Paul M. W. French Photonics Group, Department of Physics Imperial College London Biography Publications Institution JoVE Articles Paul M. W. French has not added a biography. If you are Paul M. W. French and would like to personalize this page please email our Author Liaison for assistance. Publications Conformational Transition of FGFR Kinase Activation Revealed by Site-specific Unnatural Amino Acid Reporter and Single Molecule FRET Scientific Reports. Jan, 2017 | Pubmed ID: 28045057 Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy ACS Nano. Nov, 2016 | Pubmed ID: 27794591 Adaptive Multiphoton Endomicroscopy Through a Dynamically Deformed Multicore Optical Fiber Using Proximal Detection Optics Express. Sep, 2016 | Pubmed ID: 27661887 Corrigendum: Screening for Protein-protein Interactions Using Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) Scientific Reports. Sep, 2016 | Pubmed ID: 27654516 EasySTORM: a Robust, Lower-cost Approach to Localisation and TIRF Microscopy Journal of Biophotonics. Sep, 2016 | Pubmed ID: 27592533 Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM Sensors (Basel, Switzerland). Aug, 2016 | Pubmed ID: 27548185 Screening for Protein-protein Interactions Using Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) Scientific Reports. Jun, 2016 | Pubmed ID: 27339025 Quantitative in Vivo Optical Tomography of Cancer Progression & Vasculature Development in Adult Zebrafish Oncotarget. Jul, 2016 | Pubmed ID: 27259259 Tunable Fibre-coupled Multiphoton Microscopy with a Negative Curvature Fibre Journal of Biophotonics. Jul, 2016 | Pubmed ID: 26989868 Visualising Apoptosis in Live Zebrafish Using Fluorescence Lifetime Imaging with Optical Projection Tomography to Map FRET Biosensor Activity in Space and Time Journal of Biophotonics. Apr, 2016 | Pubmed ID: 26753623 Time-resolved FRET Reports FGFR1 Dimerization and Formation of a Complex with Its Effector PLCγ1 Advances in Biological Regulation. Jan, 2016 | Pubmed ID: 26482290 Accelerated Optical Projection Tomography Applied to In Vivo Imaging of Zebrafish PloS One. 2015 | Pubmed ID: 26308086 Homo-FRET Based Biosensors and Their Application to Multiplexed Imaging of Signalling Events in Live Cells International Journal of Molecular Sciences. Jun, 2015 | Pubmed ID: 26133241 Correction Approach for Delta Function Convolution Model Fitting of Fluorescence Decay Data in the Case of a Monoexponential Reference Fluorophore Journal of Fluorescence. Sep, 2015 | Pubmed ID: 26063535 Mesoscopic in Vivo 3-D Tracking of Sparse Cell Populations Using Angular Multiplexed Optical Projection Tomography Biomedical Optics Express. Apr, 2015 | Pubmed ID: 25909009 Application of Time-resolved Autofluorescence to Label-free in Vivo Optical Mapping of Changes in Tissue Matrix and Metabolism Associated with Myocardial Infarction and Heart Failure Biomedical Optics Express. Feb, 2015 | Pubmed ID: 25780727 Remote Focal Scanning Optical Projection Tomography with an Electrically Tunable Lens Biomedical Optics Express. Oct, 2014 | Pubmed ID: 25360356 Analysis of DNA Binding and Nucleotide Flipping Kinetics Using Two-color Two-photon Fluorescence Lifetime Imaging Microscopy Analytical Chemistry. Nov, 2014 | Pubmed ID: 25303623 Introduction: Feature Issue on Optical Molecular Probes, Imaging, and Drug Delivery Biomedical Optics Express. Feb, 2014 | Pubmed ID: 24575356 Fluorescence Lifetime Spectroscopy of Tissue Autofluorescence in Normal and Diseased Colon Measured Ex Vivo Using a Fiber-optic Probe Biomedical Optics Express. Feb, 2014 | Pubmed ID: 24575345 3-D Stimulated Emission Depletion Microscopy with Programmable Aberration Correction Journal of Biophotonics. Jan, 2014 | Pubmed ID: 23788459 Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets PloS One. 2013 | Pubmed ID: 23940626 Multiphoton Laser Tomography and Fluorescence Lifetime Imaging of Melanoma: Morphologic Features and Quantitative Data for Sensitive and Specific Non-invasive Diagnostics PloS One. 2013 | Pubmed ID: 23923016 Simultaneous Angular Multiplexing Optical Projection Tomography at Shifted Focal Planes Optics Letters. Mar, 2013 | Pubmed ID: 23503237 Automated Fluorescence Lifetime Imaging Plate Reader and Its Application to Förster Resonant Energy Transfer Readout of Gag Protein Aggregation Journal of Biophotonics. May, 2013 | Pubmed ID: 23184449 Fluorescence Lifetime Readouts of Troponin-C-based Calcium FRET Sensors: a Quantitative