Murine Sciatic Nerve Model of Perineural Invasion or PNI: Extraction and Processing of Sciatic Nerve to Understand Invasion by Cancer Cells
Published: April 30, 2023
Abstract
Source: Deborde, S. et al. An In Vivo Murine Sciatic Nerve Model of Perineural Invasion. J. Vis. Exp. (2018)
In this video, we describe the procedure to establish a murine model of perineural invasion by injecting cancer cells into the sciatic nerve. The model can help gain an understanding of the magnitude of nerve invasion for subsequent assessment of anti-cancer drugs.
Protocol
All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Sciatic Nerve Extraction Take mice whose sciatic nerves are previously injected with cancer cells and euthanize them with CO2. Place the mouse on its ventral side and stabilize the distal limbs using pins. Remove the skin on the dorsal side of the injected limb and torso. Using blunt disse…
Disclosures
The authors have nothing to disclose.
Materials
| Mouse | Number and age variable depending on experimental needs | ||
| Sterile surgical tools (scissors and forceps) | |||
| Tissue-Tek O.C.T. Compound | VWR | 25608-930 | |
| Tissue-Tek Cryomold Molds | VWR | 25608-916 |
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Cite This Article
Murine Sciatic Nerve Model of Perineural Invasion or PNI: Extraction and Processing of Sciatic Nerve to Understand Invasion by Cancer Cells. J. Vis. Exp. (Pending Publication), e20687, doi: (2023).