sep, 2011 | Pubmed ID: 21750102 Multiplexed Genome Engineering and Genotyping Methods Applications for Synthetic Biology and Metabolic Engineering Methods in Enzymology. 2011 | Pubmed ID: 21601688 Proteome-wide Systems Analysis of a Cellulosic Biofuel-producing Microbe Molecular Systems Biology. Jan, 2011 | Pubmed ID: 21245846 Research, Collaboration, and Open Science Using Web 2.0 Journal of Microbiology & Biology Education. 2010 | Pubmed ID: 23653712 The Human Microbiome Harbors a Diverse Reservoir of Antibiotic Resistance Genes Virulence. Jul-Aug, 2010 | Pubmed ID: 21178459 Scalable Gene Synthesis by Selective Amplification of DNA Pools from High-fidelity Microchips Nature Biotechnology. Dec, 2010 | Pubmed ID: 21113165 Collection and Motif-based Prediction of Phosphorylation Sites in Human Viruses Science Signaling. Aug, 2010 | Pubmed ID: 20807955 Reprogramming of T Cells from Human Peripheral Blood Cell Stem Cell. Jul, 2010 | Pubmed ID: 20621044 A Survey of Genomic Traces Reveals a Common Sequencing Error, RNA Editing, and DNA Editing PLoS Genetics. May, 2010 | Pubmed ID: 20531933 A Functional Metagenomic Approach for Expanding the Synthetic Biology Toolbox for Biomass Conversion Molecular Systems Biology. Apr, 2010 | Pubmed ID: 20393580 Extensive Phosphorylation with Overlapping Specificity by Mycobacterium Tuberculosis Serine/threonine Protein Kinases Proceedings of the National Academy of Sciences of the United States of America. Apr, 2010 | Pubmed ID: 20368441 Generation of Functional Human Hepatic Endoderm from Human Induced Pluripotent Stem Cells Hepatology (Baltimore, Md.). Jan, 2010 | Pubmed ID: 19877180 A Robust Approach to Identifying Tissue-specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells PLoS Genetics. Nov, 2009 | Pubmed ID: 19911041 Targeted Gene Inactivation in Clostridium Phytofermentans Shows That Cellulose Degradation Requires the Family 9 Hydrolase Cphy3367 Molecular Microbiology. Dec, 2009 | Pubmed ID: 19775243 Evidence for Large Diversity in the Human Transcriptome Created by Alu RNA Editing Nucleic Acids Research. Nov, 2009 | Pubmed ID: 19740767 Functional Characterization of the Antibiotic Resistance Reservoir in the Human Microflora Science (New York, N.Y.). Aug, 2009 | Pubmed ID: 19713526 Large-scale Identification of Genetic Design Strategies Using Local Search Molecular Systems Biology. 2009 | Pubmed ID: 19690565 Programming Cells by Multiplex Genome Engineering and Accelerated Evolution Nature. Aug, 2009 | Pubmed ID: 19633652 The Scientific Foundation for Personal Genomics: Recommendations from a National Institutes of Health-Centers for Disease Control and Prevention Multidisciplinary Workshop Genetics in Medicine : Official Journal of the American College of Medical Genetics. Aug, 2009 | Pubmed ID: 19617843 A Highly Annotated Whole-genome Sequence of a Korean Individual Nature. Aug, 2009 | Pubmed ID: 19587683 Rapid Prototyping of 3D DNA-origami Shapes with CaDNAno Nucleic Acids Research. Aug, 2009 | Pubmed ID: 19531737 Multiplex Padlock Targeted Sequencing Reveals Human Hypermutable CpG Variations Genome Research. Sep, 2009 | Pubmed ID: 19525355 Genome-wide Identification of Human RNA Editing Sites by Parallel DNA Capturing and Sequencing Science (New York, N.Y.). May, 2009 | Pubmed ID: 19478186 Targeted and Genome-scale Strategies Reveal Gene-body Methylation Signatures in Human Cells Nature Biotechnology. Apr, 2009 | Pubmed ID: 19329998 Meta-analysis of Age-related Gene Expression Profiles Identifies Common Signatures of Aging Bioinformatics (Oxford, England). Apr, 2009 | Pubmed ID: 19189975 The Human Ageing Genomic Resources: Online Databases and Tools for Biogerontologists Aging Cell. Feb, 2009 | Pubmed ID: 18986374 Predicting Protein Post-translational Modifications Using Meta-analysis of Proteome Scale Data Sets Molecular & Cellular Proteomics : MCP. Feb, 2009 | Pubmed ID: 18974045 Efficient MicroRNA Capture and Bar-coding Via Enzymatic Oligonucleotide Adenylation Nature Methods. Sep, 2008 | Pubmed ID: 19160512 Model-driven Analysis of Experimentally Determined Growth Phenotypes for 465 Yeast Gene Deletion Mutants Under 16 Different Conditions Genome Biology. 