Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Efficient and Scalable Directed Differentiation…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology
Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells
 

Efficient and Scalable Directed Differentiation of Clinically Compatible Corneal Limbal Epithelial Stem Cells from Human Pluripotent Stem Cells

Article DOI: 10.3791/58279-v • 10:07 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 1
1BioMediTech Institute, Faculty of Medicine and Life Sciences, University of Tampere, 2Department of Ophthalmology, SILK, Faculty of Medicine and Life Sciences, University of Tampere
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol introduces a simple two-step method for differentiating corneal limbal epithelial stem cells from human pluripotent stem cells under xeno- and feeder cell-free culture conditions. The cell culture methods presented here enable cost-efficient, large-scale production of clinical quality cells applicable to corneal cell therapy use.

Tags

Keywords: Corneal Limbal Epithelial Stem Cells Human Pluripotent Stem Cells Feeder-free Embryoid Body Formation Basal Induction Medium SB-505124 Human Basic Fibroblast Growth Factor Limbal Stem Cell Deficiency Corneal Cell Replacement Therapy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter