Novel Protocol for Simultaneous Fluorescence and Ultrastructure Preservation

January 12, 2024

Epon Post 嵌入相关光和电子显微镜

用于 Epon 后嵌入相关光和电子显微镜（CLEM）的超薄切片

创建CLEM导航图和制备电子显微镜的超薄切片

用于 Epon 后嵌入相关光和电子显微镜的小鼠大脑切片明场和荧光图像的图像分析

荧光显微镜图像与电子显微镜图像的配准，用于相关光和电子显微镜

Shuyuan Wang*¹, Haiyan Xiong*^1,2, Qiyuan Chang*^1,3, Xudong Zhuang*^4,5,6, Yaochen Wu³, Xinrui Wang^5,6, Congxian Wu, Zhifei Fu^7,8

¹Public Technology Service Center,Fujian Medical University, ²The School of Pharmacy,Fujian Medical University, ³The School of Basic Medical Sciences,Fujian Medical University, ⁴College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics,Fujian Medical University, ⁵Medical Research Center,Fujian Maternity and Child Health Hospital, ⁶NHC Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate,Fujian Maternity and Child Health Hospital, ⁷Institute of Neuroscience,Fujian Medical University, ⁸Fujian Key Laboratory of Molecular Neurology,Fujian Medical University

Summary

我们提出了使用称为 mScarlet 的荧光蛋白进行 Epon 嵌入后相关光学和电子显微镜的详细方案。这种方法可以同时保持荧光和超微结构。该技术适用于各种生物应用。

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