George Harauz Department of Molecular and Cellular Biology University of Guelph Biography Publications Institution JoVE Articles George Harauz has not added a biography. If you are George Harauz and would like to personalize this page please email our Author Liaison for assistance. Publications Classic 18.5- and 21.5-kDa Myelin Basic Protein Isoforms Associate with Cytoskeletal and SH3-Domain Proteins in the Immortalized N19-Oligodendroglial Cell Line Stimulated by Phorbol Ester and IGF-1 Neurochemical Research. Jan, 2012 | Pubmed ID: 22249765 Proline Substitutions and Threonine Pseudophosphorylation of the SH3 Ligand of 18.5-kDa Myelin Basic Protein Decrease Its Affinity for the Fyn-SH3 Domain and Alter Process Development and Protein Localization in Oligodendrocytes Journal of Neuroscience Research. Jan, 2012 | Pubmed ID: 21887699 Phosphorylation of Thellungiella Salsuginea Dehydrins TsDHN-1 and TsDHN-2 Facilitates Cation-induced Conformational Changes and Actin Assembly Biochemistry. Nov, 2011 | Pubmed ID: 21970344 Structured Functional Domains of Myelin Basic Protein: Cross Talk Between Actin Polymerization and Ca(2+)-dependent Calmodulin Interaction Biophysical Journal. Sep, 2011 | Pubmed ID: 21889463 Classical 18.5-and 21.5-kDa Isoforms of Myelin Basic Protein Inhibit Calcium Influx into Oligodendroglial Cells, in Contrast to Golli Isoforms Journal of Neuroscience Research. Apr, 2011 | Pubmed ID: 21312222 Classical 18.5-and 21.5-kDa Isoforms of Myelin Basic Protein Inhibit Calcium Influx into Oligodendroglial Cells, in Contrast to Golli Isoforms Journal of Neuroscience Research. Jan, 2011 | Pubmed ID: 21234892 Myelin Basic Protein Binds Microtubules to a Membrane Surface and to Actin Filaments in Vitro: Effect of Phosphorylation and Deimination Biochimica Et Biophysica Acta. Mar, 2011 | Pubmed ID: 21185260 Conformational Choreography of a Molecular Switch Region in Myelin Basic Protein--molecular Dynamics Shows Induced Folding and Secondary Structure Type Conversion Upon Threonyl Phosphorylation in Both Aqueous and Membrane-associated Environments Biochimica Et Biophysica Acta. Mar, 2011 | Pubmed ID: 21130728 Zinc Induces Disorder-to-order Transitions in Free and Membrane-associated Thellungiella Salsuginea Dehydrins TsDHN-1 and TsDHN-2: a Solution CD and Solid-state ATR-FTIR Study Amino Acids. May, 2011 | Pubmed ID: 20924623 Copper Uptake Induces Self-assembly of 18.5 KDa Myelin Basic Protein (MBP) Biophysical Journal. Nov, 2010 | Pubmed ID: 21044600 Interactions of Intrinsically Disordered Thellungiella Salsuginea Dehydrins TsDHN-1 and TsDHN-2 with Membranes - Synergistic Effects of Lipid Composition and Temperature on Secondary Structure Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. Oct, 2010 | Pubmed ID: 20921991 Secondary Structure and Solvent Accessibility of a Calmodulin-binding C-terminal Segment of Membrane-associated Myelin Basic Protein Biochemistry. Oct, 2010 | Pubmed ID: 20831157 Solid-state NMR Spectroscopy of Membrane-associated Myelin Basic Protein--conformation and Dynamics of an Immunodominant Epitope Biophysical Journal. Aug, 2010 | Pubmed ID: 20713009 Interaction of Myelin Basic Protein with Actin in the Presence of Dodecylphosphocholine Micelles Biochemistry. Aug, 2010 | Pubmed ID: 20593886 Fuzzy Complexes of Myelin Basic Protein: NMR Spectroscopic Investigations of a Polymorphic Organizational Linker of the Central Nervous System Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. Apr, 2010 | Pubmed ID: 20453917 The Interaction of Zinc with Membrane-associated 18.5 KDa Myelin Basic Protein: an Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopic Study Amino Acids. Aug, 2010 | Pubmed ID: 20169373 Misincorporation of the Proline Homologue Aze (azetidine-2-carboxylic Acid) into Recombinant Myelin Basic Protein Phytochemistry. Apr, 2010 | Pubmed ID: 20064647 Divalent Cations Induce a Compaction of Intrinsically Disordered Myelin Basic Protein Biochemical and Biophysical Research Communications. Jan, 2010 | Pubmed ID: 19903451 Structural Polymorphism and Multifunctionality of Myelin Basic Protein Biochemistry. Sep, 2009 | Pubmed ID: 19642704 The Classic Basic Protein of Myelin--conserved Structural Motifs and the Dynamic Molecular Barcode Involved in Membrane Adhesion and Protein-protein Interactions Current Protein & Peptide Science. Jun, 