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Journal
/
Neuroscience
/
Simplified and Efficient AAV Production Using HEK293 Suspension Culture
/
HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production
HEK293 Suspension Cell Cultivation and Viral Vector Harvesting for Virus Production
Simplified and Efficient AAV Production Using HEK293 Suspension Culture
DOI:
10.3791/201530-v
•
03:26 min
•
April 26, 2024
•
Kimberly L. Pietersz
,
Paul J.H. Nijhuis
,
Matthijs H.M. Klunder
,
Joëlle van den Herik
,
Barbara Hobo
,
Fred de Winter
,
Joost Verhaagen
2
1
Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience
,
Royal Netherlands Academy of Arts and Sciences (KNAW)
,
2
Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience
,
Vrije Universiteit Amsterdam
Tags
Keywords: HEK293
Suspension Cell Cultivation
Viral Vector Harvesting
Virus Production
Cell Culture
Transfection
Plasmids
Cell Lysis
PEG Precipitation
Downstream Processing
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