A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves

Published: October 31, 2023

Abstract

Source: Zhang, M., et al. The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea. J. Vis. Exp. (2018).

This video demonstrates a technique to establish the pathogenic fungus Magnaporthe grisea infection in rice plant leaves. The fungal conidia suspension is sprayed on sections of rice leaf, and the disease progression is assessed by scoring the formation of necrotic lesions.

Protocol

1. Spray Inoculation with a Suspension of M. grisea Conidia Fungal culture for M.grisea Prepare the oatmeal tomato agar (OTA) culture medium for fungal strains. Weigh 30 – 50 g of oatmeal, add this to 800 mL of distilled/deionized water (ddH2O), and boil the mixture for 30 min in the electric pot. Filter the boiled oatmeal juice into the beaker through a piece of gauze. Add 150 mL of tomato juice and 20…

Disclosures

The authors have nothing to disclose.

Materials

Agar AOBOX Biotechnology (China) 01-023
Filter paper GE Healthcare brand(Sweden) 10311387
50-mL tube CORNING(Amercia) 430290
Centrifuge Eppendorf(Amercia) 5804R
Tween-20 Coolaber (China) CT11551-100ml
Culture dish Thermofisher(Amercia) 150326
0.5-5 mL pipette Eppendorf 4920000105
100-1000uL pipette Eppendorf 4920000083
Vacuum pump Leybold D25B
Incubator MEMMERT PYX313
Inoculation ring Greiner Bio One 731175

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Cite This Article
A Spray Inoculation Technique to Establish Fungal Infection in Rice Plant Leaves. J. Vis. Exp. (Pending Publication), e21754, doi: (2023).

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