Diblock polymeric नैनोकणों के Nanoprecipitation तकनीक के माध्यम से निरूपण
Summary
यह लेख एक nanoprecipitation बहुलक आधारित diblock सह – पॉलिमर का उपयोग नैनोकणों synthesize विधि का वर्णन करता है. हम diblock सह पॉलिमर, nanoprecipitation तकनीक, और संभावित अनुप्रयोगों के संश्लेषण पर चर्चा करेंगे.
Abstract
Nanotechnology is a relatively new branch of science that involves harnessing the unique properties of particles that are nanometers in scale (nanoparticles). Nanoparticles can be engineered in a precise fashion where their size, composition and surface chemistry can be carefully controlled. This enables unprecedented freedom to modify some of the fundamental properties of their cargo, such as solubility, diffusivity, biodistribution, release characteristics and immunogenicity. Since their inception, nanoparticles have been utilized in many areas of science and medicine, including drug delivery, imaging, and cell biology1-4. However, it has not been fully utilized outside of “nanotechnology laboratories” due to perceived technical barrier. In this article, we describe a simple method to synthesize a polymer based nanoparticle platform that has a wide range of potential applications.
The first step is to synthesize a diblock co-polymer that has both a hydrophobic domain and hydrophilic domain. Using PLGA and PEG as model polymers, we described a conjugation reaction using EDC/NHS chemistry5 (Fig 1). We also discuss the polymer purification process. The synthesized diblock co-polymer can self-assemble into nanoparticles in the nanoprecipitation process through hydrophobic-hydrophilic interactions.
The described polymer nanoparticle is very versatile. The hydrophobic core of the nanoparticle can be utilized to carry poorly soluble drugs for drug delivery experiments6. Furthermore, the nanoparticles can overcome the problem of toxic solvents for poorly soluble molecular biology reagents, such as wortmannin, which requires a solvent like DMSO. However, DMSO can be toxic to cells and interfere with the experiment. These poorly soluble drugs and reagents can be effectively delivered using polymer nanoparticles with minimal toxicity. Polymer nanoparticles can also be loaded with fluorescent dye and utilized for intracellular trafficking studies. Lastly, these polymer nanoparticles can be conjugated to targeting ligands through surface PEG. Such targeted nanoparticles can be utilized to label specific epitopes on or in cells7-10.
Protocol
Discussion
nanoprecipitation सह पॉलिमर – diblock पद्धति का उपयोग करके एक सरल, तेजी से विधि polymeric नैनोकणों इंजीनियर का प्रतिनिधित्व करता है. परिणामस्वरूप नैनोकणों एक hydrophobic कोर, जो खराब घुलनशील यौगिकों के वितरण के लिए उपयोग किया जा सक?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
इस काम के कैंसर नैनो उत्कृष्टता पायलट अनुदान, विश्वविद्यालय के कैंसर रिसर्च फंड और राष्ट्रीय स्वास्थ्य संस्थान K-12 कैरियर विकास पुरस्कार के लिए केरोलिना केंद्र के खिलाफ Golfers द्वारा वित्त पोषित किया गया था.
Materials
| Reagent | Company | Catalogue Number | Comments |
| EDC | Thermo Scientific | 22980 | Conjugation Reagent |
| NHS | Thermo Scientific | 24500 | Conjugation Reagent |
| amine-PEG-carboxylate | Laysan Bio Inc. | Nh2-PEG-CM-5000 | Polymer (Can use any PEG MW, 5000 is listed here) |
| PLGA-carbxylate | Lactel | B6013-2 | Polymer |
| Dichloromethane (DCM) | Sigma-Aldrich | 34856 | Solvent |
| Acetonitrile >99% purity | Sigma-Aldrich | 34851 | Solvent |
| Methanol >99% purity | Sigma-Aldrich | 34860 | Wash |
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