Comparison of CFP and MTFP1 As Donor Fluorophores PloS One. 2012 | Pubmed ID: 23152874 Activity of PLCε Contributes to Chemotaxis of Fibroblasts Towards PDGF Journal of Cell Science. Dec, 2012 | Pubmed ID: 22992460 Multiphoton Multispectral Fluorescence Lifetime Tomography for the Evaluation of Basal Cell Carcinomas PloS One. 2012 | Pubmed ID: 22984428 Incorporation of an Experimentally Determined MTF for Spatial Frequency Filtering and Deconvolution During Optical Projection Tomography Reconstruction Optics Express. Mar, 2012 | Pubmed ID: 22453413 In Vivo Measurements of Diffuse Reflectance and Time-resolved Autofluorescence Emission Spectra of Basal Cell Carcinomas Journal of Biophotonics. Mar, 2012 | Pubmed ID: 22308093 Multiphoton Laser Microscopy and Fluorescence Lifetime Imaging for the Evaluation of the Skin Dermatology Research and Practice. 2012 | Pubmed ID: 22203841 Förster Resonance Energy Transfer Imaging in Vivo with Approximated Radiative Transfer Equation Applied Optics. Dec, 2011 | Pubmed ID: 22193187 Quantification of Cellular Autofluorescence of Human Skin Using Multiphoton Tomography and Fluorescence Lifetime Imaging in Two Spectral Detection Channels Biomedical Optics Express. Dec, 2011 | Pubmed ID: 22162820 Application of Ultrafast Gold Luminescence to Measuring the Instrument Response Function for Multispectral Multiphoton Fluorescence Lifetime Imaging Optics Express. Jul, 2011 | Pubmed ID: 21934746 Remodelling of Cortical Actin Where Lytic Granules Dock at Natural Killer Cell Immune Synapses Revealed by Super-resolution Microscopy PLoS Biology. Sep, 2011 | Pubmed ID: 21931537 In Vivo Fluorescence Lifetime Tomography of a FRET Probe Expressed in Mouse Biomedical Optics Express. Jul, 2011 | Pubmed ID: 21750768 In Vivo Fluorescence Lifetime Optical Projection Tomography Biomedical Optics Express. Apr, 2011 | Pubmed ID: 21559145 Adaptive Phase Compensation for Ultracompact Laser Scanning Endomicroscopy Optics Letters. May, 2011 | Pubmed ID: 21540976 FLIM FRET Technology for Drug Discovery: Automated Multiwell-plate High-content Analysis, Multiplexed Readouts and Application in Situ Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry. Feb, 2011 | Pubmed ID: 21337485 Membrane Environment Exerts an Important Influence on Rac-mediated Activation of Phospholipase Cγ2 Molecular and Cellular Biology. Mar, 2011 | Pubmed ID: 21245382 Differential Modes of DNA Binding by Mismatch Uracil DNA Glycosylase from Escherichia Coli: Implications for Abasic Lesion Processing and Enzyme Communication in the Base Excision Repair Pathway Nucleic Acids Research. Apr, 2011 | Pubmed ID: 21112870 Wide-field Fluorescence Lifetime Imaging of Cancer Biomedical Optics Express. Aug, 2010 | Pubmed ID: 21258496 High Plasma Membrane Lipid Order Imaged at the Immunological Synapse Periphery in Live T Cells Molecular Membrane Biology. Aug, 2010 | Pubmed ID: 20540668 Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse Science Signaling. 2010 | Pubmed ID: 20460647 A Fluorescence Lifetime Imaging Scanning Confocal Endomicroscope Journal of Biophotonics. Jan, 2010 | Pubmed ID: 19787682 Three-dimensional Imaging of Förster Resonance Energy Transfer in Heterogeneous Turbid Media by Tomographic Fluorescent Lifetime Imaging Optics Letters. Sep, 2009 | Pubmed ID: 19756100 Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling Within the Docking Structure of Natural Killer Cell Immune Synapses Biophysical Journal. Jan, 2009 | Pubmed ID: 19167281 Continuous-flow Polymerase Chain Reaction of Single-copy DNA in Microfluidic Microdroplets Analytical Chemistry. Jan, 2009 | Pubmed ID: 19055421 High Speed Unsupervised Fluorescence Lifetime Imaging Confocal Multiwell Plate Reader for High Content Analysis Journal of Biophotonics. Dec, 2008 | Pubmed ID: 19343677 A Compact, Multidimensional Spectrofluorometer Exploiting Supercontinuum Generation Journal of Biophotonics. Dec, 2008 | Pubmed ID: 19343675 Fluorescence Lifetime Optical Projection Tomography Journal of Biophotonics. Oct, 2008 | Pubmed ID: 19343662 Multiplexed FRET to Image Multiple Signaling Events in Live Cells Biophysical Journal. Nov, 2008 | Pubmed ID: 18757561 High-speed High-resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers Biophysical Journal. Nov, 2008 | Pubmed ID: 18723590 Three-dimensional Molecular Mapping in a Microfluidic Mixing Device Using Fluorescence Lifetime Imaging Optics Letters. Aug, 