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Simplified and Efficient AAV Production Using HEK293 Suspension Culture
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Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production
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JoVE Journal Neuroscience
Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

Iodixanol Gradient Purification of Transfected HEK293 Cell Lysates for Concentrated Adeno-Associated Virus Production

Simplified and Efficient AAV Production Using HEK293 Suspension Culture

DOI:
10.3791/201531-v

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03:36 min

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April 26, 2024

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, , , , , ,
1Laboratory for Regeneration of Sensorimotor Systems, Netherlands Institute for Neuroscience,Royal Netherlands Academy of Arts and Sciences (KNAW), 2Centre for Neurogenomics and Cognitive Research, Amsterdam Neuroscience,Vrije Universiteit Amsterdam

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Keywords: Iodixanol GradientHEK293 Cell LysatesAdeno-associated Virus ProductionPEG PrecipitationDNase I TreatmentUltracentrifugationVirus ExtractionBuffer ExchangeCentrifugal Filtration

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