Journal
/
Immunology and Infection
/
Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Kvantificering af prolifererende humane antigen-specifikke CD4+ T-celler ved hjælp af Carboxyfluorescein Succinimidyl ester

DOI:
10.3791/59545-v

•

07:00 min

•

June 04, 2019

•

, , ,
1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Chapters

  • 00:04Title
  • 00:41Whole Blood Peripheral Blood Mononuclear Cell (PBMC) Preparation
  • 01:21CD4+ T Cell Preparation
  • 03:16CD4+ T Cell Labeling
  • 04:11Flow Cytometric Analysis
  • 05:31Results: Representative CFSE-labeled, Activated CD4+ T Cell Proliferation
  • 06:21Conclusion

Summary

Automatic Translation

Automatic Translation

Præsenteret her er en protokol til måling af prolifererende CD4+ T- celler som reaktion på antigene proteiner eller peptider ved hjælp af Dye fortynding. Denne analyse er særligt følsom over for sjældne antigen-specifikke T-celler og kan modificeres for at lette kloning af antigen-specifikke celler.

Tags

CD4+ T CellsProliferationCFSEAntigen-specificQuantificationAutoimmune ConditionsType 1 DiabetesPBMCDensity Gradient CentrifugationCell CultureFlow Cytometry

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/59545?article_type=v

Antigen Specific In Vivo Killing Assay using CFSE Labeled Target Cells
check_url/59545?article_type=v

The Use of Carboxyfluorescein Diacetate Succinimidyl Ester (CFSE) to Monitor Lymphocyte Proliferation
check_url/59545?article_type=v

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
check_url/59545?article_type=v

Adenoviral transduktion af Naive CD4 T-celler til at studere Treg Differentiering
check_url/59545?article_type=v

Isolering og Th17 Differentiering af Naïve CD4 T-lymfocytter
check_url/59545?article_type=v

Vurdering af Innate Sensing af HIV-1 inficerede CD4<sup> +</sup> T-celler ved plasmacytoide dendritiske celler ved hjælp af en<em> Ex vivo</em> Co-dyrkningssystem.
check_url/59545?article_type=v

Killer Kunstig antigen-præsenterende celler (KaAPC) til effektiv<em> In Vitro</em> Udtømning af humane antigen-specifikke T-celler
check_url/59545?article_type=v

Generering<em> De Novo</em> Antigenspecifikke humane T-celle receptorer ved retroviral transduktion af Centric Hemichain
check_url/59545?article_type=v

Udvikling af Stem Cell-afledte antigen-specifikke regulatoriske T celler mod Autoimmunitet
check_url/59545?article_type=v

In vitro differentiering af humane CD4 + Foxp3 + Induced Regulatory T-celler (iTregs) fra naive CD4 + T-celler under anvendelse et TGF-β-holdige protokol

Read Article