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Chapters
- 00:00Title
- 00:35Introduction
- 00:58Material Preparation and Sterilization
- 02:45Cell Preparation
- 04:56Hydrogel Formation
- 06:25Alternative Sequential Hydrogel Crosslinking
- 07:17Encapsulating Cells in 3D Gels Outcome and Applications
- 09:08Conclusion
We present protocols for the 3-dimensional (3D) encapsulation of cells within synthetic hydrogels. The encapsulation procedure is outlined for two commonly used methods of crosslinking (michael-type addition and light-initiated free radical mechanisms), as well as a number of techniques for assessing encapsulated cell behavior.