2008 | Pubmed ID: 18709122 Improved Sectioning in a Slit Scanning Confocal Microscope Optics Letters. Aug, 2008 | Pubmed ID: 18709096 Stimulated Emission Depletion Microscopy with a Supercontinuum Source and Fluorescence Lifetime Imaging Optics Letters. Jan, 2008 | Pubmed ID: 18197209 Excitation-resolved Hyperspectral Fluorescence Lifetime Imaging Using a UV-extended Supercontinuum Source Optics Letters. Dec, 2007 | Pubmed ID: 18059949 Fluorescence Lifetime Imaging by Using Time-gated Data Acquisition Applied Optics. Oct, 2007 | Pubmed ID: 17952172 Optical Techniques for Imaging Membrane Lipid Microdomains in Living Cells Seminars in Cell & Developmental Biology. Oct, 2007 | Pubmed ID: 17728161 Fluorescence-lifetime Imaging of DNA-dye Interactions Within Continuous-flow Microfluidic Systems Angewandte Chemie (International Ed. in English). 2007 | Pubmed ID: 17436333 Rapid Hyperspectral Fluorescence Lifetime Imaging Microscopy Research and Technique. May, 2007 | Pubmed ID: 17366615 Tissue Characterization Using Dimensionality Reduction and Fluorescence Imaging Medical Image Computing and Computer-assisted Intervention : MICCAI ... International Conference on Medical Image Computing and Computer-Assisted Intervention. 2006 | Pubmed ID: 17354820 Structurally Distinct Membrane Nanotubes Between Human Macrophages Support Long-distance Vesicular Traffic or Surfing of Bacteria Journal of Immunology (Baltimore, Md. : 1950). Dec, 2006 | Pubmed ID: 17142745 Microclusters of Inhibitory Killer Immunoglobulin-like Receptor Signaling at Natural Killer Cell Immunological Synapses The Journal of Cell Biology. Jul, 2006 | Pubmed ID: 16801390 Fluorescence Lifetime Imaging Provides Enhanced Contrast when Imaging the Phase-sensitive Dye Di-4-ANEPPDHQ in Model Membranes and Live Cells Biophysical Journal. Jun, 2006 | Pubmed ID: 16617080 Quantitative 3D Mapping of Fluidic Temperatures Within Microchannel Networks Using Fluorescence Lifetime Imaging Analytical Chemistry. Apr, 2006 | Pubmed ID: 16579608 Time-resolved Fluorescence Microscopy Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology. Jan, 2005 | Pubmed ID: 15616687 Fluorescence Imaging of Two-photon Linear Dichroism: Cholesterol Depletion Disrupts Molecular Orientation in Cell Membranes Biophysical Journal. Jan, 2005 | Pubmed ID: 15520272 Holographic Optical Coherence Imaging of Rat Osteogenic Sarcoma Tumor Spheroids Applied Optics. Sep, 2004 | Pubmed ID: 15449473 Time-resolved Fluorescence Anisotropy Imaging Applied to Live Cells Optics Letters. Mar, 2004 | Pubmed ID: 15035478 Studying Biological Tissue with Fluorescence Lifetime Imaging: Microscopy, Endoscopy, and Complex Decay Profiles Applied Optics. Jun, 2003 | Pubmed ID: 12790450 Fluorescence Lifetime Imaging of Unstained Tissues: Early Results in Human Breast Cancer The Journal of Pathology. Mar, 2003 | Pubmed ID: 12579532 Imaging the Environment of Green Fluorescent Protein Biophysical Journal. Dec, 2002 | Pubmed ID: 12496126 Imaging Immune Surveillance by T Cells and NK Cells Immunological Reviews. Nov, 2002 | Pubmed ID: 12445274 Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy Frederik Görlitz*1, Douglas J. Kelly*1, Sean C. Warren1, Dominic Alibhai2, Lucien West3, Sunil Kumar1, Yuriy Alexandrov1, Ian Munro1, Edwin Garcia1, James McGinty1, Clifford Talbot1, Remigiusz A. Serwa4, Emmanuelle Thinon4, Vincenzo da Paola3, Edward J. Murray5, Frank Stuhmeier6, Mark A. A. Neil1, Edward W. Tate4, Christopher Dunsby1,7, Paul M. W. French1 1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London JoVE 55119 생물학
Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy Frederik Görlitz*1, Douglas J. Kelly*1, Sean C. Warren1, Dominic Alibhai2, Lucien West3, Sunil Kumar1, Yuriy Alexandrov1, Ian Munro1, Edwin Garcia1, James McGinty1, Clifford Talbot1, Remigiusz A. Serwa4, Emmanuelle Thinon4, Vincenzo da Paola3, Edward J. Murray5, Frank Stuhmeier6, Mark A. A. Neil1, Edward W. Tate4, Christopher Dunsby1,7, Paul M. W. French1 1Photonics Group, Department of Physics, Imperial College London, 2Institute for Chemical Biology, Department of Chemistry, Imperial College London, 3MRC Clinical Sciences Centre, Hammersmith Hospital, 4Chemical Biology Section, Department of Chemistry, Imperial College London, 5Retroscreen Virology Ltd, 6Pfizer Global Research and Development, Pfizer Limited, Sandwich, Kent, UK, 7Centre for Histopathology, Imperial College London JoVE 55119 생물학