2009 | Pubmed ID: 19519451 Influence of Membrane Surface Charge and Post-translational Modifications to Myelin Basic Protein on Its Ability to Tether the Fyn-SH3 Domain to a Membrane in Vitro Biochemistry. Mar, 2009 | Pubmed ID: 19178193 Induced Secondary Structure and Polymorphism in an Intrinsically Disordered Structural Linker of the CNS: Solid-state NMR and FTIR Spectroscopy of Myelin Basic Protein Bound to Actin Biophysical Journal. Jan, 2009 | Pubmed ID: 19134474 Myelin Basic Protein Co-distributes with Other PI(4,5)P2-sequestering Proteins in Triton X-100 Detergent-resistant Membrane Microdomains Neuroscience Letters. Jan, 2009 | Pubmed ID: 19026719 Peptidylarginine Deiminase 2 (PAD2) Overexpression in Transgenic Mice Leads to Myelin Loss in the Central Nervous System Disease Models & Mechanisms. Nov-Dec, 2008 | Pubmed ID: 19093029 Kinetics of Human Peptidylarginine Deiminase 2 (hPAD2)--reduction of Ca2+ Dependence by Phospholipids and Assessment of Proposed Inhibition by Paclitaxel Side Chains Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. Oct, 2008 | Pubmed ID: 18923545 Myelin Basic Protein As a "PI(4,5)P2-modulin": a New Biological Function for a Major Central Nervous System Protein Biochemistry. Sep, 2008 | Pubmed ID: 18767817 Expression and Purification of the Active Variant of Recombinant Murine Golli-interacting Protein (GIP)--characterization of Its Phosphatase Activity and Interaction with Golli-BG21 Protein Expression and Purification. Nov, 2008 | Pubmed ID: 18625321 Solution NMR and CD Spectroscopy of an Intrinsically Disordered, Peripheral Membrane Protein: Evaluation of Aqueous and Membrane-mimetic Solvent Conditions for Studying the Conformational Adaptability of the 18.5 KDa Isoform of Myelin Basic Protein (MBP) European Biophysics Journal : EBJ. Jul, 2008 | Pubmed ID: 18449534 Backbone Dynamics of the 18.5 KDa Isoform of Myelin Basic Protein Reveals Transient Alpha-helices and a Calmodulin-binding Site Biophysical Journal. Jun, 2008 | Pubmed ID: 18326633 Dipolar Chemical Shift Correlation Spectroscopy for Homonuclear Carbon Distance Measurements in Proteins in the Solid State: Application to Structure Determination and Refinement Journal of the American Chemical Society. Jan, 2008 | Pubmed ID: 18072776 Binding of the Proline-rich Segment of Myelin Basic Protein to SH3 Domains: Spectroscopic, Microarray, and Modeling Studies of Ligand Conformation and Effects of Posttranslational Modifications Biochemistry. Jan, 2008 | Pubmed ID: 18067320 NMR Assignment of an Intrinsically Disordered Protein Under Physiological Conditions: the 18.5 KDa Isoform of Murine Myelin Basic Protein Biomolecular NMR Assignments. Jul, 2007 | Pubmed ID: 19636827 Solid-state NMR Spectroscopy of 18.5 KDa Myelin Basic Protein Reconstituted with Lipid Vesicles: Spectroscopic Characterisation and Spectral Assignments of Solvent-exposed Protein Fragments Biochimica Et Biophysica Acta. Dec, 2007 | Pubmed ID: 17920035 The BG21 Isoform of Golli Myelin Basic Protein is Intrinsically Disordered with a Highly Flexible Amino-terminal Domain Biochemistry. Aug, 2007 | Pubmed ID: 17676872 Molecular "negativity" May Underlie Multiple Sclerosis: Role of the Myelin Basic Protein Family in the Pathogenesis of MS International Review of Neurobiology. 2007 | Pubmed ID: 17531841 Purification and Spectroscopic Characterization of the Recombinant BG21 Isoform of Murine Golli Myelin Basic Protein Journal of Neuroscience Research. Feb, 2007 | Pubmed ID: 17131428 White Matter Rafting--membrane Microdomains in Myelin Neurochemical Research. Feb, 2007 | Pubmed ID: 17031566 A Tale of Two Citrullines--structural and Functional Aspects of Myelin Basic Protein Deimination in Health and Disease Neurochemical Research. Feb, 2007 | Pubmed ID: 16900293 Partitioning of Myelin Basic Protein into Membrane Microdomains in a Spontaneously Demyelinating Mouse Model for Multiple Sclerosis Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. Dec, 2006 | Pubmed ID: 17215885 Structural Studies of FlaA1 from Helicobacter Pylori Reveal the Mechanism for Inverting 4,6-dehydratase Activity The Journal of Biological Chemistry. Aug, 2006 | Pubmed ID: 16651261 Deimination of Membrane-bound Myelin Basic Protein in Multiple Sclerosis Exposes an Immunodominant Epitope Proceedings of the National Academy of Sciences of the United States