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Lys arks mikroskopi for tredimensjonale visualisering av menneskelig immunceller
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Chapters
- 00:04Title
- 00:53Preparation of Neutralized Collagen
- 01:55Sample Preparation for Light-sheet Fluorescence Microscopy Using Capillaries
- 04:08Image Acquisition Using Light-sheet Microscopy
- 05:07Fixation and Immunofluorescence Staining of Cells in Collagen Matrices
- 07:17Results: Visualization of Human CTL Cells by Light-sheet Microscopy
- 08:32Conclusion
Her presenterer vi en protokoll for å visualisere immunceller i en tredimensjonal (3D) kollagen matrise med lys arks mikroskopi. Denne protokollen også utdyper hvordan spore celle migrasjon i 3D. Denne protokollen kan benyttes for andre typer suspensjon celler i 3D matrix.
