JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:45Introduction
- 01:17Preparation of the Bait and SILAC Control Strain
- 02:44Grinding of Cells
- 04:05Pre-clearing Cell Lysate
- 05:51Eluting From IgG Beads by TEV Protease Cleavage
- 06:45Trichloroacetic Acid (TCA) Precipitation
- 08:11EtOH/ Acetate Precipitation
- 09:00Sample Analysis by Western Blotting and/ or Silver Staining
- 09:30Representative Results/ Outcome
- 10:15Conclusion
Tags
Protein ComplexesQuantitative ProteomicsS. CerevisiaeLipidsCellular MembranesCompartmentalizationIntracellular Signaling MoleculesNonvesicular RoutesMembrane Contact Sites (MCSs)Endoplasmic Reticulum (ER)Plasma Membrane (PM)Lipid-synthesizing EnzymesLipid TrafficER-PM MCSsYeast ModelEukaryotesScs2pMolecular PartnersGel ElectrophoresisIn-gel DigestionMass Spectrometry
