Walter Keller Computational and Systems Biology, Biozentrum University of Basel Biography Publications Institution JoVE Articles Walter Keller has not added a biography. If you are Walter Keller and would like to personalize this page please email our Author Liaison for assistance. Publications Clustering of Conformational IgE Epitopes on the Major Dog Allergen Can F 1 Scientific Reports. Sep, 2017 | Pubmed ID: 28939849 Targeting Type IV Secretion System Proteins to Combat Multidrug-Resistant Gram-positive Pathogens The Journal of Infectious Diseases. Jun, 2017 | Pubmed ID: 28863473 The Coordinated Action of Calcineurin and Cathepsin D Protects Against α-Synuclein Toxicity Frontiers in Molecular Neuroscience. 2017 | Pubmed ID: 28713240 Conjugative Type IV Secretion in Gram-positive Pathogens: TraG, a Lytic Transglycosylase and Endopeptidase, Interacts with Translocation Channel Protein TraM Plasmid. May, 2017 | Pubmed ID: 28219792 Mitochondrial Lipids in Neurodegeneration Cell and Tissue Research. Jan, 2017 | Pubmed ID: 27449929 Critical and Direct Involvement of the CD23 Stalk Region in IgE Binding The Journal of Allergy and Clinical Immunology. Jan, 2017 | Pubmed ID: 27343203 Molecular, Structural and Immunological Characterization of Der P 18, a Chitinase-Like House Dust Mite Allergen PloS One. 2016 | Pubmed ID: 27548813 A Comprehensive Analysis of 3' End Sequencing Data Sets Reveals Novel Polyadenylation Signals and the Repressive Role of Heterogeneous Ribonucleoprotein C on Cleavage and Polyadenylation Genome Research. 08, 2016 | Pubmed ID: 27382025 VirB8-like Protein TraH is Crucial for DNA Transfer in Enterococcus Faecalis Scientific Reports. 04, 2016 | Pubmed ID: 27103580 IgE Epitope Proximity Determines Immune Complex Shape and Effector Cell Activation Capacity The Journal of Allergy and Clinical Immunology. May, 2016 | Pubmed ID: 26684291 1H, 15N and 13C Chemical Shift Assignment of the Gram-positive Conjugative Transfer Protein TraHpIP501 Biomolecular NMR Assignments. Apr, 2016 | Pubmed ID: 26559076 Tracking Morphologies at the Nanoscale: Self-assembly of an Amphiphilic Designer Peptide into a Double Helix Superstructure Nano Research. Jun, 2015 | Pubmed ID: 26290684 Cor a 14, the Allergenic 2S Albumin from Hazelnut, is Highly Thermostable and Resistant to Gastrointestinal Digestion Molecular Nutrition & Food Research. Oct, 2015 | Pubmed ID: 26178695 Reflections on the RNA World RNA (New York, N.Y.). Apr, 2015 | Pubmed ID: 25780126 Comparison of the Specificities of IgG, IgG-subclass, IgA and IgM Reactivities in African and European HIV-infected Individuals with an HIV-1 Clade C Proteome-based Array PloS One. 2015 | Pubmed ID: 25658330 Conjugation in Gram-Positive Bacteria Microbiology Spectrum. Aug, 2014 | Pubmed ID: 26104193 Global 3' UTR Shortening Has a Limited Effect on Protein Abundance in Proliferating T Cells Nature Communications. 2014 | Pubmed ID: 25413384 Biochemical, Biophysical and IgE-epitope Characterization of the Wheat Food Allergen, Tri a 37 PloS One. 2014 | Pubmed ID: 25368998 Reconstitution of CPSF Active in Polyadenylation: Recognition of the Polyadenylation Signal by WDR33 Genes & Development. Nov, 2014 | Pubmed ID: 25301781 Structure of the Double-stranded DNA-binding Type IV Secretion Protein TraN from Enterococcus Acta Crystallographica. Section D, Biological Crystallography. Sep, 2014 | Pubmed ID: 25195751 The Type IV