JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:45Types of beads: Affi-Gel and Heparin Acrylic
- 01:13Affi-Gel bead placement
- 01:18Preparation of glass capillaries
- 03:47Preparation of Affi-Gel beads
- 09:32Affi-Gel bead placement on explant
- 11:27Preparation of acrylic beads
- 13:31Acrylic bead placement on explant
- 14:32Example of obtained results
This video demonstrates two methods for preparing and placing beads, which have been coated with growth factor, on explants of the developing cerebral cortex. These beads can be used to induce spatially restricted gene expression on developing neural tissue such as forebrain explants. Methods are given for using both Affi-Gel beads and heparin acryllic beads.
