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ひと心筋細胞外マトリックスの in VitroIn Vivo応用のハイドロゲルを自己組織化に向けての処理
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Processing of Human Cardiac Tissue Toward Extracellular Matrix Self-assembling Hydrogel for In Vitro and In Vivo Applications
DOI:

08:41 min

December 04, 2017

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Chapters

  • 00:05Title
  • 00:43Sectioning Human Myocardium into Slices
  • 02:27Decellularization of Human Myocardium Slices
  • 04:20Processing the Decellularized cECM to a Powder
  • 05:50Enzyme-based Homogenization of cECM Micro-particles
  • 06:56Results: Effective Removal of Cellular Material Enhances Analysis
  • 08:10Conclusion

Summary

Automatic Translation

このプロトコルでは、その細胞外マトリックス成分を維持しながらひと心筋の完全な decellularization について説明します。さらに微粒子と胃酸の自己組織化ハイドロゲルの生産における細胞外マトリックスの結果の処理。

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