Secretion Protein TraK from the Enterococcus Conjugative Plasmid PIP501 Exhibits a Novel Fold Acta Crystallographica. Section D, Biological Crystallography. Apr, 2014 | Pubmed ID: 24699656 Means to an End: Mechanisms of Alternative Polyadenylation of Messenger RNA Precursors Wiley Interdisciplinary Reviews. RNA. Mar-Apr, 2014 | Pubmed ID: 24243805 Dissection of the IgE and T-cell Recognition of the Major Group 5 Grass Pollen Allergen Phl P 5 The Journal of Allergy and Clinical Immunology. Mar, 2014 | Pubmed ID: 24182774 Crystal Structure of Human Poly(A) Polymerase Gamma Reveals a Conserved Catalytic Core for Canonical Poly(A) Polymerases Journal of Molecular Biology. Jan, 2014 | Pubmed ID: 24076191 Structure of Allergens and Structure Based Epitope Predictions Methods (San Diego, Calif.). Mar, 2014 | Pubmed ID: 23891546 Backbone Resonance Assignment of Alt a 1, a Unique β-barrel Protein and the Major Allergen of Alternaria Alternata Biomolecular NMR Assignments. Oct, 2014 | Pubmed ID: 23715812 Conjugative Type IV Secretion Systems in Gram-positive Bacteria Plasmid. Nov, 2013 | Pubmed ID: 24129002 TraG Encoded by the PIP501 Type IV Secretion System is a Two-domain Peptidoglycan-degrading Enzyme Essential for Conjugative Transfer Journal of Bacteriology. Oct, 2013 | Pubmed ID: 23913323 Characterization of Mutants of a Highly Cross-reactive Calcium-binding Protein from Brassica Pollen for Allergen-specific Immunotherapy Immunobiology. Sep, 2013 | Pubmed ID: 23790497 Crystal Structure and Immunologic Characterization of the Major Grass Pollen Allergen Phl P 4 The Journal of Allergy and Clinical Immunology. Sep, 2013 | Pubmed ID: 23683465 Identification of Der P 23, a Peritrophin-like Protein, As a New Major Dermatophagoides Pteronyssinus Allergen Associated with the Peritrophic Matrix of Mite Fecal Pellets Journal of Immunology (Baltimore, Md. : 1950). Apr, 2013 | Pubmed ID: 23460742 A Nonallergenic Birch Pollen Allergy Vaccine Consisting of Hepatitis PreS-fused Bet V 1 Peptides Focuses Blocking IgG Toward IgE Epitopes and Shifts Immune Responses to a Tolerogenic and Th1 Phenotype Journal of Immunology (Baltimore, Md. : 1950). Apr, 2013 | Pubmed ID: 23440415 Crystallization and Preliminary Structure Determination of the Transfer Protein TraM from the Gram-positive Conjugative Plasmid PIP501 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Feb, 2013 | Pubmed ID: 23385763 Different Modes of IgE Binding to CD23 Revealed with Major Birch Allergen, Bet V 1-specific Monoclonal IgE Immunology and Cell Biology. Feb, 2013 | Pubmed ID: 23229638 The 2.5 Å Structure of the Enterococcus Conjugation Protein TraM Resembles VirB8 Type IV Secretion Proteins The Journal of Biological Chemistry. Jan, 2013 | Pubmed ID: 23188825 S-adenosyl-L-homocysteine Hydrolase and Methylation Disorders: Yeast As a Model System Biochimica Et Biophysica Acta. Jan, 2013 | Pubmed ID: 23017368 Crystallization of Domains Involved in Self-assembly of the S-layer Protein SbsC Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Dec, 2012 | Pubmed ID: 23192035 Cleavage Factor Im is a Key Regulator of 3' UTR Length RNA Biology. Dec, 2012 | Pubmed ID: 23187700 Crystallization and First Data Collection of the Putative Transfer Protein TraN from the Gram-positive Conjugative Plasmid PIP501 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Nov, 2012 | Pubmed ID: 23143259 Genome-wide Analysis of Pre-mRNA 3' End Processing Reveals a Decisive Role of Human Cleavage Factor I in the Regulation of 3' UTR Length Cell Reports. Jun, 2012 | Pubmed ID: 22813749 Hypoallergenic Mutants of the Timothy Grass Pollen Allergen Phl P 5 Generated by Proline Mutations International Archives of Allergy and Immunology. 