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Chapters
- 00:04Title
- 00:49Introduction
- 01:12Preparing Dextran-Functionalized Coverslips
- 04:29Preparing the Liposomes
- 06:41Setting Up the Microfluidic Flow Cell
- 08:28Labeling the Virus Particles
- 10:16Observing Viral Fusion Kinetics Under the Microscope
- 13:10Viral Hemifusion and Pore Formation Data: Microscope Footage, Fluorescence Intensity Trajectories, and Lag Times for Viral Hemifusion and Pore Formation
- 14:30Conclusion
We present an in vitro, two-color fluorescence assay to visualize the fusion of single virus particles with a fluid target bilayer. By labeling viral particles with fluorophores that differentially stain the viral membrane and its interior, we are able to monitor the kinetics of hemifusion and pore formation.
