JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:43Microarray Slide Pre-Wash
- 01:05Pre-Hybridization
- 01:31Post Pre-Hybridization
- 03:27Post-Hybridization Wash
- 05:56Imaging
- 06:39Conclusion
Het cDNA microarray PtGen2 is ontwikkeld voor genexpressie studies in loblolly pine,<em> P. taeda</em>, En andere naaldbomen. Hier tonen we pre-en post-hybridisatie hanteren en wassen technieken die gebruikt kunnen worden met deze array betere consistentie, verminderde artefacten, en een lagere opbrengst achtergronden.
Tags
Loblolly Pine PtGen2CDNA MicroarrayGene ExpressionConifer SpeciesGene DiscoveryNeedleStemCy-dye Labeled Conifer Target CDNAsAmplified And Non-amplified Indirect Labeling MethodsHybridizationWashing ConditionsHandling SlidesPre-hybridizationHybridization ProceduresTarget CDNA GenerationLabeling And Cleanup ProtocolsSoftware For Data ProcessingProprietary Print BufferSDS SolutionSalt RemovalLifterSlips™Labeled CDNA Loading
