Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Directed Differentiation of Primitive and…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells
 

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Article DOI: 10.3791/55196-v • 14:37 min • November 1st, 2017
November 1st, 2017 •
1, 1
1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

Chapters

Summary
Automatic Translation

Here, we present human pluripotent stem cell (hPSC) culture protocols, used to differentiate hPSCs into CD34+ hematopoietic progenitors. This method uses stage-specific manipulation of canonical WNT signaling to specify cells exclusively to either the definitive or primitive hematopoietic program.

Tags

Keywords: Human Pluripotent Stem Cells Hematopoietic Progenitors Directed Differentiation Primitive Hematopoiesis Definitive Hematopoiesis Stage-specific Signal Manipulation Embryoid Bodies Trypsin EDTA Wash Buffer HPSC Media Multigas Incubator
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter