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Chapters
- 00:00Title
- 00:55Introduction
- 01:58Two-Dimensional (2-D) Gel Electrophoresis Using Cup-Loading
- 06:30Colloidal Coomassie staining with CBB G-250
- 09:21Representative Results
- 09:47Conclusion
Este vídeo se popularizar un Coomassie coloidal G-250 protocolo de tinción de acuerdo con Kang<em> Et al.</em> Para la detección de la proteína promedio de 4 ng en geles. La tinción se completa en 2 horas y sin ningún esfuerzo. Nosotros usamos el protocolo de Kang para fines de análisis en gel a base de la proteómica.
Tags
Coomassie Brilliant Blue G-250Protein StainingSDS-PAGESilver StainingFluorescence StainingMass Spectrometric Protein IdentificationAnalytical Gel-based Proteomic ApproachesSensitivity EnhancementLow-abundant ProteinsColloidal ParticlesMethanolAmmonium SulfatePhosphoric AcidBlue Silver StainingKang Et Al.Aluminum SulfateEthanol
