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病毒融合动力学测量方法在单粒子能级
JoVE 신문
생물학
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JoVE 신문 생물학
Method for Measurement of Viral Fusion Kinetics at the Single Particle Level
DOI:

14:59 min

September 07, 2009

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Chapters

  • 00:04Title
  • 00:49Introduction
  • 01:12Preparing Dextran-Functionalized Coverslips
  • 04:29Preparing the Liposomes
  • 06:41Setting Up the Microfluidic Flow Cell
  • 08:28Labeling the Virus Particles
  • 10:16Observing Viral Fusion Kinetics Under the Microscope
  • 13:10Viral Hemifusion and Pore Formation Data: Microscope Footage, Fluorescence Intensity Trajectories, and Lag Times for Viral Hemifusion and Pore Formation
  • 14:30Conclusion

Summary

자동 번역

我们提出了一个<em在体外,</em>两色荧光分析法的可视化与流体的目标双层单个病毒粒子的融合。通过标记荧光基团的差异染色病毒的细胞膜和其内部的病毒颗粒,我们是能够监测hemifusion和孔隙形成的动力学。

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