Convection-Enhanced Delivery of Antibodies into a Mouse Brain

Published: March 29, 2024

Abstract

Source: Beffinger, M., et al. Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery. J. Vis. Exp. (2019).

This video demonstrates a convection-enhanced delivery (CED) technique for antibody delivery into the mouse brain. The skull of an anesthetized mouse is exposed, and a burr hole is created at the target area for antibody delivery. A step catheter containing antibodies is inserted at the target site, and via an infusion pump, a pressure gradient is created to deliver antibodies at a controlled rate.

Protocol

All procedures involving animal models have been reviewed by the local institutional animal care committee and the JoVE veterinary review board. 1. Preparation of the Step Catheters Preparation of a fused silica tubing for the step of the catheter Cut the fused silica capillary with an inner diameter of 0.1 mm and wall thickness of 0.0325 mm tubing to a length of 30 mm. Examine the tubing for cracks and heat polish the ends u…

Representative Results

Figure 1: A schematic drawing depicting the application area of the adhesive. The upper 10 mm of the fused silica tubing is inserted into the metal needle. Apply the glue on the 10 mm tubing starting from the tip of the metal needle.

Disclosures

The authors have nothing to disclose.

Materials

10 μL syringe Hamilton 7635-01
27 G blunt end needle Hamilton 7762-01
Agarose Promega V3121
Atipamezol Janssen
Bone wax Braun 1029754
Buprenorphine Indivior Schweiz AG
Carprofen Pfizer AG
Dental drill bits, steel, size ISO 009 Hager & Meisinger 1RF009
Ethanol 100% Reuss-Chemie AG 179-VL03K-/1
Fentanyl Helvepharm AG
FITC-Dextran, 2000 kDa Sigma Aldrich FD2000S
Flumazenil Labatec Pharma AG
Formaldehyde Sigma Aldrich F8775-500ML
High viscosity cyanoacrylate glue Migros
Iodine solution Mundipharma
Medetomidin Orion Pharma AG
Microforge Narishige MF-900
Midazolam Roche Pharma AG
Ophthalmic ointment Bausch + Lomb Vitamin A Blache
PBS ThermoFischer Scientific 10010023
Polyclonal goat anti-rat IgG (H+L) antibody coupled with Alexa Fluor 647 Jackson Immuno
Scalpels Braun BB518
Silica tubing internal diameter 0.1 mm, wall thickness of 0.0325 mm Postnova Z-FSS-100165
Stereotactic frame for mice Stoelting 51615
Stereotactic robot Neurostar Drill and Injection Robot
Sucrose Sigma Aldrich S0389-500G
Topical tissue adhesive Zoetis GLUture
Trypan blue ThermoFischer Scientific 15250061
Water Bichsel 1000004

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Cite This Article
Convection-Enhanced Delivery of Antibodies into a Mouse Brain. J. Vis. Exp. (Pending Publication), e22092, doi: (2024).

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