Jason Moffat Donnelly Centre University of Toronto Biography Publications Institution JoVE Articles Jason Moffat has not added a biography. If you are Jason Moffat and would like to personalize this page please email our Author Liaison for assistance. Publications CRISPR Screens Are Feasible in TP53 Wild-type Cells Molecular Systems Biology. Aug, 2019 | Pubmed ID: 31464370 Tailored Tetravalent Antibodies Potently and Specifically Activate Wnt/Frizzled Pathways in Cells, Organoids and Mice ELife. Aug, 2019 | Pubmed ID: 31452509 Identifying Chemogenetic Interactions from CRISPR Screens with DrugZ Genome Medicine. Aug, 2019 | Pubmed ID: 31439014 Bmi1 Regulates Human Glioblastoma Stem Cells Through Activation of Differential Gene Networks in CD133+ Brain Tumor Initiating Cells Journal of Neuro-oncology. Jul, 2019 | Pubmed ID: 31115870 Genetic Interaction Networks in Cancer Cells Current Opinion in Genetics & Development. Apr, 2019 | Pubmed ID: 30974317 Essential Gene Profiles for Human Pluripotent Stem Cells Identify Uncharacterized Genes and Substrate Dependencies Cell Reports. Apr, 2019 | Pubmed ID: 30970261 Global Genetic Networks and the Genotype-to-Phenotype Relationship Cell. Mar, 2019 | Pubmed ID: 30901552 Structure-guided Design Fine-tunes Pharmacokinetics, Tolerability, and Antitumor Profile of Multispecific Frizzled Antibodies Proceedings of the National Academy of Sciences of the United States of America. 04, 2019 | Pubmed ID: 30894493 Yeast Two-Hybrid Analysis for Ubiquitin Variant Inhibitors of Human Deubiquitinases Journal of Molecular Biology. Mar, 2019 | Pubmed ID: 30763569 Structural and Functional Characterization of Ubiquitin Variant Inhibitors of USP15 Structure (London, England : 1993). Apr, 2019 | Pubmed ID: 30713027 A Rapid in Vitro Methodology for Simultaneous Target Discovery and Antibody Generation Against Functional Cell Subpopulations Scientific Reports. Jan, 2019 | Pubmed ID: 30696911 Functional Genomic Characterization of a Synthetic Anti-HER3 Antibody Reveals a Role for Ubiquitination by RNF41 in the Anti-proliferative Response The Journal of Biological Chemistry. 01, 2019 | Pubmed ID: 30523157 Pooled Lentiviral CRISPR-Cas9 Screens for Functional Genomics in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30324523 EPH Profiling of BTIC Populations in Glioblastoma Multiforme Using CyTOF Methods in Molecular Biology (Clifton, N.J.). 2019 | Pubmed ID: 30324522 Protocadherin-1 is Essential for Cell Entry by New World Hantaviruses Nature. 11, 2018 | Pubmed ID: 30464266 Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons Molecular Cell. 11, 2018 | Pubmed ID: 30388412 A Synthetic Anti-Frizzled Antibody Engineered for Broadened Specificity Exhibits Enhanced Anti-tumor Properties MAbs. Nov-Dec, 2018 | Pubmed ID: 30183492 The Shieldin Complex Mediates 53BP1-dependent DNA Repair Nature. 08, 2018 | Pubmed ID: 30022168 CRISPR Screens Identify Genomic Ribonucleotides As a Source of PARP-trapping Lesions Nature. 07, 2018 | Pubmed ID: 29973717 Cotargeting Ephrin Receptor Tyrosine Kinases A2 and A3 in Cancer Stem Cells Reduces Growth of Recurrent Glioblastoma Cancer Research. Sep, 2018 | Pubmed ID: 29945963 Map of Synthetic Rescue Interactions for the Fanconi Anemia DNA Repair Pathway Identifies USP48 Nature Communications. 