JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:55Introduction
- 01:49An Optical Projection Tomography Microscope
- 04:51Preparing Tissue for Tomographic imaging
- 09:30Absorption Reconstructions
- 11:36Fluorescent Reconstructions
- 15:44Conclusion
We suggest a Born normalized approach for Optical Projection Tomography (BnOPT) that accounts for the absorption properties of imaged samples to obtain accurate and quantitative fluorescence tomographic reconstructions. We use the proposed algorithm to reconstruct the fluorescence molecular probe distribution within small animal organs.
Tags
Born NormalizationFluorescence Optical Projection TomographyWhole Heart ImagingThree-dimensional Imaging Technique발생학Gene Expression StudiesOptical TransparencyBenzyl AlcoholBenzyl BenzoateBABBMurray’s Clear SolutionClearing ProcessScattering ContributionAbsorption ContributionFluorescence DistributionImage ArtifactsQuantification ErrorsExogenous Contrast AgentsEndogenous Contrast
