Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount
Published: April 30, 2023
Abstract
Source: Yoneda, M. et al. A Simple Device to Rapidly Prepare Whole Mounts of the Mouse Intestine. J. Vis. Exp. (2015)
In this video, we demonstrate whole mount preparation of the mouse intestine using a simple gut-incising device. The resulting mounts are of superior and uniform quality and can be used for histological analyses.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Device Machine the main portion of the base of the device from out of a solid block of high-density acetyl resin, as described in this manuscript. At each end, create 3 mm high-elevated strips and part machine out of these 2.5 mm grooves to take the rods (see Figure 1 and …
Representative Results
Figure 1. Photos of the original device. The original device was constructed from several pieces of metal, prepared by hand. (A) The device assembled. (B) The top of the device has been removed to show the rods in position. <img alt="Figure 2" class="xfigimg" src…
Disclosures
The authors have nothing to disclose.
Materials
| Delrin | Ryan Plastics, Earls Barton, UK | High-density acetal resin similar material would suffice | |
| Duralumin plate | Metal Supplies Ltd, Park Road,Dukinfield. SK16 5LP UK | Aluminium allow dating back to 1909 so alterative suppliers are available | |
| Finished device(s) ready for use | Contact Dr Goodlad r.goodlad@imperial.ac.uk | Contact r.goodlad@imperial.ac.uk for supply details |
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Cite This Article
Whole Mount Preparation of Mouse Intestine: Using a Simple Gut-incising Device to Prepare Whole Mount. J. Vis. Exp. (Pending Publication), e20424, doi: (2023).