Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model

Published: November 30, 2023

Abstract

Source: Ares, G. J., et al. A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis. J. Vis. Exp.(2019).

This video demonstrates the development of necrotizing enterocolitis disease in a human-derived neonatal enteroid model induced by lipopolysaccharides treatment. This model helps to study intestinal pathophysiology.

Protocol

All procedures involving human participants have been performed in compliance with the institutional, national, and international guidelines for human welfare and have been reviewed by the local institutional review board. 1. Reagent Preparation Prepare Culture Media stock solution for whole tissue collection: 1 L of Dulbecco's Modified Eagle Medium (DMEM), 110 mL of Fetal Bovine Serum (FBS), 11 mL of Penicillin-Streptomycin (final concentration 1%), and 1.1 mL…

Disclosures

The authors have nothing to disclose.

Materials

4% Paraformaldehyde ThermoFisher AAJ19943K2
Basement Membrane Matrix (Matrigel) Corning CB-40230C
DMEM/F-12 ThermoFisher MT-16-405-CV
Dulbecco's Modified Eagle Medium (DMEM) ThermoFisher 11-965-118
Dulbecco's Phosphate-Buffered Saline (DPBS) ThermoFisher 14190-144
Epidermal Growth Factor (EGF) Sigma E9644-.2MG
Ethylenediaminetetraacetic acid (EDTA) Sigma EDS-500G
Fetal Bovine Serum (FBS) Gemini Bio-Pro 100-125
Phosphate Buffered Saline (PBS) Sigma P5368-5X10PAK
RPMI 1640 Medium Invitrogen 11875093
Tissue Processing Gel (Histogel) ThermoFisher 22-110-678
Lipopolysaccharide (LPS) Sigma L2630-25MG
Gentamicin Sigma G5013-1G

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Cite This Article
Induction of Necrotizing Enterocolitis in Human Epithelial Enteroid Model. J. Vis. Exp. (Pending Publication), e21803, doi: (2023).

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