06, 2018 | Pubmed ID: 29891926 The Inhibitory NKR-P1B:Clr-b Recognition Axis Facilitates Detection of Oncogenic Transformation and Cancer Immunosurveillance Cancer Research. 07, 2018 | Pubmed ID: 29691253 Highly Multiplexed and Quantitative Cell-surface Protein Profiling Using Genetically Barcoded Antibodies Proceedings of the National Academy of Sciences of the United States of America. 03, 2018 | Pubmed ID: 29476010 Emerging and Evolving Concepts in Gene Essentiality Nature Reviews. Genetics. Jan, 2018 | Pubmed ID: 29033457 RNAi Screen Identifies Essential Regulators of Human Brain Metastasis-initiating Cells Acta Neuropathologica. 12, 2017 | Pubmed ID: 28766011 Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens G3 (Bethesda, Md.). 08, 2017 | Pubmed ID: 28655737 Generation and Validation of Intracellular Ubiquitin Variant Inhibitors for USP7 and USP10 Journal of Molecular Biology. Nov, 2017 | Pubmed ID: 28587923 SMYD2 Lysine Methyltransferase Regulates Leukemia Cell Growth and Regeneration After Genotoxic Stress Oncotarget. Mar, 2017 | Pubmed ID: 28187429 Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors Molecular Cell. Feb, 2017 | Pubmed ID: 28157508 Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells ACS Synthetic Biology. 04, 2017 | Pubmed ID: 28033709 Genome-wide CRISPR Screens Reveal a Wnt-FZD5 Signaling Circuit As a Druggable Vulnerability of RNF43-mutant Pancreatic Tumors Nature Medicine. Jan, 2017 | Pubmed ID: 27869803 Kin17 Facilitates Multiple Double-strand Break Repair Pathways That Govern B Cell Class Switching Scientific Reports. 11, 2016 | Pubmed ID: 27853268 Efficient Generation of Patient-matched Malignant and Normal Primary Cell Cultures from Clear Cell Renal Cell Carcinoma Patients: Clinically Relevant Models for Research and Personalized Medicine BMC Cancer. 07, 2016 | Pubmed ID: 27422173 The SAGA Deubiquitination Module Promotes DNA Repair and Class Switch Recombination Through ATM and DNAPK-Mediated γH2AX Formation Cell Reports. 05, 2016 | Pubmed ID: 27160905 BAGEL: a Computational Framework for Identifying Essential Genes from Pooled Library Screens BMC Bioinformatics. Apr, 2016 | Pubmed ID: 27083490 Inhibition of SCF Ubiquitin Ligases by Engineered Ubiquitin Variants That Target the Cul1 Binding Site on the Skp1-F-box Interface Proceedings of the National Academy of Sciences of the United States of America. Mar, 2016 | Pubmed ID: 26976582 System-Wide Modulation of HECT E3 Ligases with Selective Ubiquitin Variant Probes Molecular Cell. Apr, 2016 | Pubmed ID: 26949039 Pooled Screening for Antiproliferative Inhibitors of Protein-protein Interactions Nature Chemical Biology. Apr, 2016 | Pubmed ID: 26900867 Cytokinetic Effects of Wee1 Disruption in Pancreatic Cancer Cell Cycle (Georgetown, Tex.). 