2008 | Pubmed ID: 18808699 Hippocrates Revisited? Old Ideals and New Realities Genomic Medicine. Jan, 2008 | Pubmed ID: 18716915 Bacteria Subsisting on Antibiotics Science (New York, N.Y.). Apr, 2008 | Pubmed ID: 18388292 Gene Synthesis by Circular Assembly Amplification Nature Methods. Jan, 2008 | Pubmed ID: 18037891 Patterns and Implications of Gene Gain and Loss in the Evolution of Prochlorococcus PLoS Genetics. Dec, 2007 | Pubmed ID: 18159947 Multiplex Amplification of Large Sets of Human Exons Nature Methods. Nov, 2007 | Pubmed ID: 17934468 A Proposal to Sequence Genomes of Unique Interest for Research on Aging The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. Jun, 2007 | Pubmed ID: 17595413 Chromosomal Periodicity of Evolutionarily Conserved Gene Pairs Proceedings of the National Academy of Sciences of the United States of America. Jun, 2007 | Pubmed ID: 17563360 Analyses of Human-chimpanzee Orthologous Gene Pairs to Explore Evolutionary Hypotheses of Aging Mechanisms of Ageing and Development. May-Jun, 2007 | Pubmed ID: 17459455 An Analysis of the Relationship Between Metabolism, Developmental Schedules, and Longevity Using Phylogenetic Independent Contrasts The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences. Feb, 2007 | Pubmed ID: 17339640 Polony DNA Sequencing Current Protocols in Molecular Biology. Nov, 2006 | Pubmed ID: 18265387 Relationships Between P63 Binding, DNA Sequence, Transcription Activity, and Biological Function in Human Cells Molecular Cell. Nov, 2006 | Pubmed ID: 17188034 The Transition Between Transcriptional Initiation and Elongation in E. Coli is Highly Variable and Often Rate Limiting Molecular Cell. Dec, 2006 | Pubmed ID: 17157257 Global Gene Expression of Prochlorococcus Ecotypes in Response to Changes in Nitrogen Availability Molecular Systems Biology. 2006 | Pubmed ID: 17016519 Mammalian Ultraconserved Elements Are Strongly Depleted Among Segmental Duplications and Copy Number Variants Nature Genetics. Oct, 2006 | Pubmed ID: 16998490 Identifying Regulatory Mechanisms Using Individual Variation Reveals Key Role for Chromatin Modification Proceedings of the National Academy of Sciences of the United States of America. Sep, 2006 | Pubmed ID: 16968785 Towards Synthesis of a Minimal Cell Molecular Systems Biology. 2006 | Pubmed ID: 16924266 Sequencing Genomes from Single Cells by Polymerase Cloning Nature Biotechnology. Jun, 2006 | Pubmed ID: 16732271 Identifying Metabolic Enzymes with Multiple Types of Association Evidence BMC Bioinformatics. Mar, 2006 | Pubmed ID: 16571130 Long-range Polony Haplotyping of Individual Human Chromosome Molecules Nature Genetics. Mar, 2006 | Pubmed ID: 16493423 An Integrated Strategy for Analyzing the Unique Developmental Programs of Different Myoblast Subtypes PLoS Genetics. Feb, 2006 | Pubmed ID: 16482229 MapQuant: Open-source Software for Large-scale Protein Quantification Proteomics. Mar, 2006 | Pubmed ID: 16470651 Genomes for All Scientific American. Jan, 2006 | Pubmed ID: 16468433 Cells Discover Fire: Employing Reactive Oxygen Species in Development and Consequences for Aging Experimental Gerontology. Jan, 2006 | Pubmed ID: 16226003 From Systems Biology to Synthetic Biology Molecular Systems Biology. 2005 | Pubmed ID: 16729067 Expression Dynamics of a Cellular Metabolic Network Molecular Systems Biology. 2005 | Pubmed ID: 16729051 Genomic Analysis of LexA Binding Reveals the Permissive Nature of the Escherichia Coli Genome and Identifies Unconventional Target Sites Genes & Development. Nov, 2005 | Pubmed ID: 16264194 A Network of Transcriptionally Coordinated Functional Modules in Saccharomyces Cerevisiae Genome Research. Sep, 2005 | Pubmed ID: 16109970 Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome Science (New York, N.Y.). Sep, 2005 | Pubmed ID: 16081699 Genomes Optimize Reproduction: Aging As a Consequence of the Developmental Program Physiology (Bethesda, Md.). Aug, 2005 | Pubmed ID: 16024513 Molecular Weight Assessment of Proteins in Total Proteome Profiles Using 1D-PAGE and LC/MS/MS Proteome Science. Jun, 2005 | Pubmed ID: 15941491 Discovering Functional Transcription-factor Combinations in the Human Cell Cycle Genome Research. Jun, 2005 | Pubmed ID: 15930495 Preferred Analysis Methods for Affymetrix GeneChips Revealed by a Wholly Defined Control Dataset Genome Biology. 2005 | Pubmed ID: 15693945 Modular Epistasis in Yeast Metabolism Nature Genetics. Jan, 2005 | Pubmed ID: 15592468 Estimating and Improving Protein Interaction Error Rates Proceedings. IEEE Computational Systems Bioinformatics Conference. 