Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts - JoVE Wordpress Development

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Tümör Organoidleri ve Primer Fibroblastlar Oluşturmak için Taze Primer Tümör Dokusu İşleme

Published: May 26, 2023

doi:

Jesús Frutos Díaz-Alejo*^1,2,3,4, Simon April-Monn*⁵, Marina Cihova, Verona Buocikova, Jorge Villalón López³, Maria Urbanova, Carmen G. Lechuga, Miroslav Tomas⁸, Peter Dubovan⁸, Bárbara Luna Sánchez, Sonia Camaño Páez, Alfonso Sanjuanbenito⁹, Eduardo Lobo, Estefanía Romio de la Heras, Carmen Guerra⁷, Carolina de la Pinta, Emma Barreto Melian², Mercedes Rodríguez Garrote², Alfredo Carrato^2,4, Laura Ruiz-Cañas^12,13, Bruno Sainz, Jr.^3,12,13, Ana Torres, Bozena Smolkova, Julie Earl^2,3

¹Molecular Epidemiology and Predictive Tumor Markers Group, Area 3,Ramón y Cajal Health Research Institute (IRYCIS), ²The Biomedical Research Network in Cancer (CIBERONC), ³Biobank and Biomodels Platform (PT20/0045), ISCIII research and development platforms in biomedicine and health sciences, BioBank Hospital Ramón y Cajal-IRYCIS, Spanish National Biobanks Network (ISCIII Biobank Register No. B.0000678),Ramón y Cajal Health Research Institute (IRYCIS), ⁴Faculty of Medicine,University of Alcalá de Henares, ⁵Institute of Tissue Medicine and Pathology,University of Bern, ⁶Department of Molecular Oncology, Cancer Research Institute,Biomedical Research Center of the Slovak Academy of Sciences, ⁷Experimental Oncology, Molecular Oncology Program,Centro Nacional de Investigaciones Oncológicas (CNIO), ⁸Department of Surgical Oncology, National Cancer Institute,Slovak Medical University, ⁹Pancreatic and Biliopancreatic Surgery Unit,Hospital Universitario Ramón y Cajal, ¹⁰Department of Pathology,Hospital Universitario Ramón y Cajal, ¹¹Department of Radiation Oncology,Hospital Universitario Ramón y Cajal, ¹²Department of Cancer,Instituto de Investigaciones Biomédicas “Alberto Sols” (IIBM), ¹³Cancer Stem Cell and Fibroinflammatory Group, Chronic Diseases and Cancer, Area 3,IRYCIS

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Keywords: Tumor Organoids Primary Fibroblasts DMEM/F-12 Media PBS Digestion Media Trypsin Cell Filtration Cell Centrifugation Red Blood Cell Lysis Cell Dissociation Cell Resuspension Membrane Matrix

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Fresh Primary Tumor Tissue Processing to Establish Tumor Organoids and Primary Fibroblasts. J. Vis. Exp. (Pending Publication), e200204, doi:10.3791/200204 (2023).

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