2016 | Pubmed ID: 26890070 Automated Inference Procedure for the Determination of Cell Growth Parameters Physical Review. E. Jan, 2016 | Pubmed ID: 26871096 Functional Genomic Landscape of Human Breast Cancer Drivers, Vulnerabilities, and Resistance Cell. Jan, 2016 | Pubmed ID: 26771497 SMN and Symmetric Arginine Dimethylation of RNA Polymerase II C-terminal Domain Control Termination Nature. Jan, 2016 | Pubmed ID: 26700805 Identification of RNF168 As a PML Nuclear Body Regulator Journal of Cell Science. Feb, 2016 | Pubmed ID: 26675234 Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy Between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells Cell Reports. Dec, 2015 | Pubmed ID: 26673326 A Dynamic Protein Interaction Landscape of the Human Centrosome-Cilium Interface Cell. Dec, 2015 | Pubmed ID: 26638075 High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities Cell. Dec, 2015 | Pubmed ID: 26627737 Dependence of Human Colorectal Cells Lacking the FBW7 Tumor Suppressor on the Spindle Assembly Checkpoint Genetics. Nov, 2015 | Pubmed ID: 26354767 Genome-wide RNAi Analysis Reveals That Simultaneous Inhibition of Specific Mevalonate Pathway Genes Potentiates Tumor Cell Death Oncotarget. Sep, 2015 | Pubmed ID: 26353928 Selection of Recombinant Anti-SH3 Domain Antibodies by High-throughput Phage Display Protein Science : a Publication of the Protein Society. Nov, 2015 | Pubmed ID: 26332758 Identification of P450 Oxidoreductase As a Major Determinant of Sensitivity to Hypoxia-Activated Prodrugs Cancer Research. Oct, 2015 | Pubmed ID: 26297733 A High Through-put Platform for Recombinant Antibodies to Folded Proteins Molecular & Cellular Proteomics : MCP. Oct, 2015 | Pubmed ID: 26290498 Extensive Mapping of an Innate Immune Network with CRISPR Molecular Systems Biology. Jul, 2015 | Pubmed ID: 26208794 Natural Variation in Gene Expression Modulates the Severity of Mutant Phenotypes Cell. Jul, 2015 | Pubmed ID: 26186192 INNATE IMMUNITY. Cytosolic Detection of the Bacterial Metabolite HBP Activates TIFA-dependent Innate Immunity Science (New York, N.Y.). Jun, 2015 | Pubmed ID: 26068852 Inhibition of the Mitochondrial Protease ClpP As a Therapeutic Strategy for Human Acute Myeloid Leukemia Cancer Cell. Jun, 2015 | Pubmed ID: 26058080 CYCLoPs: A Comprehensive Database Constructed from Automated Analysis of Protein Abundance and Subcellular Localization Patterns in Saccharomyces Cerevisiae G3 (Bethesda, Md.). Apr, 2015 | Pubmed ID: 26048563 Yeast Proteome Dynamics from Single Cell Imaging and Automated Analysis Cell. Jun, 2015 | Pubmed ID: 26046442 RNA Interference Screen to Identify Kinases That Suppress Rescue of ΔF508-CFTR Molecular & Cellular Proteomics : MCP. Jun, 2015 | Pubmed ID: 25825526 Coiled-coil Domain Containing 68 (CCDC68) Demonstrates a Tumor-suppressive Role in Pancreatic Ductal Adenocarcinoma Oncogene. Aug, 2015 | Pubmed ID: 25381825 Differential Regulation of FGFR3 by PTPN1 and PTPN2 Proteomics. Jan, 2015 | Pubmed ID: 25311528 A Systematic Approach to Identify Novel Cancer Drug Targets Using Machine Learning, Inhibitor Design and High-throughput Screening Genome Medicine. 2014 | Pubmed ID: 25165489 Measuring Error Rates in Genomic Perturbation Screens: Gold Standards for Human Functional Genomics Molecular Systems Biology. Jul, 2014 | Pubmed ID: 24987113 A Genome Scale Overexpression Screen to Reveal Drug Activity in Human Cells Genome Medicine. 