Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs)

从小鼠骨髓造血干细胞和祖细胞（HSPC）纯化细胞核

Published: December 22, 2023

doi:

10.3791/200693

Carolina Moraes Cabé, Sophie Novault, Ana Jeemin Choi, Valerie Seffer, Laura Barrio Cano, Valentina Libri³, Milena Hasan

¹Cytometry and Biomarkers UTechS, C2RT,Institut Pasteur, Université Paris Cité, ²Microenvironment and Immunity Unit,Institut Pasteur, Université Paris Cité, INSERM U1224, ³Istituto Superiore di Sanità

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Keywords: Nuclei Purification Mouse Bone Marrow Hematopoietic Stem And Progenitor Cells (HSPCs)ACK Lysing Buffer Cell Strainer FACS Buffer Cell Counting Cell Staining Flow Cytometry Cell Sorting Collection Medium PBS BSA

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Purification of Nuclei from Mouse Bone Marrow Hematopoietic Stem and Progenitor Cells (HSPCs). J. Vis. Exp. (Pending Publication), e200693, doi:10.3791/200693 (2023).

从小鼠骨髓造血干细胞和祖细胞 （HSPC） 纯化细胞核

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从小鼠骨髓造血干细胞和祖细胞（HSPC）纯化细胞核