2012 | Pubmed ID: 22653364 Effect of Protamine on the Solubility and Deamidation of Human Growth Hormone International Journal of Pharmaceutics. May, 2012 | Pubmed ID: 22342332 Misdirected Antibody Responses Against an N-terminal Epitope on Human Rhinovirus VP1 As Explanation for Recurrent RV Infections FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Mar, 2012 | Pubmed ID: 22121050 The Structure of Bacterial S-layer Proteins Progress in Molecular Biology and Translational Science. 2011 | Pubmed ID: 21999995 Prediction of IgE-binding Epitopes by Means of Allergen Surface Comparison and Correlation to Cross-reactivity The Journal of Allergy and Clinical Immunology. Oct, 2011 | Pubmed ID: 21872913 PAPD5, a Noncanonical Poly(A) Polymerase with an Unusual RNA-binding Motif RNA (New York, N.Y.). Sep, 2011 | Pubmed ID: 21788334 Antiparasitic Compounds from Cupania Cinerea with Activities Against Plasmodium Falciparum and Trypanosoma Brucei Rhodesiense Journal of Natural Products. Apr, 2011 | Pubmed ID: 21438586 Altered IgE Epitope Presentation: A Model for Hypoallergenic Activity Revealed for Bet V 1 Trimer Molecular Immunology. Jan, 2011 | Pubmed ID: 21093057 Ground Vegetation Monitoring in Swiss Forests: Comparison of Survey Methods and Implications for Trend Assessments Environmental Monitoring and Assessment. Mar, 2011 | Pubmed ID: 21049285 Biophysical Characterization of Recombinant HIV-1 Subtype C Virus Infectivity Factor Amino Acids. Mar, 2011 | Pubmed ID: 20809132 Hypoallergenic Derivatives of the Major Birch Pollen Allergen Bet V 1 Obtained by Rational Sequence Reassembly The Journal of Allergy and Clinical Immunology. Nov, 2010 | Pubmed ID: 20638112 Arginine Methylation in Subunits of Mammalian Pre-mRNA Cleavage Factor I RNA (New York, N.Y.). Aug, 2010 | Pubmed ID: 20562214 Visualization of Clustered IgE Epitopes on Alpha-lactalbumin The Journal of Allergy and Clinical Immunology. Jun, 2010 | Pubmed ID: 20466413 Biochemical Characterization of Three Putative ATPases from a New Type IV Secretion System of Aeromonas Veronii Plasmid PAC3249A BMC Biochemistry. Feb, 2010 | Pubmed ID: 20144229 Towards the Structure of the C-terminal Part of the S-layer Protein SbsC Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Oct, 2009 | Pubmed ID: 19851018 Distinct Roles of Non-canonical Poly(A) Polymerases in RNA Metabolism PLoS Genetics. Jul, 2009 | Pubmed ID: 19593367 The High-molecular-mass Amylase (HMMA) of Geobacillus Stearothermophilus ATCC 12980 Interacts with the Cell Wall Components by Virtue of Three Specific Binding Regions Molecular Microbiology. Jun, 2009 | Pubmed ID: 19460092 Cloning, Expression, and Mapping of Allergenic Determinants of AlphaS1-casein, a Major Cow's Milk Allergen Journal of Immunology (Baltimore, Md. : 1950). Jun, 2009 | Pubmed ID: 19454699 The Role of the Putative 3' End Processing Endonuclease Ysh1p in MRNA and SnoRNA Synthesis RNA (New York, N.Y.). Dec, 2008 | Pubmed ID: 18971324 Disruption of Allergenic Activity of the Major Grass Pollen Allergen Phl P 2 by Reassembly As a Mosaic Protein Journal of Immunology (Baltimore, Md. : 1950). Oct, 2008 | Pubmed ID: 18802090 The Structure and Binding Behavior of the Bacterial Cell Surface Layer Protein SbsC Structure (London, England : 1993). Aug, 2008 | Pubmed ID: 18682224 Characterization of Folded Recombinant Der P 5, a Potential Diagnostic Marker Allergen for House Dust Mite Allergy International Archives of Allergy and Immunology. 