Intranasal Delivery of Therapeutic Neural Stem Cells (NSCs) in Murine Model: A Procedure for Tumor-targeted Delivery of Oncolytic Virus Loaded NSCs into Brain Tumor Bearing Mouse Model

Abstract

Source: Yu, D. et al. Intranasal Delivery of Therapeutic Stem Cells to Glioblastoma in a Mouse Model. J. Vis. Exp. (2017)

This video demonstrates the intranasal delivery of oncolytic virus loaded therapeutic neural stem cells into a brain-tumor bearing mouse model. The non-invasive intranasal administration of stem cells bypasses the blood brain barrier, facilitating their delivery to the tumor site in the brain for therapeutic applications.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Hypoxic Preconditioning of Human NSCs (Figure 1A) Plate cultured NSCs in T75 culture flasks until 90% confluency is reached. Place cell culture flasks inside a humidified CO2 chamber/incubator with maintained 1% O2 levels for 24 h, via N2&#1…

Representative Results

Figure 1: A flow chart for general stem cell preparation procedures involved prior to intranasal delivery. (A–C) Hypoxic pretreatment or genetic modifications of stem cells to overexpress CXCR4. (B–D) Washing, enzymatic dissociation, cell collection, and counting steps are performed for subsequent loading of stem cells with therapeutic or diagnostic c…

Materials

Cell culture supplies (Plastics)	ThermoFisher Scientific	Varies	Replaceable with any source
Legend Micro 21R Refrigerated Microcentrifuge	ThermoFisher Scientific	75002490	Replaceable with any source
Bench centrifuge Sorvall ST16R	ThermoFisher Scientific	75004240	Replaceable with any source