Nina Harmening Experimental Ophthalmology University of Geneva Biography Publications Institution JoVE Articles Nina Harmening Dr. rer. nat. Nina Harmening, PhD in biology, joined Prof. Thumann’s research group in 2009. She has extensive experience in the isolation and culture of primary cells and cell lines, non-viral gene delivery methodologies including electroporation, the use of the Sleeping Beauty transposon system, and molecular and biochemical analyses. She has been trained in GMP-compliant production of GTMPs (Gene Therapy Medicinal Product) for use in clinical trials, qualified to work under GMP-grade conditions and is the responsible person for production. Currently, she is working on the improvement of the safety profile of SB100X transfected cells. Publications Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by PFAR4 Plasmids Molecular Therapy. Nucleic Acids. Mar, 2017 | Pubmed ID: 28325297 The Antibiotic-free PFAR4 Vector Paired with the Sleeping Beauty Transposon System Mediates Efficient Transgene Delivery in Human Cells Molecular Therapy. Nucleic Acids. Month, | Pubmed ID: 29858090 Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 생물학 Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 의학 Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells Thais Bascuas1,2, Martina Kropp1,2, Nina Harmening1,2, Mohammed Asrih1, Zsuzsanna Izsvák3, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine JoVE 61957 의학
Isolation, Culture, and Genetic Engineering of Mammalian Primary Pigment Epithelial Cells for Non-Viral Gene Therapy Thais Bascuas*1,2, Martina Kropp*1,2, Nina Harmening1,2, Brittany M. Wong1, Sandra Johnen3, Zsuzsanna Izsvák4, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Department of Ophthalmology, University Hospital RWTH Aachen, 4Max Delbrück Center for Molecular Medicine JoVE 62145 생물학
Electroporation-Based Genetic Modification of Primary Human Pigment Epithelial Cells Using the Sleeping Beauty Transposon System Sandra Johnen1, Nina Harmening2,3, Corinne Marie4,5, Daniel Scherman4, Zsuzsanna Izsvák6, Zoltán Ivics7, Peter Walter1, Gabriele Thumann2,3 1Department of Ophthalmology, University Hospital RWTH Aachen, 2Experimental Ophthalmology, University of Geneva, 3Department of Ophthalmology, University Hospitals of Geneva, 4Université de Paris, CNRS, INSERM, UTCBS, Unité des technologies Chimiques et Biologiques pour la Santé, 5Chimie ParisTech, PSL Research University, 6Max Delbrück Center for Molecular Medicine in the Helmholtz Association, 7Division of Medical Biotechnology, Paul-Ehrlich-Institute JoVE 61987 의학
Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells Thais Bascuas1,2, Martina Kropp1,2, Nina Harmening1,2, Mohammed Asrih1, Zsuzsanna Izsvák3, Gabriele Thumann1,2 1Experimental Ophthalmology, University of Geneva, 2Department of Ophthalmology, University Hospitals of Geneva, 3Max Delbrück Center for Molecular Medicine JoVE 61957 의학