2008 | Pubmed ID: 18520154 Crystal Structure of the 25 KDa Subunit of Human Cleavage Factor Im Nucleic Acids Research. Jun, 2008 | Pubmed ID: 18445629 Reduction of the in Vivo Allergenicity of Der P 2, the Major House-dust Mite Allergen, by Genetic Engineering Molecular Immunology. May, 2008 | Pubmed ID: 18295887 Three-dimensional Structure of the Cross-reactive Pollen Allergen Che a 3: Visualizing Cross-reactivity on the Molecular Surfaces of Weed, Grass, and Tree Pollen Allergens Journal of Immunology (Baltimore, Md. : 1950). Feb, 2008 | Pubmed ID: 18250440 Determinants of Substrate Specificity in RNA-dependent Nucleotidyl Transferases Biochimica Et Biophysica Acta. Apr, 2008 | Pubmed ID: 18177750 Mimotopes Identify Conformational B-cell Epitopes on the Two Major House Dust Mite Allergens Der P 1 and Der P 2 Molecular Immunology. Mar, 2008 | Pubmed ID: 17964653 A Hypoallergenic Vaccine Obtained by Tail-to-head Restructuring of Timothy Grass Pollen Profilin, Phl P 12, for the Treatment of Cross-sensitization to Profilin Journal of Immunology (Baltimore, Md. : 1950). Dec, 2007 | Pubmed ID: 18025208 RNA-specific Ribonucleotidyl Transferases RNA (New York, N.Y.). Nov, 2007 | Pubmed ID: 17872511 The Solution Structure of ParD, the Antidote of the ParDE Toxin Antitoxin Module, Provides the Structural Basis for DNA and Toxin Binding Protein Science : a Publication of the Protein Society. Aug, 2007 | Pubmed ID: 17656583 Genetic Engineering of the Major Timothy Grass Pollen Allergen, Phl P 6, to Reduce Allergenic Activity and Preserve Immunogenicity Journal of Immunology (Baltimore, Md. : 1950). Aug, 2007 | Pubmed ID: 17641039 Clinical Clarifications Requested Advances in Skin & Wound Care. Jun, 2007 | Pubmed ID: 17538250 A Recombinant Hypoallergenic Parvalbumin Mutant for Immunotherapy of IgE-mediated Fish Allergy Journal of Immunology (Baltimore, Md. : 1950). May, 2007 | Pubmed ID: 17475857 Splicing Factors Stimulate Polyadenylation Via USEs at Non-canonical 3' End Formation Signals The EMBO Journal. Jun, 2007 | Pubmed ID: 17464285 Bacterially Expressed and Optimized Recombinant Phl P 1 is Immunobiochemically Equivalent to Natural Phl P 1 Biochimica Et Biophysica Acta. Nov, 2006 | Pubmed ID: 17085087 An Interaction Between U2AF 65 and CF I(m) Links the Splicing and 3' End Processing Machineries The EMBO Journal. Oct, 2006 | Pubmed ID: 17024186 Structural Basis for Nucleic Acid and Toxin Recognition of the Bacterial Antitoxin CcdA Journal of Molecular Biology. Nov, 2006 | Pubmed ID: 17007877 Direct Interactions Between Subunits of CPSF and the U2 SnRNP Contribute to the Coupling of Pre-mRNA 3' End Processing and Splicing Molecular Cell. Jul, 2006 | Pubmed ID: 16857586 Cooking Birch Pollen-related Food: Divergent Consequences for IgE- and T Cell-mediated Reactivity in Vitro and in Vivo The Journal of Allergy and Clinical Immunology. Jul, 2006 | Pubmed ID: 16815162 The TraA Relaxase Autoregulates the Putative Type IV Secretion-like System Encoded by the Broad-host-range Streptococcus Agalactiae Plasmid PIP501 Microbiology (Reading, England). Mar, 2006 | Pubmed ID: 16514144 Bacterial Fermentation of Recombinant Major Wasp Allergen Antigen 5 Using Oxygen Limiting Growth Conditions Improves Yield and Quality of Inclusion Bodies Protein Expression and Purification. Jun, 2006 | Pubmed ID: 16495080 Crystallization and Preliminary Structure Determination of the Plant Food Allergen Pru Av 2 Acta Crystallographica. Section F, Structural Biology and Crystallization Communications. Feb, 2005 | Pubmed ID: 16510989 Dimerization of the Major Birch Pollen Allergen Bet V 1 is Important for Its in Vivo IgE-cross-linking Potential in Mice Journal of Immunology (Baltimore, Md. : 1950). Nov, 2005 | Pubmed ID: 16272319 WW Domains Provide a Platform for the Assembly of Multiprotein Networks Molecular and Cellular Biology. Aug, 2005 | Pubmed ID: 16055720 A Hybrid Molecule Resembling the Epitope Spectrum of Grass Pollen for Allergy Vaccination The Journal of Allergy and Clinical Immunology. May, 2005 | Pubmed ID: 15867859 A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality Control PLoS Biology. Jun, 2005 | Pubmed ID: 15828860 TraA and Its N-terminal Relaxase Domain of the Gram-positive Plasmid PIP501 Show Specific OriT Binding and Behave As Dimers in Solution The Biochemical Journal. Apr, 2005 | Pubmed ID: 15554903 Biochemical and Structural Insights into Substrate Binding and Catalytic Mechanism of Mammalian Poly(A) Polymerase Journal of Molecular Biology. Aug, 2004 | Pubmed ID: 15328606 Distinct Sequence Motifs Within the 68-kDa Subunit of Cleavage Factor Im Mediate RNA Binding, Protein-protein Interactions, and Subcellular Localization The Journal of Biological Chemistry. Aug, 2004 | Pubmed ID: 15169763 Functions for S. Cerevisiae Swd2p in 3' End Formation of Specific MRNAs and SnoRNAs and Global Histone 3 Lysine 4 Methylation RNA (New York, N.Y.). Jun, 2004 | Pubmed ID: 15146080 Sequence Motifs That Distinguish ATP(CTP):tRNA Nucleotidyl Transferases from Eubacterial Poly(A) Polymerases RNA (New York, N.Y.). Jun, 2004 | Pubmed ID: 15146073 Molecular Characterization of Polygalacturonases As Grass Pollen-specific Marker Allergens: Expulsion from Pollen Via Submicronic Respirable Particles Journal of Immunology (Baltimore, Md. : 1950). May, 2004 | Pubmed ID: 15128842 Generation of an Allergy Vaccine by Disruption of the Three-dimensional Structure of the Cross-reactive Calcium-binding Allergen, Phl P 7 Journal of Immunology (Baltimore, Md. : 1950). May, 2004 | Pubmed ID: 15100313 Ribavirin/interferon-alpha Sequential Treatment of Recurrent Hepatitis C After Liver Transplantation Transplant International : Official Journal of the European Society for Organ Transplantation. May, 2004 | Pubmed ID: 15060764 Circular Dichroism Analysis of Allergens Methods (San Diego, Calif.). Mar, 2004 | Pubmed ID: 14962758 Human Fip1 is a Subunit of CPSF That Binds to U-rich RNA Elements and Stimulates Poly(A) Polymerase The EMBO Journal. Feb, 2004 | Pubmed ID: 14749727 Crystallization and Preliminary Structure Determination of the C-terminal Truncated Domain of the S-layer Protein SbsC Acta Crystallographica. Section D, Biological Crystallography. Aug, 2003 | Pubmed ID: 12876353 The Role of the Yeast Cleavage and Polyadenylation Factor Subunit Ydh1p/Cft2p in Pre-mRNA 3'-end Formation Nucleic Acids Research. Jul, 2003 | Pubmed ID: 12853609 Formation of Disulfide Bonds and Homodimers of the Major Cat Allergen