Cleaning and Reusing Polycarbonate Ultracentrifuge Tubes for Sustainable Extracellular Vesicle Isolation - JoVE

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Cleaning and Reusing Polycarbonate Ultracentrifuge Tubes for Sustainable Extracellular Vesicle Isolation

Published: March 08, 2024

doi:

Rachel M. E. Cahalane*^1,2, Mandy E. Turner*¹, Cassandra L. Clift, Mark C. Blaser, Gabrielle Bogut, Sydney Levy, Taku Kasai, Tom A. P. Driedonks⁴, Esther N. M. Nolte-‘t Hoen, Masanori Aikawa⁵, Sasha A. Singh⁵, Elena Aikawa⁵

¹Center for Interdisciplinary Cardiovascular Sciences, Cardiovascular Division, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School, ²Mechanobiology and Medical Device Research Group (MMDRG), Biomedical Engineering, College of Science and Engineering,University of Galway, ³Department of CDL Research,University Medical Center Utrecht, ⁴Department of Biomolecular Health Sciences, Faculty of Veterinary Medicine,Utrecht University, ⁵Center for Excellence in Vascular Biology, Cardiovascular Division, Department of Medicine,Brigham and Women’s Hospital, Harvard Medical School

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Keywords: Polycarbonate Tubes Ultracentrifuge Extracellular Vesicle Isolation SDS Cotton Swab Tap Water Demineralized Water Ethanol Sustainable Reuse

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Cleaning and Reusing Polycarbonate Ultracentrifuge Tubes for Sustainable Extracellular Vesicle Isolation. J. Vis. Exp. (Pending Publication), e201569, doi:10.3791/201569 (2024).

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