2014 | Pubmed ID: 24944581 The Mammalian-membrane Two-hybrid Assay (MaMTH) for Probing Membrane-protein Interactions in Human Cells Nature Methods. May, 2014 | Pubmed ID: 24658140 CD133-targeted Niche-dependent Therapy in Cancer: a Multipronged Approach The American Journal of Pathology. May, 2014 | Pubmed ID: 24589338 Post-translational Regulation of CD133 by ATase1/ATase2-mediated Lysine Acetylation Journal of Molecular Biology. May, 2014 | Pubmed ID: 24556617 Large-scale Interaction Profiling of PDZ Domains Through Proteomic Peptide-phage Display Using Human and Viral Phage Peptidomes Proceedings of the National Academy of Sciences of the United States of America. Feb, 2014 | Pubmed ID: 24550280 The RhoGEF GEF-H1 is Required for Oncogenic RAS Signaling Via KSR-1 Cancer Cell. Feb, 2014 | Pubmed ID: 24525234 ShRNA Kinome Screen Identifies TBK1 As a Therapeutic Target for HER2+ Breast Cancer Cancer Research. Apr, 2014 | Pubmed ID: 24487029 A Negative Genetic Interaction Map in Isogenic Cancer Cell Lines Reveals Cancer Cell Vulnerabilities Molecular Systems Biology. Oct, 2013 | Pubmed ID: 24104479 Targeting HER2+ Breast Cancer Cells: Lysosomal Accumulation of Anti-HER2 Antibodies is Influenced by Antibody Binding Site and Conjugation to Polymeric Nanoparticles Journal of Controlled Release : Official Journal of the Controlled Release Society. Dec, 2013 | Pubmed ID: 23880472 Integrated Genomic, Transcriptomic, and RNA-interference Analysis of Genes in Somatic Copy Number Gains in Pancreatic Ductal Adenocarcinoma Pancreas. Aug, 2013 | Pubmed ID: 23851435 Miniature Short Hairpin RNA Screens to Characterize Antiproliferative Drugs G3 (Bethesda, Md.). Aug, 2013 | Pubmed ID: 23797109 RNA Interference Screens to Uncover Membrane Protein Biology Briefings in Functional Genomics. Sep, 2013 | Pubmed ID: 23793263 MBNL Proteins Repress ES-cell-specific Alternative Splicing and Reprogramming Nature. Jun, 2013 | Pubmed ID: 23739326 A Strategy for Modulation of Enzymes in the Ubiquitin System Science (New York, N.Y.). Feb, 2013 | Pubmed ID: 23287719 Regulation of CD133 by HDAC6 Promotes β-catenin Signaling to Suppress Cancer Cell Differentiation Cell Reports. Oct, 2012 | Pubmed ID: 23084749 Suppression of Cancer Progression by MGAT1 ShRNA Knockdown PloS One. 2012 | Pubmed ID: 22957033 Dissecting DNA Damage Response Pathways by Analysing Protein Localization and Abundance Changes During DNA Replication Stress Nature Cell Biology. Sep, 2012 | Pubmed ID: 22842922 Developmentally Programmed Nuclear Destruction During Yeast Gametogenesis Developmental Cell. Jul, 2012 | Pubmed ID: 22727375 A Versatile Lentiviral Expression System to Identify Mammalian Protein-protein Interactions Methods (San Diego, Calif.). Aug, 2012 | Pubmed ID: 22713554 Essential Gene Profiles in Breast, Pancreatic, and Ovarian Cancer Cells Cancer Discovery. Feb, 2012 | Pubmed ID: 22585861 The Mixed Lineage Leukemia (MLL) Fusion-associated Gene AF4 Promotes CD133 Transcription Cancer Research. Apr, 2012 | Pubmed ID: 22337994 COLT-Cancer: Functional Genetic Screening Resource for Essential Genes in Human Cancer Cell Lines Nucleic Acids Research. Jan, 2012 | Pubmed ID: 22102578 Control of the RNA Polymerase II Phosphorylation State in Promoter Regions by CTD Interaction Domain-containing Proteins RPRD1A and RPRD1B Transcription. Sep-Oct, 2011 | Pubmed ID: 22231121 Inhibition of Mitochondrial Translation As a Therapeutic Strategy for Human Acute Myeloid Leukemia Cancer Cell. Nov, 2011 | Pubmed ID: 22094260 CD133 Protein N-glycosylation Processing Contributes to Cell Surface Recognition of the Primitive Cell Marker AC133 Epitope The Journal of Biological Chemistry. Nov, 2011 | Pubmed ID: 21937449 SnapShot: High-throughput Sequencing Applications Cell. Sep, 2011 | Pubmed ID: 21925324 Pooled Lentiviral ShRNA Screening for Functional Genomics in Mammalian Cells Methods in Molecular Biology (Clifton, N.J.). 