Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion

使用悬滴蒸气扩散法结晶脂质二聚体ABCG5/G8蛋白

Published: August 25, 2023

doi:

10.3791/200737

Sarah Wazir, Danny Farhat, Mahalashmi Srinivasan², Jyh-Yeuan Lee

¹Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine,University of Ottawa, ²Department of Biochemistry, Faculty of Medicine and Dentistry,University of Alberta

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Keywords: Crystallization Heterodimeric ABCG5/G8 Protein Lipidic Bicelle Hanging Drop Vapor Diffusion Bicelle Stock Solution DMPC CHAPSO Protein-bicelle Mixture Crystallization Reservoir Solution Crystal Growth Sodium Malate Cryo Loops Data Collection

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Crystallization of Heterodimeric ABCG5/G8 Protein in Lipidic Bicelle Using Hanging Drop Vapor Diffusion. J. Vis. Exp. (Pending Publication), e200737, doi:10.3791/200737 (2023).

使用悬滴蒸气扩散法结晶脂质二聚体ABCG5/G8蛋白

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