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Produktion, karakterisering og potentielle anvendelse af en 3D-Tissue-manipuleret humant Esophageal Mucosal Model
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Chapters
- 00:05Title
- 02:05Human Esophageal Epithelial Cell and Fibroblast Isolation
- 04:08De-cellularized Pig Esophageal Scaffold Preparation
- 06:27Human Esophageal Mucosa Model Preparation
- 09:44Results: Representative 3D Tissue-engineered Human Esophageal Mucosa
- 11:26Conclusion
Dette manuskript beskriver produktion, karakterisering og potentielle anvendelser af et manipuleret væv 3D esophageal konstruktion fremstillet af normal primære humane esophageal fibroblast og pladeepitelceller podet i en de-cellularized porcint stillads. Resultaterne demonstrerer dannelsen af et modent stratificeret epitel ligner den normale menneskelige spiserøret.
Tags
Esophageal MucosaEsophageal AdenocarcinomaBarrett’s MetaplasiaEsophageal FibroblastsEsophageal Epithelial CellsAcellular Esophageal ScaffoldImmunohistochemical CharacterizationCK4CK14Ki67InvolucrinBiologically Relevant Experimental ModelPharmacological AgentsEnvironmental FactorsAlcoholToxinsHigh TemperatureGastro-esophageal RefluxExtended CultureEsophageal Tumor Cell LinesBarrett’s Cell LinesTumor InvasionDrug Responsiveness