2011 | Pubmed ID: 21877282 A Comprehensive Platform for Highly Multiplexed Mammalian Functional Genetic Screens BMC Genomics. 2011 | Pubmed ID: 21548937 SATB2 Augments ΔNp63α in Head and Neck Squamous Cell Carcinoma EMBO Reports. Oct, 2010 | Pubmed ID: 20829881 Selective Targeting of Neuroblastoma Tumour-initiating Cells by Compounds Identified in Stem Cell-based Small Molecule Screens EMBO Molecular Medicine. Sep, 2010 | Pubmed ID: 20721990 A Lentiviral Functional Proteomics Approach Identifies Chromatin Remodeling Complexes Important for the Induction of Pluripotency Molecular & Cellular Proteomics : MCP. May, 2010 | Pubmed ID: 20305087 The Ubiquitin-activating Enzyme E1 As a Therapeutic Target for the Treatment of Leukemia and Multiple Myeloma Blood. Mar, 2010 | Pubmed ID: 20075161 Dual Regulation by Pairs of Cyclin-dependent Protein Kinases and Histone Deacetylases Controls G1 Transcription in Budding Yeast PLoS Biology. Sep, 2009 | Pubmed ID: 19823668 A Role for the TGFbeta-Par6 Polarity Pathway in Breast Cancer Progression Proceedings of the National Academy of Sciences of the United States of America. Aug, 2009 | Pubmed ID: 19667198 Exploration of Essential Gene Functions Via Titratable Promoter Alleles Cell. Jul, 2004 | Pubmed ID: 15242642 Late-G1 Cyclin-CDK Activity is Essential for Control of Cell Morphogenesis in Budding Yeast Nature Cell Biology. Jan, 2004 | Pubmed ID: 14688790 Ac'septin' a Signal: Kinase Regulation by Septins Developmental Cell. Oct, 2003 | Pubmed ID: 14536051 Dissection of a Complex Phenotype by Functional Genomics Reveals Roles for the Yeast Cyclin-dependent Protein Kinase Pho85 in Stress Adaptation and Cell Integrity Molecular and Cellular Biology. Jul, 2002 | Pubmed ID: 12077337 Transcriptional Coregulation by the Cell Integrity Mitogen-activated Protein Kinase Slt2 and the Cell Cycle Regulator Swi4 Molecular and Cellular Biology. Oct, 2001 | Pubmed ID: 11533240 Mammalian Cdk5 is a Functional Homologue of the Budding Yeast Pho85 Cyclin-dependent Protein Kinase Proceedings of the National Academy of Sciences of the United States of America. Dec, 1999 | Pubmed ID: 10588725 Cyclin Partners Determine Pho85 Protein Kinase Substrate Specificity in Vitro and in Vivo: Control of Glycogen Biosynthesis by Pcl8 and Pcl10 Molecular and Cellular Biology. Jun, 1998 | Pubmed ID: 9584169 Gepoolde CRISPR-gebaseerde genetische schermen in zoogdiercellen Katherine Chan*1, Amy Hin Yan Tong*1, Kevin R Brown1, Patricia Mero1, Jason Moffat1,2,3 1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto JoVE 59780 유전학
Gepoolde CRISPR-gebaseerde genetische schermen in zoogdiercellen Katherine Chan*1, Amy Hin Yan Tong*1, Kevin R Brown1, Patricia Mero1, Jason Moffat1,2,3 1Donnelly Centre, University of Toronto, 2Department of Molecular Genetics, University of Toronto, 3Institute for Biomaterials and Biomedical Engineering, University of Toronto JoVE 59780 유전학