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视网膜水平切片制备

JoVE 신문
생물학

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JoVE 신문 생물학

Horizontal Slice Preparation of the Retina

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视网膜水平切片制备

DOI:

•

10:45 min

•

November 20, 2006

•

Ryosuke Enoki, Tatjana C. Jakobs, Amane Koizumi

¹Dpt of Physiology and Biophysics,Dalhousie University, ²Massachusetts General Hospital,Harvard Medical School

Chapters

00:05Tools
00:22Preparation of agar block and agarose gel
00:51Isolation of retina
02:06Preparation of retina slices
10:17Examples: cell layer of retina

Summary

자동 번역

传统的垂直切片和视网膜的整个安装准备视网膜电路的功能已被用来研究。在这里，我们描述了新的切割方法，保存完整的视网膜神经细胞的树突状形态。

Tags

Horizontal Slice Retina Retinal Circuits Amacrine Cells Dendrites Vertical Slice Whole-mount Preparation Morphology Patch-clamp Recordings Glial Cells Mueller Cells Slicing Method Vibratome Slicer Low-temperature Melting Agarose Gel Function Of Retinal Neurons Calcium Imaging Technique Immunocytochemistry Single-cell RT-PCR

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