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Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity
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Bioengineering
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JoVE Journal Bioengineering
Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity

Development of a Multicellular Three-dimensional Organotypic Model of the Human Intestinal Mucosa Grown Under Microgravity

DOI:
10.3791/54148-v

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08:54 min

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July 25, 2016

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, ,
1Center for Vaccine Development, Department of Pediatrics,University of Maryland School of Medicine, 2Mucosal Immunology and Biology Research Center,Massachusetts General Hospital for Children

Chapters

  • 00:05Title
  • 00:55Preparation of Culture Vessels
  • 01:38Preparation of Cell Suspension
  • 02:49Preparation of Cell-containing Collagen Gels
  • 07:33Results: 3D Organotypic Model of Human Instestinal Mucosa
  • 07:57Conclusion

Summary

Automatic Translation

Automatic Translation

Cells growing in a three-dimensional (3-D) environment represent a marked improvement over cell cultivation in 2-D environments (e.g., flasks or dishes). Here we describe the development of a multicellular 3-D organotypic model of the human intestinal mucosa cultured under microgravity provided by rotating-wall-vessel (RWV) bioreactors.

Tags

Keywords: 3D Organotypic ModelHuman Intestinal MucosaMicrogravityRotating Wall Vessel BioreactorsEndothelial CellsFibroblastsCollagenLamininFibronectinHeparin Sulfate ProteoglycanCell Culture

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