5’-AAG GGA CCT GCB GCA GGT CCC TTG ATA CGA TTC TA -3’ ; B – Biotin-dT; 100 pmol/μL stock in ddH2O
Adapter Oligonucleotide
IDT DNA
5’-TAG AAT CGT ATC-3’; 100 pmol/μL stock in ddH2O
1 M Tris-HCl, PH 7.4
Lonza
51237
TSA, Fluorescein System
PerkinElmer
NEL701A001KT
1 M NaCl
DMSO
Sigma
Tween-20
Sigma
P9416-50ML
Vectashield with DAPI
Vector Laboratories
H-1200
Coverslips
22x22mm or 22x40mm glass or plastic coverslips. Plastic coverslips are preferable during the reaction, as they are easier to remove from the section.
Rat Brain Sections
Rat brain taken 48 hours after reproduction of ischemic stroke as described in 11. 5-6μm-thick sections cut from paraformaldehyde-fixed, paraffin-embedded tissue blocks. Use charged and precleaned slides.
Minchew, C. L., Didenko, V. V. Assessing Phagocytic Clearance of Cell Death in Experimental Stroke by Ligatable Fluorescent Probes. J. Vis. Exp. (87), e51261, doi:10.3791/51261 (2014).