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Behandling af menneskelige hjerte væv mod ekstracellulære Matrix selv samle Hydrogel for In Vitro og In Vivo applikationer
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1Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, 2Berlin-Brandenburg Center for Regenerative Therapies (BCRT), 3German Center for Cardiovascular Research (DZHK), 4Deutsches Herzzentrum Berlin (DHZB), 5Department of Cardiovascular Surgery, Charité – Universitätsmedizin Berlin,corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health
Chapters
- 00:05Title
- 00:43Sectioning Human Myocardium into Slices
- 02:27Decellularization of Human Myocardium Slices
- 04:20Processing the Decellularized cECM to a Powder
- 05:50Enzyme-based Homogenization of cECM Micro-particles
- 06:56Results: Effective Removal of Cellular Material Enhances Analysis
- 08:10Conclusion
Denne protokol beskriver den komplette decellularization af menneskelige myokardiet samtidig bevare sin ekstracellulære matrix komponenter. Videreforarbejdning af ekstracellulære matrix resulterer i produktionen af mikropartikler og en cytoprotective selvsamlende hydrogel.