2004 | Pubmed ID: 16448015 The Complete Genome and Proteome of Mycoplasma Mobile Genome Research. Aug, 2004 | Pubmed ID: 15289470 Filling Gaps in a Metabolic Network Using Expression Information Bioinformatics (Oxford, England). Aug, 2004 | Pubmed ID: 15262797 Preferred in Vivo Ubiquitination Sites Bioinformatics (Oxford, England). Dec, 2004 | Pubmed ID: 15256413 Prediction of Similarly Acting Cis-regulatory Modules by Subsequence Profiling and Comparative Genomics in Drosophila Melanogaster and D.pseudoobscura Bioinformatics (Oxford, England). Nov, 2004 | Pubmed ID: 15145800 Advanced Sequencing Technologies: Methods and Goals Nature Reviews. Genetics. May, 2004 | Pubmed ID: 15143316 Mathematical Models of Diffusion-constrained Polymerase Chain Reactions: Basis of High-throughput Nucleic Acid Assays and Simple Self-organizing Systems Journal of Theoretical Biology. May, 2004 | Pubmed ID: 15064081 A Motif Co-occurrence Approach for Genome-wide Prediction of Transcription-factor-binding Sites in Escherichia Coli Genome Research. Feb, 2004 | Pubmed ID: 14762058 Identification of Many MicroRNAs That Copurify with Polyribosomes in Mammalian Neurons Proceedings of the National Academy of Sciences of the United States of America. Jan, 2004 | Pubmed ID: 14691248 YMGV: a Cross-species Expression Data Mining Tool Nucleic Acids Research. Jan, 2004 | Pubmed ID: 14681424 The Amino-acid Mutational Spectrum of Human Genetic Disease Genome Biology. 2003 | Pubmed ID: 14611658 Parallel Competition Analysis of Saccharomyces Cerevisiae Strains Differing by a Single Base Using Polymerase Colonies Nucleic Acids Research. Aug, 2003 | Pubmed ID: 12888536 Digital Genotyping and Haplotyping with Polymerase Colonies Proceedings of the National Academy of Sciences of the United States of America. May, 2003 | Pubmed ID: 12730373 Global RNA Half-life Analysis in Escherichia Coli Reveals Positional Patterns of Transcript Degradation Genome Research. Feb, 2003 | Pubmed ID: 12566399 A Microarray-based Antibiotic Screen Identifies a Regulatory Role for Supercoiling in the Osmotic Stress Response of Escherichia Coli Genome Research. Feb, 2003 | Pubmed ID: 12566398 Genome-wide Co-occurrence of Promoter Elements Reveals a Cis-regulatory Cassette of RRNA Transcription Motifs in Saccharomyces Cerevisiae Genome Research. Nov, 2002 | Pubmed ID: 12421759 Analysis of Optimality in Natural and Perturbed Metabolic Networks Proceedings of the National Academy of Sciences of the United States of America. Nov, 2002 | Pubmed ID: 12415116 Escherichia Coli Gene Expression Responsive to Levels of the Response Regulator EvgA Journal of Bacteriology. Nov, 2002 | Pubmed ID: 12399493 Computational Discovery of Sense-antisense Transcription in the Human and Mouse Genomes Genome Biology. Aug, 2002 | Pubmed ID: 12225583 Genome-scale Metabolic Model of Helicobacter Pylori 26695 Journal of Bacteriology. Aug, 2002 | Pubmed ID: 12142428 Computation-based Discovery of Related Transcriptional Regulatory Modules and Motifs Using an Experimentally Validated Combinatorial Model Genome Research. Jul, 2002 | Pubmed ID: 12097338 Measuring Absolute Expression with Microarrays with a Calibrated Reference Sample and an Extended Signal Intensity Range Proceedings of the National Academy of Sciences of the United States of America. May, 2002 | Pubmed ID: 12032321 Discrimination Between Paralogs Using Microarray Analysis: Application to the Yap1p and Yap2p Transcriptional Networks Molecular Biology of the Cell. May, 2002 | Pubmed ID: 12006656 Nucleotides of Transcription Factor Binding Sites Exert Interdependent Effects on the Binding Affinities of Transcription Factors Nucleic Acids Research. Mar, 2002 | Pubmed ID: 11861919 Exploring the DNA-binding Specificities of Zinc Fingers with DNA Microarrays Proceedings of the National Academy of Sciences of the United States of America. Jun, 2001 | Pubmed ID: 11404456 Predicting Regulons and Their Cis-regulatory Motifs by Comparative Genomics Nucleic Acids Research. Nov, 2000 | Pubmed ID: 11071941 Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens Daniel J. Wiegand1,2, Henry H. Lee1, Nili Ostrov*1, George M. Church*1,2 1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering JoVE 59495 생체공학
Cell-free Protein Expression Using the Rapidly Growing Bacterium Vibrio natriegens Daniel J. Wiegand1,2, Henry H. Lee1, Nili Ostrov*1, George M. Church*1,2 1Department of Genetics, Harvard Medical School, 2Wyss Institute for Biologically Inspired Engineering JoVE 59495 생체공학