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从小鼠胚胎干细胞的造血干细胞的推导
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JoVE 신문 생물학
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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从小鼠胚胎干细胞的造血干细胞的推导

DOI:
10.3791/162-v

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22:06 min

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February 25, 2007

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,
1Childrens Hospital, Harvard Stem Cell Institute,Harvard Medical School

Chapters

  • 00:06Assembling handing drops differentiation culture
  • 00:08Collect undifferentiated ES cells
  • 02:24Prepare materials
  • 02:57Assemble hanging drop culture
  • 04:34Collecting differentiated ES cells form hanging drops
  • 06:05Starting EB culture
  • 06:53EB dissociation and infection
  • 11:25Preparation of ES cell-derived progenitors for FACS
  • 15:18Transplantation
  • 16:19Discussion

Summary

자동 번역

자동 번역

该协议的细节从小鼠胚胎干细胞（ESC），并随后注入到致死剂量照射的的受体小鼠的造血干细胞移植的推导。简言之，电子调速器是有区别的胚体，然后用逆转录病毒HOXB4 OP9基质细胞和造血细胞因子共同培养的感染。

Tags

Stem CellsHematopoietic Stem CellsMurine Embryonic Stem CellsSelf-renewalDifferentiated ProgenyPluripotentDifferentiative AbilityClinically Useful Cell TypesVideo ProtocolHematopoietic MalignanciesHematopoietic DisordersHSC TherapyAlternative Source Of HSCBaselineHuman ESC IsolationEmbryoid Bodies (EBs)Serum Pre-screenedHematopoietic DifferentiationRetroviral HoxB4OP9 StromaBone Marrow Stromal Cell LineCytokines

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