Fel D 1 Equivalent to the Natural Allergen by Expression in Escherichia Coli The Journal of Biological Chemistry. Oct, 2003 | Pubmed ID: 12732623 Independent Functions of Yeast Pcf11p in Pre-mRNA 3' End Processing and in Transcription Termination The EMBO Journal. May, 2003 | Pubmed ID: 12727883 Molecular Characterization of Recombinant T1, a Non-allergenic Periwinkle (Catharanthus Roseus) Protein, with Sequence Similarity to the Bet V 1 Plant Allergen Family The Biochemical Journal. Jul, 2003 | Pubmed ID: 12656672 X-filtering for a Range of Coupling Constants: Application to the Detection of Intermolecular NOEs Journal of Magnetic Resonance (San Diego, Calif. : 1997). Feb, 2003 | Pubmed ID: 12615149 Three-dimensional Structure of the Panallergen Phl P 7 International Archives of Allergy and Immunology. Jan, 2003 | Pubmed ID: 12576729 RNA Editing by Adenosine Deaminases Generates RNA and Protein Diversity Biochimie. Aug, 2002 | Pubmed ID: 12457566 A Role for SSU72 in Balancing RNA Polymerase II Transcription Elongation and Termination Molecular Cell. Nov, 2002 | Pubmed ID: 12453421 Characterization of a Novel Isoform of Alpha-nascent Polypeptide-associated Complex As IgE-defined Autoantigen The Journal of Investigative Dermatology. Oct, 2002 | Pubmed ID: 12406326 The Cross-reactive Calcium-binding Pollen Allergen, Phl P 7, Reveals a Novel Dimer Assembly The EMBO Journal. Oct, 2002 | Pubmed ID: 12356717 Gene Regulation: Reviving the Message Nature. Sep, 2002 | Pubmed ID: 12239557 Combination Vaccines for the Treatment of Grass Pollen Allergy Consisting of Genetically Engineered Hybrid Molecules with Increased Immunogenicity FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology. Aug, 2002 | Pubmed ID: 12154002 Yhh1p/Cft1p Directly Links Poly(A) Site Recognition and RNA Polymerase II Transcription Termination The EMBO Journal. Aug, 2002 | Pubmed ID: 12145212 TadA, an Essential TRNA-specific Adenosine Deaminase from Escherichia Coli The EMBO Journal. Jul, 2002 | Pubmed ID: 12110595 Recombinant Carp Parvalbumin, the Major Cross-reactive Fish Allergen: a Tool for Diagnosis and Therapy of Fish Allergy Journal of Immunology (Baltimore, Md. : 1950). May, 2002 | Pubmed ID: 11971005 Functional Analysis of Yeast SnoRNA and SnRNA 3'-end Formation Mediated by Uncoupling of Cleavage and Polyadenylation Molecular and Cellular Biology. Mar, 2002 | Pubmed ID: 11839805 Mutants of the Major Ryegrass Pollen Allergen, Lol P 5, with Reduced IgE-binding Capacity: Candidates for Grass Pollen-specific Immunotherapy European Journal of Immunology. Jan, 2002 | Pubmed ID: 11782018 The Anti-toxin ParD of Plasmid RK2 Consists of Two Structurally Distinct Moieties and Belongs to the Ribbon-helix-helix Family of DNA-binding Proteins The Biochemical Journal. Jan, 2002 | Pubmed ID: 11743881 3' End Sequencing Library Preparation with A-seq2 Georges Martin1, Ralf Schmidt1, Andreas J. Gruber1, Souvik Ghosh1, Walter Keller1, Mihaela Zavolan1,2 1Computational and Systems Biology, Biozentrum, University of Basel, 2Swiss Institute of Bioinformatics, Biozentrum, University of Basel JoVE 56129 생물학
3' End Sequencing Library Preparation with A-seq2 Georges Martin1, Ralf Schmidt1, Andreas J. Gruber1, Souvik Ghosh1, Walter Keller1, Mihaela Zavolan1,2 1Computational and Systems Biology, Biozentrum, University of Basel, 2Swiss Institute of Bioinformatics, Biozentrum, University of Basel JoVE 56129 생물학