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Showing 1 - 12 of 32 videos for "Targeted Transfection"

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Journal (Bioengineering)

Production of Recombinant AAV by Transient Transfection

Miguel M. Lopes*^1,2,3, Sara M. Lopes*^1,2,3, Rafael Baganha^2,4, Carina Henriques^2,5, Ana C. Silva^2,3, Diana D. Lobo^2,3, Luísa Cortes^2,3,6, Luís Pereira de Almeida*^1,2,4,5, Rui Jorge Nobre*^1,2,3,4

¹CNC-UC - Center for Neuroscience and Cell Biology,University of Coimbra, ²CIBB - Center for Innovative Biomedicine and Biotechnology,University of Coimbra, ³IIIUC - Institute for Interdisciplinary Research,University of Coimbra, ⁴ViraVector - Viral Vectors for Gene Transfer Core Facility,University of Coimbra, ⁵FFUC - Faculty of Pharmacy,University of Coimbra, ⁶MICC-CNC - Microscopy Imaging Center of Coimbra - CNC,University of Coimbra

Journal (Biology)

Preclearing of Transfected HEK293 Protein with the Protein G Gel and Capturing Protein Complexes Using Epitope Tag Affinity Gel

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Journal (Biology)

Cell Lysis of Transfected HEK293 Cells and Protein Sample Preparation for Immunoprecipitation

Hiroko Shinjo, Gaku Nagano, Shogo Ishii, Natsumi Himeno, Yutaro Yamamoto, Junji Sagawa, Ryuta Baba, Gentaro Egusa, Noboru Hattori, Haruya Ohno

¹Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

Journal (Neuroscience)

HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production

Kimberly L. Pietersz, Paul J.H. Nijhuis, Matthijs H.M. Klunder, Joëlle van den Herik, Barbara Hobo, Fred de Winter, Joost Verhaagen²

¹Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), ²Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

Journal (Bioengineering)

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Methods Collections

Demonstration of Self-Assembled Cell Sheet Culture and Manual Generation of a 3D Tendon/Ligament-Like Organoid by using Human Dermal Fibroblasts

Ana Luísa Graça, Niklas Kroner-Weigl, Viviana Reyes Alcaraz, Sigrid Müller-Deubert, Maximilian Rudert, Denitsa Docheva

¹Department of Musculoskeletal Tissue Regeneration, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg, ²Laboratory for Experimental Trauma Surgery, Department of Trauma Surgery,University Hospital Regensburg, ³Department of Orthopaedics, Orthopaedic Hospital König-Ludwig-Haus,University of Würzburg

Journal (Biology)

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Methods Collections

Polymerase Chain Reaction and Dot-Blot Hybridization for Leptospira Detection in Water Samples

Eduardo Delgadillo-Tellez, Beatriz Zavaleta-Villa, Daniel Atilano-López, Rigoberto Hernández-Castro, Angélica Olivo-Díaz, Erika Margarita Carrillo-Casas

¹Department of Molecular Biology and Histocompatibility,General Hospital “Dr. Manuel Gea González”, ²Diagnostic Laboratory-Bacteriology, Leptospira Section, Department of Microbiology and Immunology, Veterinary Medicine Faculty,UNAM, ³Department of Ecology of Pathogens,General Hospital “Dr. Manuel Gea González”

Journal (Neuroscience)

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Methods Collections

An Experiment Using Functional Near-Infrared Spectroscopy and Robot-Assisted Multi-Joint Pointing Movements of the Lower Limb

João Ricardo Sato, Anna Carolyna Gianlorenço, Elayne Borges Fernandes, Thalita Frigo da Rocha, Antonio Massato Makiyama*³, Laura Dipietro*⁴

¹Center of Mathematics, Computing and Cognition,Universidade Federal do ABC, ²Department of the Physical Therapy,Federal University of Sao Carlos, ³Vivax Ltda, ⁴Highland Instruments

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