Materials
Name | Company | Catalog Number | Comments |
10x PBS | Bioshop | PBS405 | |
37% formaldehyde | Bioshop | FOR201 | |
Alexa Fluor 488 (goat) secondary | Invitrogen | A-11055 | use at 1:500 |
Alexa Fluor 555 (mouse) secondary | Invitrogen | A-31570 | use at 1:500 |
Alexa Fluor 647 (guinea pig) secondary | Invitrogen | A-21450 | use at 1:500 |
Alexa Fluor 647 (rat) secondary | Invitrogen | A-21247 | use at 1:500 |
Cover slips | VWR | 48366-067 | |
Dissecting forceps - #5 | Dumont | 11251-10 | |
Dissecting forceps - #55 | Dumont | 11295-51 | |
Dissection Dish | Corning | 722085 | |
Dry wipes | Kimbery Clark | 34155 | |
Goat anti-Bgal primary antibody | Biogenesis | use at 1:1000 | |
Guinea pig anti-Bsh primary antibody | Gift from Claude Desplan | use at 1:500 | |
Guinea pig anti-Vsx1 primary antibody | Erclik et al. 2008 | use at 1:1000 | |
Laboratory film | Parafilm | PM-996 | |
Microcentrifuge tubes | Sarstedt | 72.706.600 | |
Microscope slides | VWR | CA4823-180 | |
Mouse anti-dac primary antibody | Developmental Studies Hybridoma Bank (DSHB) | mabdac2-3 | use at 1:20 |
Mouse anti-eya primary antibody | DSHB | eya10H6 | use at 1:20 |
Mouse anti-nc82 primary antibody | DSHB | nc82 | use at 1:50 |
Mouse anti-svp primary antibody | DSHB | Seven-up 2D3 | use at 1:100 |
Polymer Clay | Any type of clay can be used | ||
Rabbit anti-GFP | Invitrogen | A-11122 | use at 1:1000 |
Rat anti-DE-Cadherin primary antibody | DSHB | DCAD2 | use at 1:20 |
Slowfade mounting medium | Invitrogen | S36967 | Vectashield mounting medium ( cat# H-1000) can also be used |
Triton-x-100 | Bioshop | TRX506 |
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