Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections

Published: September 29, 2023

Abstract

Source: Du, Y. W. et al., Effects of Taste Signaling Protein Abolishment on Gut Inflammation in an Inflammatory Bowel Disease Mouse Model. J. Vis. Exp. (2018)

This video demonstrates the assessment of chemical-induced colitis via immunohistochemical staining of mouse colon tissue. In a stained tissue section, the presence of a large number of immune-responsive cells in the damaged tissue represents the infiltration of immune cells during colitis.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of Mice and DSS Keep the knockout (α-gustducin-/-) mice and age-, gender-, and body weight-matched wild-type control (α-gustducin+/+) C57BL/6 mice individually in clean cages. NOTE: The knockout mice have been backcrossed with C57BL/6 mice for over 20 g…

Disclosures

The authors have nothing to disclose.

Materials

Antibody
CD45 BD Biosciences 550539
CD3 BD Biosciences 555273
B220 BD Biosciences 550286
CD11b BD Biosciences 550282
Ly6G BD Biosciences 551459
Reagent
Dextran Sulfate Sodium Salt (DSS) MP Biomedicals 2160110
Streptavidin-HRP complex BD Pharmingen 551011
Phosphate-Buffered Saline (PBS) Sangon Biotech B548117
BD 10 ml Syringe BD Biosciences 309604
Instruments and equipment
Balance
Scissors 
Forceps
Staining jars
Software
Imag-Pro Plus  Media Cybernetics, Inc. 

Tags

Play Video

Cite This Article
Assessment of Chemical-Induced Colitis via Immunohistochemical Analysis of Colon Tissue Sections. J. Vis. Exp. (Pending Publication), e21644, doi: (2023).

View Video