of America. Mar, 2006 | Pubmed ID: 16537438 Solution NMR Structure of an Immunodominant Epitope of Myelin Basic Protein. Conformational Dependence on Environment of an Intrinsically Unstructured Protein The FEBS Journal. Feb, 2006 | Pubmed ID: 16420483 Assembly of Tubulin by Classic Myelin Basic Protein Isoforms and Regulation by Post-translational Modification Biochemistry. Dec, 2005 | Pubmed ID: 16342957 Regulation of Fish Gill Na(+)-K(+)-ATPase by Selective Sulfatide-enriched Raft Partitioning During Seawater Adaptation The Journal of Biological Chemistry. Nov, 2005 | Pubmed ID: 16096276 Effect of Arginine Loss in Myelin Basic Protein, As Occurs in Its Deiminated Charge Isoform, on Mediation of Actin Polymerization and Actin Binding to a Lipid Membrane in Vitro Biochemistry. Mar, 2005 | Pubmed ID: 15736962 Charge Effects Modulate Actin Assembly by Classic Myelin Basic Protein Isoforms Biochemical and Biophysical Research Communications. Apr, 2005 | Pubmed ID: 15721315 Electron Paramagnetic Resonance Spectroscopy and Molecular Modelling of the Interaction of Myelin Basic Protein (MBP) with Calmodulin (CaM)-diversity and Conformational Adaptability of MBP CaM-targets Journal of Structural Biology. Dec, 2004 | Pubmed ID: 15522783 Backbone Resonance Assignments of the 18.5 KDa Isoform of Murine Myelin Basic Protein (MBP) Journal of Biomolecular NMR. Aug, 2004 | Pubmed ID: 15243191 Myelin Basic Protein-diverse Conformational States of an Intrinsically Unstructured Protein and Its Roles in Myelin Assembly and Multiple Sclerosis Micron (Oxford, England : 1993). 2004 | Pubmed ID: 15219899 An Immunodominant Epitope of Myelin Basic Protein is an Amphipathic Alpha-helix The Journal of Biological Chemistry. Feb, 2004 | Pubmed ID: 14630913 Translocation of Group 1 Capsular Polysaccharide in Escherichia Coli Serotype K30. Structural and Functional Analysis of the Outer Membrane Lipoprotein Wza The Journal of Biological Chemistry. Dec, 2003 | Pubmed ID: 14522970 Myelin Basic Protein Has Multiple Calmodulin-binding Sites Biochemical and Biophysical Research Communications. Aug, 2003 | Pubmed ID: 12901870 Molecular Dynamics Exposes Alpha-helices in Myelin Basic Protein Journal of Molecular Modeling. Oct, 2003 | Pubmed ID: 12898292 Interaction of the 18.5-kD Isoform of Myelin Basic Protein with Ca2+ -calmodulin: Effects of Deimination Assessed by Intrinsic Trp Fluorescence Spectroscopy, Dynamic Light Scattering, and Circular Dichroism Protein Science : a Publication of the Protein Society. Jul, 2003 | Pubmed ID: 12824496 Membrane-anchoring and Charge Effects in the Interaction of Myelin Basic Protein with Lipid Bilayers Studied by Site-directed Spin Labeling The Journal of Biological Chemistry. Aug, 2003 | Pubmed ID: 12748174 Terminal Deletion Mutants of Myelin Basic Protein: New Insights into Self-association and Phospholipid Interactions Micron (Oxford, England : 1993). 2003 | Pubmed ID: 12694855 Expression and Properties of the Recombinant Murine Golli-myelin Basic Protein Isoform J37 Journal of Neuroscience Research. Mar, 2003 | Pubmed ID: 12605403 Interactions of the 18.5 KDa Isoform of Myelin Basic Protein with Ca2+-calmodulin: in Vitro Studies Using Gel Shift Assays Molecular and Cellular Biochemistry. Dec, 2002 | Pubmed ID: 12482024 Interactions of the 18.5-kDa Isoform of Myelin Basic Protein with Ca(2+)-calmodulin: in Vitro Studies Using Fluorescence Microscopy and Spectroscopy Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire. 2002 | Pubmed ID: 12234092 An Arg/Lys-->Gln Mutant of Recombinant Murine Myelin Basic Protein As a Mimic of the Deiminated Form Implicated in Multiple Sclerosis Protein Expression and Purification. Jul, 2002 | Pubmed ID: 12135568 The Formation of Helical Tubular Vesicles by Binary Monolayers Containing a Nickel-chelating Lipid and Phosphoinositides in the Presence of Basic Polypeptides Chemistry and Physics of Lipids. Jan, 2002 | Pubmed ID: 11841829 Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization Graham S.T. Smith1, Janine A.M. Voyer-Grant1, George Harauz1 1Department of Molecular and Cellular Biology, University of Guelph JoVE 3422 신경과학
Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization Graham S.T. Smith1, Janine A.M. Voyer-Grant1, George Harauz1 1Department of Molecular and Cellular Biology, University of Guelph JoVE 3422 신경과학