The video explains a stepwise surgical method for orthotopic implantation of a tumor fragment from a cancerous donor to the pancreas of a non-cancerous recipient mice for development of pancreatic cancer. Orthotopically implanted PDAC could recapture the tissue microenvironment of human pancreatic cancers than conventional subcutaneous (SC) models more accurately.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation for Tumor Transplantation Animal Housing Obtain generic C57BL/6 mice from a commercial breeding vendor. House mice (5) in individual ventilated cages at the following conditions: Temperature: 20–26 °C; humidity 30–70%; lighting cycle: 12 h light and 12…