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Horizontal Slice Preparation of the Retina
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Horizontal Slice Preparation of the Retina

Horizontal Slice Preparation of the Retina

DOI:
10.3791/108-v

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10:45 min

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November 20, 2006

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, ,
1Dpt of Physiology and Biophysics,Dalhousie University, 2Massachusetts General Hospital,Harvard Medical School

Chapters

  • 00:05Tools
  • 00:22Preparation of agar block and agarose gel
  • 00:51Isolation of retina
  • 02:06Preparation of retina slices
  • 10:17Examples: cell layer of retina

Summary

자동 번역

자동 번역

Traditionally the vertical slice and the whole-mount preparation of the retina have been used to study the function of retinal circuits. Here, we describe the novel slicing method to preserve the dendritic morphology of retinal neurons intact.

Tags

Horizontal SliceRetinaRetinal CircuitsAmacrine CellsDendritesVertical SliceWhole-mount PreparationMorphologyPatch-clamp RecordingsGlial CellsMueller CellsSlicing MethodVibratome SlicerLow-temperature Melting Agarose GelFunction Of Retinal NeuronsCalcium Imaging TechniqueImmunocytochemistrySingle-cell RT-PCR

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