JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:41Introduction
- 01:18Isolation of Endothelial Colony-Forming Progenitor Cells (ECFCs)
- 05:46Harvesting the ECFCs
- 08:19ECFC Expansion
- 10:57Representative ECFC Preparations
- 11:45Conclusion
Endothelial colony forming progenitor cells (ECFCs) are a promising tool to study vascular homeostasis and repair.1,2 This paper introduces a novel animal-serum free method for isolation and expansion of ECFC from heparinised adult human peripheral blood with pooled human platelet lysate (pHPL) diminishing the risk of anti-bovine immunisation.
Tags
IsolationLarge Scale ExpansionAdult HumanEndothelial Colony Forming Progenitor CellsRecovery StrategyHeparinized BloodPeripheral BloodPHPLBovine Serum AntigensFlow CytometryImmunohistochemistryIn Vitro FunctionalityVascular StructuresMatrigel AssaySubcutaneous InjectionMouse ModelPerfused VesselsCryopreservationProliferationGenomic StabilityAnimal-protein Free Isolation
