大会，调整和使用蛋白成像Apertureless近场红外显微镜
Summary
描述成像蛋白质总量的大会，是一个近场红外显微镜。
Abstract
本文旨在指导读者在超越衍射极限的成像的红外线近场显微镜的装配和操作。 apertureless近场显微镜是一种光散射式仪器，可提供大约20 nm分辨率的红外光谱。一个组件的完整列表和使用一步一步的协议提供。大会和仪器调谐常见的错误进行了讨论。一个有代表性的数据集，显示了二级结构的淀粉样原纤维。
Protocol
背景： Apertureless探头近场红外显微镜提供高空间分辨率的成像。它是一个相对较新的技术，其中一个事件的红外光束被锋利的原子力显微镜（AFM）在接近样品的悬臂共振频率振荡提示分散。红外探测器收集的散射光，并在这个共振频率及其谐波解调。这样一来，其余样品表面上的聚焦激光束事件的背景分散可以降低一个远远超出了光的衍射极限的空间分辨率可以达到获得与纳…
Acknowledgements
我们非常感谢美国国家科学基金会，NSERC，美国国立卫生研究院和ONR。
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Cite This Article
Paulite, M., Fakhraai, Z., Akhremitchev, B. B., Mueller, K., Walker, G. C. Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging. J. Vis. Exp. (33), e1581, doi:10.3791/1581 (2009).